Abstract
This study was carried out to determine the difference in biochemical parameters and hematological picture between smooth inactive ovaries (SIO) and normal cyclic buffalo-cows. The study was carried out on forty adult Buffalo-cows with ages ranging from 4-7 years in different cities of Menofiya Governorate during the period from September 2014 till September 2015. Animals were classified into cyclic or non-cyclic based on gynecological examination by rectal palpation and trans-rectal ultrasonography. The first group was consisted of thirty female buffaloes affected with smooth inactive ovaries (SIO) and the second group was consisted of ten normal cyclic Buffalo-cows (C). Blood samples were collected for biochemical and hematological studies of both groups. The biochemical parameters revealed that, there was a significant decrease in RBCs (7.44±0.21 and 5.19±0.27), Hb (14.37±0.31 and 11.17±0.27) and PCV (43.11±0.78 and 31.33±2.52), while there was a significant increase in TLC (6.46±0.56 and 10.34±0.87), neutrophil (3.49±0.42 and 5.42±1.25), eosinophil (0.18±0.14 and 0.61±0.08) and lymphocyte (2.41±0.35 and 3.85±0.89) in normal cyclic and SIO groups respectively. While the hematological picture revealed that, there was a significant decrease in total protein (7.33±0.64 and 5.28 ± 0.20), albumin (4.42±0.37 and 3.20±0.24), globulin (2.88±0.28 and 2.08±0.34), glucose (102.42±7.45 and 53.03±8.47), creatinine (1.67±0.14 and 1.24±0.06), calcium (11.27±1.04 and 6.54±0.63) and inorganic phosphorous (4.77±0.45 and 2.76±0.20) in normal cyclic and SIO groups respectively. Also, the hormonal assay revealed that, there was a significant decrease in FSH (4.32±0.21 and 1.85 ± 0.02) and progesterone (2.54±0.88 and 1.72±0.34) in normal cyclic and SIO groups respectively. So that the cases of smooth inactive ovaries in buffalo-cows were characterized by an increase in TLC, neutrophil, eosinophil and lymphocyte and a decrease in RBCs, Hb, PCV, total protein, albumin, globulin, glucose, creatinine, calcium, inorganic phosphorous, FSH and progesterone.

Key words: Biochemical, hematological, smooth ovaries, buffalo-cows