E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis

Eman S. Ramadan, Amal H. Ali, Lamees A. El-Tantawy, Ibrahim G. Ibrahim.

Abstract
To address the effect of serum treatment with chloroform (STC) on increasing the specificity of the Rose Bengal test (RBT), 200 serum samples (n=100 buffaloes and n=100 cows) were tested. For each animal species, 50 were apparently healthy and 50 were aborted. RBT and indirect enzyme-linked immunosorbent assay (iELISA) were applied for testing the serum before and after treatment with chloroform. The coherence between tests was poor (K=0.004, SE=0,003), (K=0.031, SE=0.24) and (K=0.176, SE=0,143) for apparently healthy buffaloes, apparently healthy cows and aborted buffaloes respectively, but it was only fair (K=0.386, SE=0,185) for aborted cows. After STC, the coherence between tests was markedly improved, where it became perfect (K=1.0, SE=0.0), (K=0.834, SE=0.113) and very good (K=0.896, SE=0.071) for apparently healthy buffaloes and cows, aborted cows and aborted buffaloes. The specificity and sensitivity of RBT were obviously raised after STC recording (100% and 100%), (80% and 100%) and (75% and 100%) for apparently healthy buffaloes and cows, aborted buffaloes and aborted cows respectively. Current results recommended that STC is a reliable method to avoid false positives. The higher specificity and sensitivity obtained by RBT after using STC suggests that it is not only can be used as a rapid, simple, economical and convenient screening method but also it can be used as a confirmatory test for serodiagnosis of brucellosis for large scale in endemic areas.

Key words: Brucellosis, Chloroform, RBT, Serum treatment, Specificity.


 
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How to Cite this Article
Pubmed Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. 2019; 61(2): 32-37. doi:10.5455/ajvs.47618


Web Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. http://www.alexjvs.com/?mno=47618 [Access: July 23, 2019]. doi:10.5455/ajvs.47618


AMA (American Medical Association) Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. 2019; 61(2): 32-37. doi:10.5455/ajvs.47618



Vancouver/ICMJE Style

Ramadan ES, Ali AH, El-Tantawy LA, Ibrahim IG. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS. (2019), [cited July 23, 2019]; 61(2): 32-37. doi:10.5455/ajvs.47618



Harvard Style

Ramadan, E. S., Ali, . A. H., El-Tantawy, . L. A. & Ibrahim, . I. G. (2019) Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. AJVS, 61 (2), 32-37. doi:10.5455/ajvs.47618



Turabian Style

Ramadan, Eman S., Amal H. Ali, Lamees A. El-Tantawy, and Ibrahim G. Ibrahim. 2019. Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. Alexandria Journal of Veterinary Sciences, 61 (2), 32-37. doi:10.5455/ajvs.47618



Chicago Style

Ramadan, Eman S., Amal H. Ali, Lamees A. El-Tantawy, and Ibrahim G. Ibrahim. "Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis." Alexandria Journal of Veterinary Sciences 61 (2019), 32-37. doi:10.5455/ajvs.47618



MLA (The Modern Language Association) Style

Ramadan, Eman S., Amal H. Ali, Lamees A. El-Tantawy, and Ibrahim G. Ibrahim. "Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis." Alexandria Journal of Veterinary Sciences 61.2 (2019), 32-37. Print. doi:10.5455/ajvs.47618



APA (American Psychological Association) Style

Ramadan, E. S., Ali, . A. H., El-Tantawy, . L. A. & Ibrahim, . I. G. (2019) Effect of Serum Treatment with Chloroform on Increasing Specificity of Rose Bengal Test for Diagnosis of Brucellosis. Alexandria Journal of Veterinary Sciences, 61 (2), 32-37. doi:10.5455/ajvs.47618





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