E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir.

Abstract
Pseudomonas aeruginosa is naturally resistant to wide spectrum of antibiotics in current use and infections by this bacterium have been found very refractory to chemotherapy so in this study we attempt to study the incidence and resistance pattern of pseudomonas aeruginosa in cattle. The present study was carried on 150 samples randomly collected from different farms at El-Behera and Alexandria goveronates from mastitic cows.
The samples were directly transferred to laboratory where they were prepared for bacteriological examination for isolation of pseudomonas aeruginosa , Determination of anti-bacterial sensitivity test and isolation of plasmids. Result of this study showed that 15 out of 150 samples (10%) were positive for pseudomonas aeruginosa

Result of this study showed that 15 isolates of pseudomonas aeruginosa were tested against 9 antibacterial agents. The organism was sensitive to ciprofloxacin, vancomycin.Fifteen pseudomonas aeruginosa isolates were positive for analysis by using polyacrylamide gel electrophoresis.The carried plasmids with molecular weight of 18Kbp.Ih could be concluded that Pseudomanas aerogenosa was resistant to the most antibiotics and carrying plasmid DNA. Moreover, antibiotic is important for the persistence of plasmid DNA.
Keywords : Phenotypic-genotypic-plasmids-pseudomonas

Key words: Phenotypic-genotypic-plasmids-pseudomonas


 
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Pubmed Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. 2015; 44(1): 80-85. doi:10.5455/ajvs.1619


Web Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. https://www.alexjvs.com/?mno=1619 [Access: December 08, 2023]. doi:10.5455/ajvs.1619


AMA (American Medical Association) Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. 2015; 44(1): 80-85. doi:10.5455/ajvs.1619



Vancouver/ICMJE Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. (2015), [cited December 08, 2023]; 44(1): 80-85. doi:10.5455/ajvs.1619



Harvard Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir (2015) Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS, 44 (1), 80-85. doi:10.5455/ajvs.1619



Turabian Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. 2015. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. Alexandria Journal of Veterinary Sciences, 44 (1), 80-85. doi:10.5455/ajvs.1619



Chicago Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. "Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis." Alexandria Journal of Veterinary Sciences 44 (2015), 80-85. doi:10.5455/ajvs.1619



MLA (The Modern Language Association) Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir. "Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis." Alexandria Journal of Veterinary Sciences 44.1 (2015), 80-85. Print. doi:10.5455/ajvs.1619



APA (American Psychological Association) Style

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir (2015) Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. Alexandria Journal of Veterinary Sciences, 44 (1), 80-85. doi:10.5455/ajvs.1619





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