E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Some Studies on E-Coli Mastitis in Cattle and Buffaloes

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail.

Abstract
The aim of this study is to identify the role of E.coli in bovine mastitis, its virulence factors and antimicrobial sensitivity. 360 lactating cows and buffaloes were examined clinically and by CMT, 210 were suffering from clinical mastitis with percentage of 62.7% and 39 (20.36%) E.coli isolates were detected in clinical cases of mastitis, 90 cases were suffering from subclinical mastitis and 6 (6.8%) E.coli isolates were detected in them. The percentage of E.coli in cows was 31 (21.142%) and in buffaloes 8 (18.33%) isolates. Serotyping of E.coli revealed that O55 (30%), O111 (15%), O124 (15%), O119 (10%), O114 (10%), O26 (5%), O157 (10%) and O44 (5%). That’s showing that O55, O111 and O124 the most serotypes causing mastitis. PCR identification of TraT gene revealed 5 cases were positive and give positive reaction at 307 bp. and for eaeA gene revealed 6 cases and give positive reaction at 384 bp. The antimicrobial sensitivity indicated that the most effective antibiotics were lincospectine (56.6%), danofloxacin (56.6%), enrofloxacin (40%) and ceftifure (40%), while the lowest effective antibiotics were oxytetracycline and ampicillin.

Key words: E.coli, Cattle, Buffaloes, Serological identification, PCR


 
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How to Cite this Article
Pubmed Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. 2015; 45(1): 105-112. doi:10.5455/ajvs.178113


Web Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. https://www.alexjvs.com/?mno=178113 [Access: December 03, 2023]. doi:10.5455/ajvs.178113


AMA (American Medical Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. 2015; 45(1): 105-112. doi:10.5455/ajvs.178113



Vancouver/ICMJE Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. (2015), [cited December 03, 2023]; 45(1): 105-112. doi:10.5455/ajvs.178113



Harvard Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail (2015) Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS, 45 (1), 105-112. doi:10.5455/ajvs.178113



Turabian Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. 2015. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. Alexandria Journal of Veterinary Sciences, 45 (1), 105-112. doi:10.5455/ajvs.178113



Chicago Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. "Some Studies on E-Coli Mastitis in Cattle and Buffaloes." Alexandria Journal of Veterinary Sciences 45 (2015), 105-112. doi:10.5455/ajvs.178113



MLA (The Modern Language Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. "Some Studies on E-Coli Mastitis in Cattle and Buffaloes." Alexandria Journal of Veterinary Sciences 45.1 (2015), 105-112. Print. doi:10.5455/ajvs.178113



APA (American Psychological Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail (2015) Some Studies on E-Coli Mastitis in Cattle and Buffaloes. Alexandria Journal of Veterinary Sciences, 45 (1), 105-112. doi:10.5455/ajvs.178113





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