E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy.

Abstract
A total number of 170 fishes (100 fresh water, 40 brackish water, and 30 marine water fishes) from different farms in Alexandria, Kafr Elsheikh, and Behira governorates were collected from different water salinities. The prevalence of A.hydrophila was 47% (38% in fresh water fish, 65% brackish water fish, 53.3% in marine water fish). A.hydrophila isolates were tested for pathogenicity by inoculation into blood agar and skimmed milk agar 1% for hemolytic and proteolytic activity.
All isolates showed β hemolysis and proteolytic activity. A.hydrophila isolates were inoculated into trypticase soya broth with different NaCl concentration (0.5, 1, 2, 3, 4, 5, 6%) and turbidity was measured by photometer at wave length 610 nm. The higher NaCl concentration, the lower turbidity was found. Moreover, reduction % of total bacterial count of A.hydrophila in relation to different concentration of NaCl in TSB after 10 fold serial dilutions by surface plating technique was calculated.
Hemolysin and aerolysin genes were detected by PCR from the isolates from different sources. A.hydrophila isolates from fresh, brackish and marine fish showed high resistance to Ampicillin ﴾M10﴿, Erythromycin ﴾E15﴿ Nalidixic acid (NA30), and Spectinomycin (SPT10). On the other hand, A.hydrophila isolates from fresh, brackish and marine fish showed high sensitivity to Enrofloxacin (EF10), Ofloxacin ﴾OFX5 ﴿and Gentamicin (CN10﴿. Polymixin ﴾PB300 u ﴿showed activity against A.hydrophila from brackish and marine fishes only. Doxycycline ﴾DO30﴿ and Nitrofurantoin ﴾F300u) were highly effective against brackish water isolates than others for the field application. Isolation of A.hydrophila from different water salinities raises the public health concern and the importance to find suitable methods to control the infection.

Key words: A.hydrophila, Fresh, Brackish, Marine fishes, PCR


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Basma Dahdouh
Articles by Ola Basha
Articles by Samy Khalil
Articles by Mahmoud Tanekhy
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. 2016; 48(2): 46-53. doi:10.5455/ajvs.208107


Web Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. https://www.alexjvs.com/?mno=208107 [Access: December 03, 2023]. doi:10.5455/ajvs.208107


AMA (American Medical Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. 2016; 48(2): 46-53. doi:10.5455/ajvs.208107



Vancouver/ICMJE Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS. (2016), [cited December 03, 2023]; 48(2): 46-53. doi:10.5455/ajvs.208107



Harvard Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy (2016) Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. AJVS, 48 (2), 46-53. doi:10.5455/ajvs.208107



Turabian Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. 2016. Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. Alexandria Journal of Veterinary Sciences, 48 (2), 46-53. doi:10.5455/ajvs.208107



Chicago Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. "Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes." Alexandria Journal of Veterinary Sciences 48 (2016), 46-53. doi:10.5455/ajvs.208107



MLA (The Modern Language Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy. "Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes." Alexandria Journal of Veterinary Sciences 48.2 (2016), 46-53. Print. doi:10.5455/ajvs.208107



APA (American Psychological Association) Style

Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy (2016) Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes. Alexandria Journal of Veterinary Sciences, 48 (2), 46-53. doi:10.5455/ajvs.208107





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles