E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid.

Abstract
Our a complete of 60 samples of fresh marine fishes represented by Sardine, Saurus, Barboni and Chrysophyres were collected at different times from various fish markets in Alexandria governorate, Egypt and examined for determination of their levels of histamine by HPLC from one side and ELISA from the other side to study the accuracy of the two techniques. Results showed that all of specimens contained detectable value of histamine but the highest unacceptable sample in sardine fish and lowest unacceptable sample in Chrysophyres fish and there is no significant differences (P<0.01) as indicated by HPLC and ELISA test for detection of histamine. Further, some trials to control the formation of such serious histamine level (mg %) were treated by using 1% essential oils (Rosemary oil, Garlic oil and Lemon oil) by the use of HPLC. The outcomes indicated that the development of histamine formation become simply decreased by the percentage of 15.2% ,47.8 and 58.2% with addition of Rosemary oil (1%) for 2 ,4 days and 6 days ,respectively; 9.9 % , 35.3% and 45.7% after addition of garlic oil(1%) for two ,four days and six days , respectively and the best reduction degree in histamine development changed into recorded through the addition of Lemon oil (1%) 2.1% , 21.5% and 29.3% for two ,four days and six days ,respectively. In conclusion, such results can propose that the essential oil treatment to the fish fillets might be helpful in reduction of histamine formation so that it will produce fish meat of excessive best and protection.

Key words: Histamine, HPLC, ELISA, Marine fish, Essential oil.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Reyad R. Shawish
Articles by Zakaria Hassan
Articles by Walaa Abd-Elmegid
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. AJVS. 2017; 53(2): 91-98. doi:10.5455/ajvs.265833


Web Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. https://www.alexjvs.com/?mno=265833 [Access: December 06, 2023]. doi:10.5455/ajvs.265833


AMA (American Medical Association) Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. AJVS. 2017; 53(2): 91-98. doi:10.5455/ajvs.265833



Vancouver/ICMJE Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. AJVS. (2017), [cited December 06, 2023]; 53(2): 91-98. doi:10.5455/ajvs.265833



Harvard Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid (2017) Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. AJVS, 53 (2), 91-98. doi:10.5455/ajvs.265833



Turabian Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. 2017. Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. Alexandria Journal of Veterinary Sciences, 53 (2), 91-98. doi:10.5455/ajvs.265833



Chicago Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. "Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils." Alexandria Journal of Veterinary Sciences 53 (2017), 91-98. doi:10.5455/ajvs.265833



MLA (The Modern Language Association) Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid. "Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils." Alexandria Journal of Veterinary Sciences 53.2 (2017), 91-98. Print. doi:10.5455/ajvs.265833



APA (American Psychological Association) Style

Reyad R. Shawish, Zakaria Hassan, Walaa Abd-Elmegid (2017) Comparative Study on Determination of Histamine Using HPLC and ELISA in Marine Fish and Control of Excessive Histamine in Fish Fillet by Natural Oils. Alexandria Journal of Veterinary Sciences, 53 (2), 91-98. doi:10.5455/ajvs.265833





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles