E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources

Ahmed L. El-Ashmony, Alaa El-din Mostafa, Reda Tarabees.

Abstract
The aim of the current study was to characterize E. coli isolates belonging to several serotypes in samples from different animal sources. The genetic diversity among the isolates was evaluated by multiplex PCR. Out of 30 suspected isolated E. coli which were identified microscopically, culturally and biochemically examined by VITEK2 and serologically identified there were 8 isolates showing low discrimination for E. coli and were non-typeable while 22 isolates positive, showing excellent level of identification for E. coli and were identified serologically. Thirteen different serogroups were identified, O26:H11, O91:H21, O78, O111:H2, O125:H21, O146:H21, O1:H7, O44:H18, O55:H7, O119:H6, O121:H7, O128:H2 and O159. Findings of multiplex PCR of virulence genes showed that the eaeA gene was successfully amplified in O26:H11; stx1 gene was successfully amplified in O26:H11, O55:H7, O91:H21, O111:H2, O119:H6, O128:H2 and O146:H21 serogroups but stx2 gene was successfully amplified in O1:H7, O26:H11, O78, O91:H21, O111:H2, O119:H6 and O125:H21. O91:H21, O111:H2 and O119:H6 serogroups carried both (stx1 and stx 2) genes, O26:H11 serogroup carried (stx 1, stx 2 and eae) genes and O44:H18, O121:H7 and O159 serogroups were negative for these genes. Findings of multiplex PCR of β-lactamase antimicrobial resistance genes showed that blaOXA gene was successfully amplified in O26:H11 only; blaCTX-M1 gene was successfully amplified in O78 and O121:H7 serogroups but blaTEM gene was successfully amplified in O26:H11, O91:H21, O111:H2 and O125: H21. O26:H11 serogroup carried both (blaOXA and blaTEM) genes and O1: H7, O44:H18, O55:H7, O119:H6, O128:H2, O146:H21 and O159 serogroups were negative for (blaOXA, blaCTX-M and blaTEM) genes. In conclusion, animals and animal products could be a significant reservoir of diverse E. coli virulence and antimicrobial resistance genes which is potentially hazardous to public health.

Key words: E. coli, VITEK2, serotyping, PCR, virulence genes, β-lactamase resistance genes.


 
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How to Cite this Article
Pubmed Style

El-Ashmony AL, Mostafa AE, Tarabees R. Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. AJVS. 2022; 74(2): 6-15. doi:10.5455/ajvs.75056


Web Style

El-Ashmony AL, Mostafa AE, Tarabees R. Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. https://www.alexjvs.com/?mno=75056 [Access: September 01, 2022]. doi:10.5455/ajvs.75056


AMA (American Medical Association) Style

El-Ashmony AL, Mostafa AE, Tarabees R. Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. AJVS. 2022; 74(2): 6-15. doi:10.5455/ajvs.75056



Vancouver/ICMJE Style

El-Ashmony AL, Mostafa AE, Tarabees R. Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. AJVS. (2022), [cited September 01, 2022]; 74(2): 6-15. doi:10.5455/ajvs.75056



Harvard Style

El-Ashmony, A. L., Mostafa, . A. E. & Tarabees, . R. (2022) Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. AJVS, 74 (2), 6-15. doi:10.5455/ajvs.75056



Turabian Style

El-Ashmony, Ahmed L., Alaa El-din Mostafa, and Reda Tarabees. 2022. Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. Alexandria Journal of Veterinary Sciences, 74 (2), 6-15. doi:10.5455/ajvs.75056



Chicago Style

El-Ashmony, Ahmed L., Alaa El-din Mostafa, and Reda Tarabees. "Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources." Alexandria Journal of Veterinary Sciences 74 (2022), 6-15. doi:10.5455/ajvs.75056



MLA (The Modern Language Association) Style

El-Ashmony, Ahmed L., Alaa El-din Mostafa, and Reda Tarabees. "Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources." Alexandria Journal of Veterinary Sciences 74.2 (2022), 6-15. Print. doi:10.5455/ajvs.75056



APA (American Psychological Association) Style

El-Ashmony, A. L., Mostafa, . A. E. & Tarabees, . R. (2022) Molecular Characterization of Virulence and Antimicrobial Resistance Genes of E. coli Isolated from Different Animal Sources. Alexandria Journal of Veterinary Sciences, 74 (2), 6-15. doi:10.5455/ajvs.75056





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