E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs

Asmaa Magouz, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, Abdelrazek Desouky.

Abstract
The aim of this study was the isolation, identification and molecular characterization of Avian (Pigeon) Paramyxovirus-1 (APMV-1) isolated from clinically affected pigeons suspected to be infected with PPMV-1 in Egypt between 2016 and 2017. Twenty-five field samples were collected and inoculated into the allantoic cavity of ECE. Allantoic fluids were tested for haemagglutinating (HA) activity followed by haemagglutination inhibition (HI) test for virus identification. Molecular confirmation was done by reverse transcription polymerase chain reaction (RT-PCR) using primers specific to the fusion (F) gene. Results revealed 12 out of 25 positive samples. Two samples were selected for sequencing and phylogenetic analysis which revealed that two different amino acid motifs were found at the cleavage site of F protein: 112 KRQKRF117 (associated with virulent strains) and 112GRQGRL117 (associated with lentogenic strains).To our knowledge, this is the first reported PPMV-1 isolates that possess the sequences of 112GRQGRL117 within the F0 protein.

Key words: Pigeon Paramyxovirus-1, F gene, Phylogenetic analysis, Amino acid motifs


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Asmaa Magouz
Articles by Ali Etman
Articles by Abdelnaby Metwally
Articles by Gabr Elbagoury
Articles by Abdelrazek Desouky
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. 2018; 59(1): 1-10. doi:10.5455/ajvs.302643191


Web Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. https://www.alexjvs.com/?mno=302643191 [Access: December 06, 2023]. doi:10.5455/ajvs.302643191


AMA (American Medical Association) Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. 2018; 59(1): 1-10. doi:10.5455/ajvs.302643191



Vancouver/ICMJE Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. (2018), [cited December 06, 2023]; 59(1): 1-10. doi:10.5455/ajvs.302643191



Harvard Style

Magouz, A., Etman, . A., Metwally, . A., Elbagoury, . G. & Desouky, . A. (2018) Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS, 59 (1), 1-10. doi:10.5455/ajvs.302643191



Turabian Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. 2018. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. Alexandria Journal of Veterinary Sciences, 59 (1), 1-10. doi:10.5455/ajvs.302643191



Chicago Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. "Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs." Alexandria Journal of Veterinary Sciences 59 (2018), 1-10. doi:10.5455/ajvs.302643191



MLA (The Modern Language Association) Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. "Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs." Alexandria Journal of Veterinary Sciences 59.1 (2018), 1-10. Print. doi:10.5455/ajvs.302643191



APA (American Psychological Association) Style

Magouz, A., Etman, . A., Metwally, . A., Elbagoury, . G. & Desouky, . A. (2018) Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. Alexandria Journal of Veterinary Sciences, 59 (1), 1-10. doi:10.5455/ajvs.302643191





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles