E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky.

Abstract
The objective of this study was to investigate the molecular characterization and antibiogram of Photobacteria damselae isolated from marine cultured fish. A total number of 578 cultured marine fish (321 Seabass Dicentrarchus labrax and 257 Seabream Sparus aurata) were obtained from Alexandria governorate farms and subjected to microbiological investigation for differentiation of Photobacteria damselae subspecies. The occurrence of Photobacteria damselae subspecies piscicida and Photobacteria damselae subspecies damselae were higher in Seabream S. aurata 44.47% and 26.46% than Seabass D. labrax 38.32% and 16.20% respectively. The seasonal prevalence was the highest during summer 23.36% and 54.2% and the lowest in winter 4.49% and 6.67% respectively. The multiplex PCR assay was used to differentiate between P. d. subsp. pisci¬cida and subsp. damselae. It was based on a pair of 16S rRNA gene- PCR primers, namely CAR-1 and CAR-2 in addition to ureC gene binding primers. The P. d. subsp. damselae strains have yielded two amplification products 267 and 448 bp. The P. d. subsp. piscicida strains showed 267 bp only. Antibiogram sensitivity showed by P. damselae subsp. piscicida and P. damselae subsp. damselae strains were sen¬sitive to Sulphamethoxazole andTrimethoprim and Florofenicol.

Key words: Photobacteriosis–Seabass-Seabream- Occurrence- Molecular-Antibiogram.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Habiba M. Essam
Articles by Safaa I. Tayel
Articles by Helmy A. Torky
on Google
on Google Scholar
Article Statistics
 Viewed: 4834
Downloaded: 727


REFERENCES
Abdel-Aziz, M., Eissa, A. El., Hanna, M., Abou Okada, M. 2013. Identifying some pathogenic Vibrio/Photobacterium species during mass mortalities of cultured Gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) from some Egyptian coastal provinces. Int. J. Vet. Sci. Med. 1: 87–95. [DOI via Crossref]   
Acosta, F., Ellis, A. E., Vivas, J., Padilla, D., Acosta, B., De’niz, S., Bravo, J. and Real, F., 2006. Complement consumption by Photobacterium damselae subsp. piscicida in seabream, red porgy and seabass normal and immune serum. Fish.Shellfish Immu. 20: 709-717. [DOI via Crossref]    [Pubmed]   
Austin, B. and Austin, D.A., 1999. Bacterial Fish Pathogens of Farmed and Wild Fish, 3rd ed. (revised). Praxis Publ., Chichester, UK.457 pp.
Bakopoulos, V., Adams, A. and Richards, R.H., 1995. Some biochemical proporties and antibiotic sensitivities of Pasteurella piscicida isolated in Greece and comparison with strains from Japan, France and Italy. J. Fish.
Bakopoulos, V., Peric, Z., Rodger, H., Adams, A. and Richards, R.H., 1997. First report of fish pasteurellosis from Malta. J. Aqua. Anim. Health 9: 26–33. [DOI via Crossref]   
Baptista, T., Romalde, J.L. and Toranzo, A.E., 1996. First occurrence of pasteurellosis in Portugal affecting cultured gilthead Seabream Sparus aurata. Bull. Eur. Ass. Fish Pathol. 16:92–95.
Buller, N.B., 2004. Bacteria from Fish and other aquatic animal/a practical identification manual/ Nicky B. Buller.P:137-177 [DOI via Crossref]   
Candan, A., Kucuker, M. A. and Karatas, 5., 1996. Pasteurellosis in cultured seabass (Dicentrarchus labrax) in Turkey. Bull Eur Assoc Fish Path .6: 150-153.
Fouz, B., Larsen, J. L., Nilsen, B., Barja, J. L. and Toranzo, A. E., 1992. Characterization of Vibrio damsela strains isolated from turbot Scophthalmus maximus in Spain. Dis. Aqua. Organis., 12: 155-166. [DOI via Crossref]   
Gauthier, G., Lafay, B., Ruimy, R., Breittmayer, V., Nicolas, J. L. and Gauthier, M., 1995. Small-subunit rRNA sequences and whole DNA relatedness concur for the reassignment of Pasteurella piscicida (Sniezko et al.)Janssen and Surgalla to the genus Photobacterium as Photobacterium damsela subsp. piscicida comb. nov. Internat.J. Syst. Bact., 45:139-144. [DOI via Crossref]   
Ghinsberg, R. C., Drasinover, V., Sheinberg, Y. and Nitzan, Y., 1995. Seasonal distribution of Aeromonas hydrophila and Vibrio species in Mediterranean coastal water and beaches: a possible health hazard. Biomedical Letters, 51: 151-159.
Korun, J., Timur, G., 2005. The first Pasteurellosis case in cultured Seabass (DicentrarchusLabrax L.) at low marine water tempretures in Turkey. J. Aquaculture – Bamidgeh 57(3):197-206.
Labella, A., Vida, M., Alonso, M. C., Infante, C., Cardenas, S., Lopez-Romalde, S., Manchado, M. and Borrego, J. J., 2006. First isolation of Photobacterium damselae ssp. damselae from cultured redbanded seabream, Pagrus auriga Valenciennes, in Spain. J. Fish Dis. 29:175–179. [DOI via Crossref]    [Pubmed]   
Labella, A., 2010. Aislamiento y Caracterizaciَn de Bacterias Potencialmente PatَgenasAsociadas a NuevasEspecies de EspلridosCultivados, PhD Thesis, University of Malaga, Spain.
Liu, P., Hung, S.W, Ming, H.,Cheng, C.F., Cheng, C.L., Chang C.H., Lin, S.L., Tu, C.Y., Lin, Y.H. and Wang, W.S., 2011. Highly virulent Photobacterium damselae subsp piscicida isolated from Taiwan paradise fish, Macropodus opercularis (L.), in Taiwan. African J. Microb. Res. Vol. 5(15) : 2107-2113.
Magarin˜os, B., Toranzo, A.E. and Romalde, J.L., 1996. Phenotypic and pathobiological characteristics of Pasteurella piscicida. Annual Rev. Fish Dis. 6, 41–64.
Marzouk, M.S., Hanna, M.I. and Kenawy, A. M., 2009. Monitoring the Cause of Mortality in Some Marine Fishes in Matrouh Governorate, Egypt during the summer 2008. American-Eurasian J. Agric. Environ. Sci., 5 (2): 148-158.
NCCLS, 2001. Performance Standards for Antimicrobial Susceptibility Testing, 11th Supplement. National Committee for Clinical Laboratory Standards of Antimicrobial Susceptibility Testing, Wayne.
Osorio, C.R., Collins, M.D., Toranzo, A.E., Barja, J.L. and Romalde, J.L., 1999.16S rRNA gene sequence analysis of Photobacterium damselae and nested PCR method for rapid detection of the causative agent of fish pasteurellosis. Appl. Environ. Microbiol.65:2942– 2946. [Pubmed]    [PMC Free Fulltext]   
Osorio, C.R., Toranzo, A.E., Romalde, J.L. and Barja, J.L., 2000. Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae. Dis. Aqua.Organis. 40:177–183. [DOI via Crossref]    [Pubmed]   
Romalde, J.L. 2002. Photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen. Intern. Microb. 5: 3–9. [DOI via Crossref]    [Pubmed]   
Shin J.D., Shin M.G., Suh S.P., Ryang D.W., Rew J.S. and Nolte F.S., 1996. Primary Vibrio damsela septicemia. Clini. Infect. Dis. (22): 856–857
Thyssen, A. and Ollevier, F., 2001. In vitro antimicrobial susceptibility of Photobacterium damselae subsp. piscicida to 15 different antimicrobial agents. Aquacul .200:259–269. [DOI via Crossref]   
Thyssen, A., Grisez, L., VanHoudt, R. and Ollevier, F., 1998. Phenotypic characterization of the marine pathogen Photobacterium damselae subsp. piscicida. Intern.J. Syst. Bact .48: 1 145-1 1 51.
Toranzo, A.E., Barreiro, S., Casal, J.F., Figueras, A., MagarinËœos, B. and Barja, J.L., 1991. Pasteurellosis in cultured gilthead seabream (Sparus aurata): first report in Spain. Aquacul. 99:1–15. [DOI via Crossref]   
Zorrilla, I., Balebona, M.C., Mori-igo, M.A., Saradquete, C. and Borrego, J.J., 1999. Isolation and characterization of the causative agent of pasteurellosis, Photobacterium damselae subsp. piscicida, from sole, Solea senegalensis (Kaup), J. Fish Dis., 22: 167-172. [DOI via Crossref]   

How to Cite this Article
Pubmed Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. AJVS. 2015; 44(1): 103-110. doi:10.5455/ajvs.129


Web Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. http://www.alexjvs.com/?mno=129 [Access: February 08, 2020]. doi:10.5455/ajvs.129


AMA (American Medical Association) Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. AJVS. 2015; 44(1): 103-110. doi:10.5455/ajvs.129



Vancouver/ICMJE Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. AJVS. (2015), [cited February 08, 2020]; 44(1): 103-110. doi:10.5455/ajvs.129



Harvard Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky (2015) Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. AJVS, 44 (1), 103-110. doi:10.5455/ajvs.129



Turabian Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. 2015. Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. Alexandria Journal of Veterinary Sciences, 44 (1), 103-110. doi:10.5455/ajvs.129



Chicago Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. "Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish." Alexandria Journal of Veterinary Sciences 44 (2015), 103-110. doi:10.5455/ajvs.129



MLA (The Modern Language Association) Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky. "Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish." Alexandria Journal of Veterinary Sciences 44.1 (2015), 103-110. Print. doi:10.5455/ajvs.129



APA (American Psychological Association) Style

Habiba M. Essam, Safaa I. Tayel, Helmy A. Torky (2015) Occurrence and Characterization of Photobacteria Damselae in Cultured Marine Fish. Alexandria Journal of Veterinary Sciences, 44 (1), 103-110. doi:10.5455/ajvs.129





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 8 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 7 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]