E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology

Mohamed, A. Nossair and Yasser N. Haggag.

Abstract
A total of 300 human serum samples were examined by RBPT and c. ELISA to estimate the prevalence of brucellosis in humans in Alexandria Province and to investigate the epidemiological pattern of such disease among humans. It was found that the overall seroprevalence of brucellosis in humans through RBPT and c. ELISA was 14.67, and 11.67%, respectively. Statistical analysis showed that there was a significant association at (P<0.0001) between the seroprevalence of brucellosis among human beings in relation to place of residence where the highest prevalence was noticed in El- Aameria (18.40%) followed by Gharb (17.86%), Borg El Arab (16.66%), Sharq (13.33%), El Montazah (13.24%) and Wasat (12.5%). On contrary, all the examined samples that were collected from El- Gomrok were found to be negative. In addition, the results clarified that 25% of the examined samples collected from patients complaining of fever were found to be positive for brucellosis. On the other side, only 7.78% of the examined samples of apparently healthy individuals were found to be positive for brucellosis with significant association. Sex based seroprevalence showed that males' prevalence (16.67%) was higher than that in female (12.12%) with significant association at (P < 0.05). Also, age based seroprevalence showed that the highest prevalence was observed in the age group (20 - ˂ 40 years) (19.75%) followed by the age group (˂ 20 years) (13.48%) then the age group (40 - ˂ 60 years) (12.79%) and lastly the age group (˃ 60 years) (11.36%) with non-significant association between different age groups and prevalence of human brucellosis with significant association at (P < 0.01). Moreover, the highest seroprevalence was observed in farmers (16.30%) followed by housewives (14.25%), animals' attendants (14.10%), veterinarians (11.11%), slaughterhouse workers (8.33%) and finally veterinary students (0.59%) with significant association at (P < 0.01) between different occupations and prevalence of human brucellosis. Under the conditions in the current study and according to the data obtained, it is concluded that brucellosis is still remaining a problem in Alexandria province and it was threatening human population through direct and indirect transmission. Also, the prevalence of the disease is significantly associated with health status, sex, age and occupation while it is non-significantly associated with manner of milk consumption, locality and the type of animal contact.

Key words: Brucellosis, Human, seroprevalence, RBPT, c. ELISA


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed
Articles by A. Nossair and Yasser N. Haggag
on Google
on Google Scholar
Article Statistics
 Viewed: 1048
Downloaded: 158
Cited: 0

How to Cite this Article
Pubmed Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. 2016; 51(2): 296-302. doi:10.5455/ajvs.239565


Web Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. http://www.alexjvs.com/?mno=239565 [Access: November 15, 2018]. doi:10.5455/ajvs.239565


AMA (American Medical Association) Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. 2016; 51(2): 296-302. doi:10.5455/ajvs.239565



Vancouver/ICMJE Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. (2016), [cited November 15, 2018]; 51(2): 296-302. doi:10.5455/ajvs.239565



Harvard Style

Mohamed, A. Nossair and Yasser N. Haggag (2016) Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS, 51 (2), 296-302. doi:10.5455/ajvs.239565



Turabian Style

Mohamed, A. Nossair and Yasser N. Haggag. 2016. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. Alexandria Journal of Veterinary Sciences, 51 (2), 296-302. doi:10.5455/ajvs.239565



Chicago Style

Mohamed, A. Nossair and Yasser N. Haggag. "Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology." Alexandria Journal of Veterinary Sciences 51 (2016), 296-302. doi:10.5455/ajvs.239565



MLA (The Modern Language Association) Style

Mohamed, A. Nossair and Yasser N. Haggag. "Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology." Alexandria Journal of Veterinary Sciences 51.2 (2016), 296-302. Print. doi:10.5455/ajvs.239565



APA (American Psychological Association) Style

Mohamed, A. Nossair and Yasser N. Haggag (2016) Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. Alexandria Journal of Veterinary Sciences, 51 (2), 296-302. doi:10.5455/ajvs.239565





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Surgical and Histological Evaluation of the Effectiveness of Propolis on Wound Healing
    Ramadan El Sayed Abd Elwahed, Mahmoud Hussein El Kammar, Islam Reda Edrees
    AJVS. 2013; 39(1): 52-63
    » Abstract & References

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Improving Production Traits for El-salam and Mandarah Chicken Strains by Crossing I- Estimation of Crossbreeding Effects for Growth Production Traits
    Ayman El-Sayed Taha, Fawzy Ali Abd El-Ghany
    AJVS. 2013; 39(1): 18-30
    » Abstract & References

  • Most Downloaded
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Aerobic Bacteria Associated with Omphalitis of Chicks [English]
    Khalil, S. A. and Einas, El-Shamy
    AJVS. 2012; 37(1): 69-77
    » Abstract

  • Improving Production Traits for El-salam and Mandarah Chicken Strains by Crossing I- Estimation of Crossbreeding Effects for Growth Production Traits
    Ayman El-Sayed Taha, Fawzy Ali Abd El-Ghany
    AJVS. 2013; 39(1): 18-30
    » Abstract & References

  • Most Cited Articles
  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 3 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 3 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 3 times [Click to see citing articles]

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307
    Cited : 2 times [Click to see citing articles]

  • Enumeration and Characterization of Aeromonas spp. Isolated from Milk and Some Dairy Products in Sharkia Governorate, Egypt
    Nagwa I. ElBalat, Salah F. Abd-ElAal, Madeha A. Ayoub, Magdy S. Elsayed
    AJVS. 2014; 40(1): 52-64
    » Abstract & References » doi: 10.5455/ajvs.49073
    Cited : 2 times [Click to see citing articles]

  • Comparison of Lidocaine and LidocaineNeostigmine for Epidural Analgesia in Water Buffalo Calves (Bubalus Bubalis)
    Alaa Ghazy, Ayman Atiba, Mustafa Shukry, Tarek Kamal
    AJVS. 2015; 46(1): 177-181
    » Abstract & References » doi: 10.5455/ajvs.198171
    Cited : 2 times [Click to see citing articles]

  • Effect of Vitrification by Straw and Cryotop on DNA Integrity Using Comet Assay with Reference To Brilliant Cresyl Blue Exposure in Buffalo Oocytes
    Scholkamy T.H., Samah F. Darwish, karima M. Mahmoud
    AJVS. 2015; 46(1): 117-123
    » Abstract & References » doi: 10.5455/ajvs.187719
    Cited : 2 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 2 times [Click to see citing articles]

  • Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons
    Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.
    AJVS. 2015; 46(1): 1-14
    » Abstract & References » doi: 10.5455/ajvs.186038
    Cited : 2 times [Click to see citing articles]