E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
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Original Article 


Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah.

Abstract
In this study, a total number of animal samples (583) were collected from cattle (170) , sheep (221) and goats (192) suffering from respiratory manifestations (freshly dead and slaughtered). Nasal swabs (169) were collected from cattle (43), sheep (67) and goats (59) . Blood samples (312) were collected from cattle (95) , sheep (112) and goats (105) and lung and spleen tissues (102) were collected from cattle (32) ,sheep (42) and goats (28) . Samples collected for bacteriological isolation and identification of P. multocida. After that confirmation of identification by using PM-PCR, and multiplex-PCR for detection of the virulence factors genes, DNA nucleotides sequencing of these genes were done for detection of the genetic diversity.
The prevalence of P.multocida isolated from all samples from Cattle, Sheep and Goats is 1.76%, 1.8%, 1.56% respectively. While, P. multocida not found in all blood samples collected from cattle, sheep and goat.
PM-PCR and multiplex-PCR confirmed presence of genes under examination.
DNA nucleotide sequencing and analysis of sequences obtained for the 5 genes under investigation Concluded that: P. multocida isolates had no genetic diversity of oma 87 gene, ptf A gene, phf A, tox A and nan B among cattle, sheep and goats.

Key words: Genetic diversity, Pasteurella multocida, virulence factors genes.


 
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How to Cite this Article
Pubmed Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961

Web Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. https://www.alexjvs.com/?mno=263961 [Access: December 08, 2023]. doi:10.5455/ajvs.263961

AMA (American Medical Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961


Vancouver/ICMJE Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. (2017), [cited December 08, 2023]; 54(1): 173-178. doi:10.5455/ajvs.263961


Harvard Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS, 54 (1), 173-178. doi:10.5455/ajvs.263961


Turabian Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. 2017. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961


Chicago Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. "Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species." Alexandria Journal of Veterinary Sciences 54 (2017), 173-178. doi:10.5455/ajvs.263961


MLA (The Modern Language Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah. "Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species." Alexandria Journal of Veterinary Sciences 54.1 (2017), 173-178. Print. doi:10.5455/ajvs.263961


APA (American Psychological Association) Style

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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