E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis

Rasha T ElFaramaway, Eman E Abdeen, Ashraf A Abdel Tawab, Walid S mousa.

Abstract
Mastitis is a common global and economic dairy infection in cattle causing significant financial losses and responsible for inferior milk quality and increase the treatment costs as well as act as source for new intermammary infection. Staphylococcus aureus is recognized as a problematic bacteria affecting dairy herds. This study spot highlight on the antibiogram pattern with molecular characterization of virulence genes (coa and spa) of methicillin resistant S. aureus (MRSA) from clinical mastitic cases. From examined 400 cows of small holders 120 (30%) showed clinical mastitis and S. aureus prevalence rate was 46.5%. Higher resistance to Penicillin, Chloramphenicol, Oxacillin, Tetracycline and Erythromycin were showed in antibiogram testing, while high sensitivity to Gentamycin, Vancomycin, Amoxicillin/ clavunate acid and Ciprofloxacin were observed. Moreover, out of 46 S. aureus isolates, 31(67.39%) identified as MRSA strains of highly resistance to different antimicrobials classes. The PCR model was sensitive and rapid in identification of coa and spa genes in all examined strains at 630 and 229 bp respectively. In conclusion, S. aureus represented a common pathogen causing mastitis and exhibit resistant to different antibiotics groups, in addition to it carried virulence factors such as coa and spa which play a valuable effect in the disease severity.

Key words: antibiogram, S.aureus, mastitis , PCR, coa, spa


 
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How to Cite this Article
Pubmed Style

ElFaramaway RT, Abdeen EE, AAAT, mousa WS, . Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. AJVS. 2019; 61(1): 32-38. doi:10.5455/ajvs.41507


Web Style

ElFaramaway RT, Abdeen EE, AAAT, mousa WS, . Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. http://www.alexjvs.com/?mno=41507 [Access: February 09, 2020]. doi:10.5455/ajvs.41507


AMA (American Medical Association) Style

ElFaramaway RT, Abdeen EE, AAAT, mousa WS, . Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. AJVS. 2019; 61(1): 32-38. doi:10.5455/ajvs.41507



Vancouver/ICMJE Style

ElFaramaway RT, Abdeen EE, AAAT, mousa WS, . Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. AJVS. (2019), [cited February 09, 2020]; 61(1): 32-38. doi:10.5455/ajvs.41507



Harvard Style

ElFaramaway, R. T., Abdeen, E. E., , A. A. A. T., mousa, W. S. & (2019) Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. AJVS, 61 (1), 32-38. doi:10.5455/ajvs.41507



Turabian Style

ElFaramaway, Rasha T, Eman E Abdeen, Ashraf A Abdel Tawab, Walid S mousa, and . 2019. Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. Alexandria Journal of Veterinary Sciences, 61 (1), 32-38. doi:10.5455/ajvs.41507



Chicago Style

ElFaramaway, Rasha T, Eman E Abdeen, Ashraf A Abdel Tawab, Walid S mousa, and . "Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis." Alexandria Journal of Veterinary Sciences 61 (2019), 32-38. doi:10.5455/ajvs.41507



MLA (The Modern Language Association) Style

ElFaramaway, Rasha T, Eman E Abdeen, Ashraf A Abdel Tawab, Walid S mousa, and . "Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis." Alexandria Journal of Veterinary Sciences 61.1 (2019), 32-38. Print. doi:10.5455/ajvs.41507



APA (American Psychological Association) Style

ElFaramaway, R. T., Abdeen, E. E., , A. A. A. T., mousa, W. S. & (2019) Antibiogram Profile and Molecular Characterization of coa and spa Genes Of Methicillin Resistant Staphylococcus aureus (MRSA) from Clinical Mastitis. Alexandria Journal of Veterinary Sciences, 61 (1), 32-38. doi:10.5455/ajvs.41507





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