E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 89
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Original Article
Online Published: 28 Jun 2026
 


Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model

Asmaa T. Talayea, Mariam M. El-shemy, Amr A. El-saadany, Soad Belih, Alaa N. Elsharkawy, Yasmine A. Shahine, Samr H. Kassem.


Abstract
In recent years, significant research efforts have focused on developing antibacterial agents derived from natural products, especially those formulated at the nanoscale. This work study antibacterial effect of Curcumin Nano-emulsion (Cur-NE) against Staphylococcus aureus (S.aureus) isolated from clinical bovine mastitis, both in vitro and in vivo assessment studies were used. Firstly, S.aureus was isolated with a total rate of 28% using antimicrobial susceptibility test showed multidrug resistance against oxacillin, amoxicillin/clavulanic acid and tetracycline. Minimal inhibitory and bactericidal concentrations were 150 μg/ml and 300 μg/ml respectively. Using of 150 μg/ml of Cur-NE in combination with different antimicrobials by applying the agar well diffusion technique enhance the inhibition activity of gentamicin and norfloxacin However, it slightly suppressed the activity of amoxicillin/clavulanic acid, Oxacillin and tetracycline, by using Transmission electron microscope (TEM) Cur-NE lead to destruction of cell wall and leakage of cellular contents of bacterial cell. Then, a total of 25 lactating rabbits were grouped to 5 groups (5 each), G1: negative-control, G2: infected-control, G3: gentamicin treated group, G4: Cur-NE treated group and G5: gentamicin + Cur-NE treated group after the end of treatment, re isolation of S.aureus ,gene expression of TNF-α and IL-6 in infected tissue and histopathological examination were investigated. Our result revealed that Cur-NE has a potent antibacterial effect on S.aureus and can significantly decrease the expression of TNF-α and IL-6 genes with a greater noticeable effect for its combination with gentamicin. Histopathological findings revealed that S.aureus induced severe, toxin-mediated mastitis, while treatment with gentamicin and Cur-NE especially in combination significantly improved outcomes. Cur-NE showed enhanced antibacterial and anti-inflammatory effects, highlighting its potential as an effective adjunct therapy

Key words: Curcumin Nano-emulsion, S.aureus, Mastitis, TEM, TNF-αand IL-6


 
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How to Cite this Article
Pubmed Style

Talayea AT, El-shemy MM, El-saadany AA, Belih S, Elsharkawy AN, Shahine YA, Kassem SH. Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. AJVS. Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138

Web Style

Talayea AT, El-shemy MM, El-saadany AA, Belih S, Elsharkawy AN, Shahine YA, Kassem SH. Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. https://www.alexjvs.com/?mno=318138 [Access: July 04, 2026]. doi:10.5455/ajvs.318138

AMA (American Medical Association) Style

Talayea AT, El-shemy MM, El-saadany AA, Belih S, Elsharkawy AN, Shahine YA, Kassem SH. Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. AJVS. Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138


Vancouver/ICMJE Style

Talayea AT, El-shemy MM, El-saadany AA, Belih S, Elsharkawy AN, Shahine YA, Kassem SH. Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. AJVS, [cited July 04, 2026]; Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138


Harvard Style

Talayea, A. T., El-shemy, . M. M., El-saadany, . A. A., Belih, . S., Elsharkawy, . A. N., Shahine, . Y. A. & Kassem, . S. H. (0) Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. AJVS, Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138


Turabian Style

Talayea, Asmaa T., Mariam M. El-shemy, Amr A. El-saadany, Soad Belih, Alaa N. Elsharkawy, Yasmine A. Shahine, and Samr H. Kassem. 0. Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. Alexandria Journal of Veterinary Sciences, Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138


Chicago Style

Talayea, Asmaa T., Mariam M. El-shemy, Amr A. El-saadany, Soad Belih, Alaa N. Elsharkawy, Yasmine A. Shahine, and Samr H. Kassem. "Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model." Alexandria Journal of Veterinary Sciences Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138


MLA (The Modern Language Association) Style

Talayea, Asmaa T., Mariam M. El-shemy, Amr A. El-saadany, Soad Belih, Alaa N. Elsharkawy, Yasmine A. Shahine, and Samr H. Kassem. "Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model." Alexandria Journal of Veterinary Sciences Online First: 28 Jun, 2026. Web. 04 Jul 2026 doi:10.5455/ajvs.318138


APA (American Psychological Association) Style

Talayea, A. T., El-shemy, . M. M., El-saadany, . A. A., Belih, . S., Elsharkawy, . A. N., Shahine, . Y. A. & Kassem, . S. H. (0) Antimicrobial Potential of Curcumin Nano-emulsion Alone and in Combination Therapy Against Multidrug-Resistant Staphylococcus aureus Induced Mastitis in Rabbit Model. Alexandria Journal of Veterinary Sciences, Online First: 28 Jun, 2026. doi:10.5455/ajvs.318138




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