E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology

Mohamed, A. Nossair and Yasser N. Haggag.

Abstract
A total of 300 human serum samples were examined by RBPT and c. ELISA to estimate the prevalence of brucellosis in humans in Alexandria Province and to investigate the epidemiological pattern of such disease among humans. It was found that the overall seroprevalence of brucellosis in humans through RBPT and c. ELISA was 14.67, and 11.67%, respectively. Statistical analysis showed that there was a significant association at (P<0.0001) between the seroprevalence of brucellosis among human beings in relation to place of residence where the highest prevalence was noticed in El- Aameria (18.40%) followed by Gharb (17.86%), Borg El Arab (16.66%), Sharq (13.33%), El Montazah (13.24%) and Wasat (12.5%). On contrary, all the examined samples that were collected from El- Gomrok were found to be negative. In addition, the results clarified that 25% of the examined samples collected from patients complaining of fever were found to be positive for brucellosis. On the other side, only 7.78% of the examined samples of apparently healthy individuals were found to be positive for brucellosis with significant association. Sex based seroprevalence showed that males' prevalence (16.67%) was higher than that in female (12.12%) with significant association at (P < 0.05). Also, age based seroprevalence showed that the highest prevalence was observed in the age group (20 - ˂ 40 years) (19.75%) followed by the age group (˂ 20 years) (13.48%) then the age group (40 - ˂ 60 years) (12.79%) and lastly the age group (˃ 60 years) (11.36%) with non-significant association between different age groups and prevalence of human brucellosis with significant association at (P < 0.01). Moreover, the highest seroprevalence was observed in farmers (16.30%) followed by housewives (14.25%), animals' attendants (14.10%), veterinarians (11.11%), slaughterhouse workers (8.33%) and finally veterinary students (0.59%) with significant association at (P < 0.01) between different occupations and prevalence of human brucellosis. Under the conditions in the current study and according to the data obtained, it is concluded that brucellosis is still remaining a problem in Alexandria province and it was threatening human population through direct and indirect transmission. Also, the prevalence of the disease is significantly associated with health status, sex, age and occupation while it is non-significantly associated with manner of milk consumption, locality and the type of animal contact.

Key words: Brucellosis, Human, seroprevalence, RBPT, c. ELISA


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed
Articles by A. Nossair and Yasser N. Haggag
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. 2016; 51(2): 296-302. doi:10.5455/ajvs.239565


Web Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. https://www.alexjvs.com/?mno=239565 [Access: December 07, 2023]. doi:10.5455/ajvs.239565


AMA (American Medical Association) Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. 2016; 51(2): 296-302. doi:10.5455/ajvs.239565



Vancouver/ICMJE Style

Mohamed, A. Nossair and Yasser N. Haggag. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS. (2016), [cited December 07, 2023]; 51(2): 296-302. doi:10.5455/ajvs.239565



Harvard Style

Mohamed, A. Nossair and Yasser N. Haggag (2016) Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. AJVS, 51 (2), 296-302. doi:10.5455/ajvs.239565



Turabian Style

Mohamed, A. Nossair and Yasser N. Haggag. 2016. Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. Alexandria Journal of Veterinary Sciences, 51 (2), 296-302. doi:10.5455/ajvs.239565



Chicago Style

Mohamed, A. Nossair and Yasser N. Haggag. "Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology." Alexandria Journal of Veterinary Sciences 51 (2016), 296-302. doi:10.5455/ajvs.239565



MLA (The Modern Language Association) Style

Mohamed, A. Nossair and Yasser N. Haggag. "Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology." Alexandria Journal of Veterinary Sciences 51.2 (2016), 296-302. Print. doi:10.5455/ajvs.239565



APA (American Psychological Association) Style

Mohamed, A. Nossair and Yasser N. Haggag (2016) Using Competitive ELISA for Accurate Diagnosis of Brucellosis in Humans in Alexandria Province with Emphasis on the Disease Epidemiology. Alexandria Journal of Veterinary Sciences, 51 (2), 296-302. doi:10.5455/ajvs.239565





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles