E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis

Doaa Kirat.

Abstract
The present work was designed to study the effect of metabolic acidosis induced by ammonium chloride on the expression of sodium-bicarbonate cotransporter 1 (NBC1) and Monocarboxylate transporter 1 (MCT1) in rat kidney. Following the treatment of NH4Cl, blood pH and bicarbonate concentration were significantly decreased, while significant increase in chloride concentration was developed. Moreover, urine analysis revealed decrease in urine pH and significant increase in ammonia in acidotic rats. The protein expression of NBC1 and MCT1 was determined in the rat kidney by immunofluorescence confocal laser-scanning microscopy and Western blotting analyses. In the experimental group, the expression level of NBC1 was significantly increased in the renal proximal tubules, whereas, the expression of MCT1 was significantly decreased. Western blotting data confirmed the immunofluorescence confocal laser-scanning microscopy. In conclusion, metabolic acidosis causes adaptive increases in the basolateral NBC1 in the rat renal proximal tubule that are likely responsible for the increased ability of the proximal tubule to reabsorb filtered bicarbonates. Additionally, acidosis leads to decrease the MCT1 protein expression level in renal proximal tubule that may explain the increase in lactate excretion in the urine of acidotic rats due to the reduction in lactate co-transport.

Key words: Monocarboxylate transporter 1, Sodium- bicarbonate cotransporter 1, Ammonium Chloride, Kidney, Rats.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Doaa Kirat
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. 2016; 51(1): 61-67. doi:10.5455/ajvs.242177


Web Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. https://www.alexjvs.com/?mno=242177 [Access: December 03, 2023]. doi:10.5455/ajvs.242177


AMA (American Medical Association) Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. 2016; 51(1): 61-67. doi:10.5455/ajvs.242177



Vancouver/ICMJE Style

Doaa Kirat. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS. (2016), [cited December 03, 2023]; 51(1): 61-67. doi:10.5455/ajvs.242177



Harvard Style

Doaa Kirat (2016) The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. AJVS, 51 (1), 61-67. doi:10.5455/ajvs.242177



Turabian Style

Doaa Kirat. 2016. The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. Alexandria Journal of Veterinary Sciences, 51 (1), 61-67. doi:10.5455/ajvs.242177



Chicago Style

Doaa Kirat. "The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis." Alexandria Journal of Veterinary Sciences 51 (2016), 61-67. doi:10.5455/ajvs.242177



MLA (The Modern Language Association) Style

Doaa Kirat. "The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis." Alexandria Journal of Veterinary Sciences 51.1 (2016), 61-67. Print. doi:10.5455/ajvs.242177



APA (American Psychological Association) Style

Doaa Kirat (2016) The Sodium-Bicarbonate Cotransporter 1 (NBC1) and Monocarboxylate Transporter 1 (MCT1) in the Rat Kidney are Regulated in Response to Metabolic Acidosis. Alexandria Journal of Veterinary Sciences, 51 (1), 61-67. doi:10.5455/ajvs.242177





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles