E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 89
89Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 26 Apr 2026
 


Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine

Fatma M. Gadallah, Sayed, M. L., Nermeen A. Marden, Maha M. Sayed, Ahmed M. Helal, Selim S. Salama.


Abstract
Haemorrhagic septicaemia (HS) is a severe septicemic disease affecting cattle and other ruminants, particularly in tropical and subtropical regions, causing significant economic losses. Vaccination remains the most effective control strategy, and several serological assays, including Indirect Haemagglutination Assay (IHA) are used to evaluate vaccine-induced antibody responses.
This study assessed rabbits as an alternative model for evaluating the potency of Pasteurella multocida vaccines using the IHA test and investigated its correlation with LD50 induced in mice. Ten vaccine batches were evaluated using calves, New Zealand rabbits and swiss mice. Post-vaccination antibody titers showed strong immune responses in both species, with higher titers associated with increased protection in the mouse challenge test. A positive correlation between IHA titers and LD50 values was observed.
These findings suggest that the IHA assay in rabbits may provide a reliable and ethical alternative way for vaccine potency testing, reducing dependence on traditional mouse challenge assays.

Key words: Pasteurella multocida, Haemorrhagic septicaemia, ruminant, vaccine evaluation, rabbit as alternative model


 
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How to Cite this Article
Pubmed Style

Gadallah FM, Sayed , L. M, Marden NA, Sayed MM, Helal AM, Salama SS. Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. AJVS. Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969

Web Style

Gadallah FM, Sayed , L. M, Marden NA, Sayed MM, Helal AM, Salama SS. Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. https://www.alexjvs.com/?mno=313969 [Access: June 28, 2026]. doi:10.5455/ajvs.313969

AMA (American Medical Association) Style

Gadallah FM, Sayed , L. M, Marden NA, Sayed MM, Helal AM, Salama SS. Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. AJVS. Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969


Vancouver/ICMJE Style

Gadallah FM, Sayed , L. M, Marden NA, Sayed MM, Helal AM, Salama SS. Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. AJVS, [cited June 28, 2026]; Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969


Harvard Style

Gadallah, F. M., Sayed, ., L., . M., Marden, . N. A., Sayed, . M. M., Helal, . A. M. & Salama, . S. S. (2026) Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. AJVS, Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969


Turabian Style

Gadallah, Fatma M., Sayed, M. L., Nermeen A. Marden, Maha M. Sayed, Ahmed M. Helal, and Selim S. Salama. 2026. Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. Alexandria Journal of Veterinary Sciences, Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969


Chicago Style

Gadallah, Fatma M., Sayed, M. L., Nermeen A. Marden, Maha M. Sayed, Ahmed M. Helal, and Selim S. Salama. "Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine." Alexandria Journal of Veterinary Sciences Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969


MLA (The Modern Language Association) Style

Gadallah, Fatma M., Sayed, M. L., Nermeen A. Marden, Maha M. Sayed, Ahmed M. Helal, and Selim S. Salama. "Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine." Alexandria Journal of Veterinary Sciences Online First: 26 Apr, 2026. Web. 28 Jun 2026 doi:10.5455/ajvs.313969


APA (American Psychological Association) Style

Gadallah, F. M., Sayed, ., L., . M., Marden, . N. A., Sayed, . M. M., Helal, . A. M. & Salama, . S. S. (2026) Using of rabbits as alternative lab animal for the evaluation of ruminant pasteurella multocida vaccine. Alexandria Journal of Veterinary Sciences, Online First: 26 Apr, 2026. doi:10.5455/ajvs.313969




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Alexandria University
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    Abees 10th district, Alexandria
    Egypt.

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