E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis

Sherif M. Shawky, Sahar H. Orabi, Gopal Reddy, Gemechu Wirtu.

Abstract
The objective of the current study was to assessment the effect of L-Carnitine as a stimulant of lipid catabolism in vitro maturation (IVM) and in vitro culture (IVC) media on IVM rate (nuclear maturation) and embryonic development. A total of 495 oocytes were assigned into two groups; the control group, oocytes matured in TCM-199 medium; L-Carnitine group oocytes matured in TCM-199 medium supplemented with L-Carnitine (0.5mg/ml). After that 250 oocytes were stained with Hoechst 33342 then with Nile Red dye. The nuclear maturation and oocyte lipid content were determined under a fluorescence microscope. The other matured oocytes were incubated for cell activation by Thimerosal and DL- Dithiothreitol (DDT). Potential zygotes were incubated in vitro culture 1 and 2, the stained embryos were counted and evaluated for morula and blastocyst stages. The Metaphase II rates were 16.2% and 42.1% in the control group and L-carnitine group, respectively. Overall, 9.3% and 14.9% of the oocytes reached at least the MI stage in the control group and L-carnitine group respectively. The addition of L-carnitine in IVM media resulted in a significant decrease in the amount of lipid in cat oocyte. Moreover, the incubation of matured oocytes of the L-carnitine group in IVC1 lead to significant increase in cleaved cells 29.4% in comparison with the control group 15.4%. The incubation of cleaved cell of the L-carnitine group in IVC2 lead to decrease significantly in degenerated cells 12.5 % compared to the control group 42.9% while caused an increase significantly in morula 50% compared to the control group 33.3% and blastocyst 37.5% comparison with the control group 23.8%. In conclusion, the use of L-Carnitine as a supplement in IVM and IVC media decrease lipid content of cat oocyte and subsequent enhance the nuclear maturation, cell activation of cat's embryo, and embryonic development.

Key words: L-carnitine; Domestic cat; Oocyte; In vitro maturation; Embryo


 
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How to Cite this Article
Pubmed Style

Shawky SM, Orabi SH, Reddy G, Wirtu G. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. AJVS. 2020; 67(1): 1-8. doi:10.5455/ajvs.138289


Web Style

Shawky SM, Orabi SH, Reddy G, Wirtu G. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. http://www.alexjvs.com/?mno=138289 [Access: November 27, 2020]. doi:10.5455/ajvs.138289


AMA (American Medical Association) Style

Shawky SM, Orabi SH, Reddy G, Wirtu G. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. AJVS. 2020; 67(1): 1-8. doi:10.5455/ajvs.138289



Vancouver/ICMJE Style

Shawky SM, Orabi SH, Reddy G, Wirtu G. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. AJVS. (2020), [cited November 27, 2020]; 67(1): 1-8. doi:10.5455/ajvs.138289



Harvard Style

Shawky, S. M., Orabi, . S. H., Reddy, . G. & Wirtu, . G. (2020) L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. AJVS, 67 (1), 1-8. doi:10.5455/ajvs.138289



Turabian Style

Shawky, Sherif M., Sahar H. Orabi, Gopal Reddy, and Gemechu Wirtu. 2020. L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. Alexandria Journal of Veterinary Sciences, 67 (1), 1-8. doi:10.5455/ajvs.138289



Chicago Style

Shawky, Sherif M., Sahar H. Orabi, Gopal Reddy, and Gemechu Wirtu. "L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis." Alexandria Journal of Veterinary Sciences 67 (2020), 1-8. doi:10.5455/ajvs.138289



MLA (The Modern Language Association) Style

Shawky, Sherif M., Sahar H. Orabi, Gopal Reddy, and Gemechu Wirtu. "L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis." Alexandria Journal of Veterinary Sciences 67.1 (2020), 1-8. Print. doi:10.5455/ajvs.138289



APA (American Psychological Association) Style

Shawky, S. M., Orabi, . S. H., Reddy, . G. & Wirtu, . G. (2020) L-Carnitine improves in vitro maturation of feline oocytes and subsequent development of embryos generated by parthenogenesis. Alexandria Journal of Veterinary Sciences, 67 (1), 1-8. doi:10.5455/ajvs.138289





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