E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk

El Sayed F.H. Ibrahim, Ashraf M. Abd El wahab, Samy A. Khalil, Helmy A. Torky.

Abstract
Bacterial resistance to antibiotics of Beta lactam group is increasing worldwide threatening the public healthcare due to the limited treatment substitutions. Beta lactamase especially Extended Spectrum Beta Lactamase (ESBL) enzymes are responsible for the most important resistance mechanisms of the Enterobacteriacae to antibiotics of Beta lactam group. In this study, in order to investigate the prevalence of extended-spectrum ╬▓-lactamase among Enterobacteriacae isolated from clinically mastitis and apparently normal cattle. A total number of 500 milk samples were collected from cow milk. A total number of 100 ESBL Enterobacteriaceae isolates were obtained. Bacterial isolates were identified by VITEK 2 compact and then ESBL enzymes were identified phenotypically. Estimation of sensitivity of these isolates against variety of antibiotics by Disk Diffusion Sensitivity Test was carried out. Finally ESBL enzymes were identified by Real Time Polymerase Chain Reaction (rPCR). The results revealed that, out of the 100 Enterobacteriacae, 17 isolates (17 %) were positive phenotypically for ESBL. Of which, 14 isolates were positive for genotypically for ESBL. Furthermore, it was found that CTX-M Gene is the most prevalent gene in the Enterobacteriacae isolates

Key words: Enterobacteriacae,antibiotic resistance ,


 
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Pubmed Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. 2018; 58(1): 102-108. doi:10.5455/ajvs.285225


Web Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. http://www.alexjvs.com/?mno=285225 [Access: August 19, 2018]. doi:10.5455/ajvs.285225


AMA (American Medical Association) Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. 2018; 58(1): 102-108. doi:10.5455/ajvs.285225



Vancouver/ICMJE Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS. (2018), [cited August 19, 2018]; 58(1): 102-108. doi:10.5455/ajvs.285225



Harvard Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky (2018) Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. AJVS, 58 (1), 102-108. doi:10.5455/ajvs.285225



Turabian Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. 2018. Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. Alexandria Journal of Veterinary Sciences, 58 (1), 102-108. doi:10.5455/ajvs.285225



Chicago Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. "Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk." Alexandria Journal of Veterinary Sciences 58 (2018), 102-108. doi:10.5455/ajvs.285225



MLA (The Modern Language Association) Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky. "Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk." Alexandria Journal of Veterinary Sciences 58.1 (2018), 102-108. Print. doi:10.5455/ajvs.285225



APA (American Psychological Association) Style

El Sayed F.H. Ibrahim| Ashraf M. Abd El wahab| Samy A. Khalil| Helmy A. Torky (2018) Prevalence of Esbl Producing Enterobacteriacae Isolated from Bovine Mastitis Milk. Alexandria Journal of Veterinary Sciences, 58 (1), 102-108. doi:10.5455/ajvs.285225





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