E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability

Amira H. El-Baz.

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  • Abstract
    Staphylococcus aureus has the ability to yield heat stable enterotoxins resulting in food intoxication in human with variable intensity. So, the objectives of this research are to investigate the incidence of Staphylococcus aureus in Egyptian Soft Cheese (Talaga cheese), molecular identification of nuc and mecA genes in isolated strains by Multiplex PCR, assess the antimicrobial resistance profile using 16 different antibiotics as well as evaluate the Antimicrobial activity of rosemary oil on the viability of S. aureus. Sixty random samples of Talaga soft cheese were collected from different market places and dairy shops in various districts of Mansoura town, Dakahlia governorate. Egypt. S. aureus was discovered in 30% (18/60) from examined samples where 4 isolates (22.2%) were proved to be enterotoxigenic strains. All S. aureus isolates contained on nuc gene, however the mecA gene was found only in 5 S. aureus isolates (27.7%). S. aureus isolates showed a higher multiple antibiotic resistance (MAR) where the average MAR index was 0.479 and this leads to an increase in the public health hazard. The antimicrobial properties of rosemary essential oil as a natural antimicrobial agent were evaluated against S. aureus strain (ATCC 25923). It could be concluded that using rosemary essential oil with 2% concentration was highly effective in reducing and inhibiting S. aureus.

    Key words: Egypt, Talaga cheese, nuc, mecA, Rosemary oil, Staphylococcus aureus


     
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    Pubmed Style

    Amira H. El-Baz. Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. AJVS. 2022; 73(2): 35-46. doi:10.5455/ajvs.104387


    Web Style

    Amira H. El-Baz. Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. https://www.alexjvs.com/?mno=104387 [Access: July 31, 2022]. doi:10.5455/ajvs.104387


    AMA (American Medical Association) Style

    Amira H. El-Baz. Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. AJVS. 2022; 73(2): 35-46. doi:10.5455/ajvs.104387



    Vancouver/ICMJE Style

    Amira H. El-Baz. Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. AJVS. (2022), [cited July 31, 2022]; 73(2): 35-46. doi:10.5455/ajvs.104387



    Harvard Style

    Amira H. El-Baz (2022) Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. AJVS, 73 (2), 35-46. doi:10.5455/ajvs.104387



    Turabian Style

    Amira H. El-Baz. 2022. Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. Alexandria Journal of Veterinary Sciences, 73 (2), 35-46. doi:10.5455/ajvs.104387



    Chicago Style

    Amira H. El-Baz. "Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability." Alexandria Journal of Veterinary Sciences 73 (2022), 35-46. doi:10.5455/ajvs.104387



    MLA (The Modern Language Association) Style

    Amira H. El-Baz. "Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability." Alexandria Journal of Veterinary Sciences 73.2 (2022), 35-46. Print. doi:10.5455/ajvs.104387



    APA (American Psychological Association) Style

    Amira H. El-Baz (2022) Prevalence, Molecular Characterization, Antibiotics Resistance of Staphylococcus aureus Isolated from Egyptian Soft Cheese, and Effect of Rosemary Essential Oil on its Viability. Alexandria Journal of Veterinary Sciences, 73 (2), 35-46. doi:10.5455/ajvs.104387





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