E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen

Muhammad M. Arboud, Reham S. Waheeb, Reda I. El-Sheshtawy, Gamal A. El-Amrawi.

Abstract
Preservation of semen either by cooling or freezing results in structural and functional damage of spermatozoa. Exogenous antioxidants are added to semen extender to counteract this damage and enhance its preservability. This study was designed to evaluate effect of adding various concentrations of Royal jelly to tris-extender on bull semen characteristics after cooling and post-freezing. Methods: Pooled bull semen were extended with tris-citrate-fructose egg yolk diluent (control: 0% Royal jelly) and various concentrations of Royal jelly (RJ: 0.05%, RJ2: 0.1%, RJ3: 0.2%, RJ4: 0.3%). Extended semen was subjected slow cooling for 4 hrs, followed by freezing. Cooled and post-thawed semen was assessed for percentage of motility, alive, abnormality, membrane integrity, and acrosome morphology. Additionally, post-thawed semen was used for artificial insemination to assess its field fertility.
Results: In cooled semen, sperm motility percentage in the four concentrations of royal jelly was comparable to the control. Percentage of alive sperm was superior in the first concentration (RJ1). The second, third and the fourth concentrations (RJ2, RJ3 and RJ4) gave the best HOST percentage relative to the control. Acrosome integrity was the highest in the second concentration (RJ2), while the lowest was at the fourth concentration (RJ4) as compared to the control. Motility and percentage of alive spermatozoa in post-thawed semen was superior in (RJ1, RJ2) concentrations of royal jelly relative to the control. Also, sperm abnormalities were significantly reduced in (RJ1, RJ2). The HOST percentage was significantly higher in the fourth concentration (RJ4). Acrosome integrity was significantly higher in all concentrations and the best was in (RJ1, RJ2) compared to the control and other concentrations. Conception rate was the best in RJ1and RJ2. Conclusions: Addition of royal jelly to bull semen extender improved its quality during cooling and post-thawing.

Key words: Royal jelly, bull, semen quality, extender


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Muhammad M. Arboud
Articles by Reham S. Waheeb
Articles by Reda I. El-Sheshtawy
Articles by Gamal A. El-Amrawi
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Arboud MM, Waheeb RS, El-Sheshtawy RI, El-Amrawi GA. Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. AJVS. 2021; 68(1): 107-112. doi:10.5455/ajvs.86273


Web Style

Arboud MM, Waheeb RS, El-Sheshtawy RI, El-Amrawi GA. Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. http://www.alexjvs.com/?mno=86273 [Access: February 26, 2021]. doi:10.5455/ajvs.86273


AMA (American Medical Association) Style

Arboud MM, Waheeb RS, El-Sheshtawy RI, El-Amrawi GA. Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. AJVS. 2021; 68(1): 107-112. doi:10.5455/ajvs.86273



Vancouver/ICMJE Style

Arboud MM, Waheeb RS, El-Sheshtawy RI, El-Amrawi GA. Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. AJVS. (2021), [cited February 26, 2021]; 68(1): 107-112. doi:10.5455/ajvs.86273



Harvard Style

Arboud, M. M., Waheeb, . R. S., El-Sheshtawy, . R. I. & El-Amrawi, . G. A. (2021) Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. AJVS, 68 (1), 107-112. doi:10.5455/ajvs.86273



Turabian Style

Arboud, Muhammad M., Reham S. Waheeb, Reda I. El-Sheshtawy, and Gamal A. El-Amrawi. 2021. Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. Alexandria Journal of Veterinary Sciences, 68 (1), 107-112. doi:10.5455/ajvs.86273



Chicago Style

Arboud, Muhammad M., Reham S. Waheeb, Reda I. El-Sheshtawy, and Gamal A. El-Amrawi. "Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen." Alexandria Journal of Veterinary Sciences 68 (2021), 107-112. doi:10.5455/ajvs.86273



MLA (The Modern Language Association) Style

Arboud, Muhammad M., Reham S. Waheeb, Reda I. El-Sheshtawy, and Gamal A. El-Amrawi. "Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen." Alexandria Journal of Veterinary Sciences 68.1 (2021), 107-112. Print. doi:10.5455/ajvs.86273



APA (American Psychological Association) Style

Arboud, M. M., Waheeb, . R. S., El-Sheshtawy, . R. I. & El-Amrawi, . G. A. (2021) Effect of Different Concentrations of Tris Royal Jelly-Enriched Extender on Cooled and Post-Thawed Bull Semen. Alexandria Journal of Veterinary Sciences, 68 (1), 107-112. doi:10.5455/ajvs.86273





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]