Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat

Helmy A Torky; Hanaa A Ahmed; Emad S Omran

doi:10.5455/ajvs.185240

2025, Vol: 86

86Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 04 Aug 2015

AJVS. 2015; 46(1): 100-109

doi: 10.5455/ajvs.185240

Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat

Helmy A Torky, Hanaa A Ahmed, Emad S Omran.

Abstract
In this study, duplex real-time PCR approach with melting curve analysis is presented for the detection of Listeria monocytogenes, which are important food-borne bacterial pathogens usually present in imported frozen meat. Melting curve analysis using a SYBR Green I real-time PCR approach showed characteristic Tm values demonstrating the specific and efficient amplification of the two pathogens;73.2°C and 81.54°C for L. monocytogenes and Salmonella spp., respectively, the duplex, real-time PCR was equally sensitive to uniplex real-time PCR, using same amounts of purified DNA. When our established duplex real-time PCR assay was applied to artificially inoculated imported frozen meat the detection limit was 103 CFU/g for each of these pathogens without enrichment. The overall process included the culture enrichment based on the ISO standard, consisting of 24 h incubation in appropriate media (Half Fraser Broth for Listeria and buffered peptone water (BPW) for Salmonella), followed by a single DNA extraction of mixed enrichment aliquot. The results from this study showed that the developed duplex real-time PCR with melting curve analysis is promising as a rapid and cost-effective test method for improving food safety.

Key words: Salmonella, Listeria monocytogenes, Multiplex, Real-time PCR

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Helmy A Torky Articles by Hanaa A Ahmed Articles by Emad S Omran
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. 2015; 46(1): 100-109. doi:10.5455/ajvs.185240

Web Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. https://www.alexjvs.com/?mno=185240 [Access: May 04, 2025]. doi:10.5455/ajvs.185240

AMA (American Medical Association) Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. 2015; 46(1): 100-109. doi:10.5455/ajvs.185240

Vancouver/ICMJE Style

Torky HA, Ahmed HA, Omran ES. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS. (2015), [cited May 04, 2025]; 46(1): 100-109. doi:10.5455/ajvs.185240

Harvard Style

Torky, H. A., Ahmed, . H. A. & Omran, . E. S. (2015) Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. AJVS, 46 (1), 100-109. doi:10.5455/ajvs.185240

Turabian Style

Torky, Helmy A, Hanaa A Ahmed, and Emad S Omran. 2015. Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. Alexandria Journal of Veterinary Sciences, 46 (1), 100-109. doi:10.5455/ajvs.185240

Chicago Style

Torky, Helmy A, Hanaa A Ahmed, and Emad S Omran. "Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat." Alexandria Journal of Veterinary Sciences 46 (2015), 100-109. doi:10.5455/ajvs.185240

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Torky, H. A., Ahmed, . H. A. & Omran, . E. S. (2015) Duplex Real Time PCR for Simultaneous Detection of Salmonella Species and Listeria Monocytogenes in Frozen Meat. Alexandria Journal of Veterinary Sciences, 46 (1), 100-109. doi:10.5455/ajvs.185240

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article