E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani.

Abstract
The present work designed to estimate postmortem expression of mRNA of certain hepatic genes in rats in the presence or absence of postmortem heat stress using qRT-PCR technique in correlation to postmortem interval (PMI). Rats were sacrificed and divided into, control group (PMI=0 h), room temperature and heat stressed (41⁰C) groups. In control group, liver samples were collected immediately after death, while in the second and third groups, samples were collected at 1, 3 and 6 hours postmortem. The results showed significant reductions in pro-inflammatory gene transcripts (TNFα and IL-1β) at all-time points compared to control group with an inhibitory effect of postmortem heat stress on expression of TNFα at 1 and 6 h after death. Whereas postmortem expression of genes encode apoptosis was significantly increased at 3 and 6 h postmortem compared to control group. Postmortem heat stress reduced the hepatic expression of Bcl-2 and Caspase-3 at 3 and 6 h of PMI and increased the expression of Caspase-3 at 1 h after death compared to room temperature group. Meanwhile, postmortem hepatic mRNA expression of c-fos was significantly induced after death at 3 and 6 h PMI. Postmortem heat stress significantly reduced c-fos expression at 3 and 6 h after death. Thanatotranscriptome of genes encode inflammation, apoptosis as well as c-fos varied in their correlations to PMI. Additionally, the results showed disparities in the effect of postmortem heat stress according to the studied gene and time.

Key words: Thanatotranscriptome, postmortem, heat stress, apoptosis, inflammation


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Amal A. Halawa
Articles by Mohamed M. El-Adl
Articles by Basma H. Marghani
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. 2018; 57(2): 13-20. doi:10.5455/ajvs.297063


Web Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. https://www.alexjvs.com/?mno=297063 [Access: December 03, 2023]. doi:10.5455/ajvs.297063


AMA (American Medical Association) Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. 2018; 57(2): 13-20. doi:10.5455/ajvs.297063



Vancouver/ICMJE Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS. (2018), [cited December 03, 2023]; 57(2): 13-20. doi:10.5455/ajvs.297063



Harvard Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani (2018) Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. AJVS, 57 (2), 13-20. doi:10.5455/ajvs.297063



Turabian Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. 2018. Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. Alexandria Journal of Veterinary Sciences, 57 (2), 13-20. doi:10.5455/ajvs.297063



Chicago Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. "Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress." Alexandria Journal of Veterinary Sciences 57 (2018), 13-20. doi:10.5455/ajvs.297063



MLA (The Modern Language Association) Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani. "Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress." Alexandria Journal of Veterinary Sciences 57.2 (2018), 13-20. Print. doi:10.5455/ajvs.297063



APA (American Psychological Association) Style

Amal A. Halawa, Mohamed M. El-Adl, Basma H. Marghani (2018) Thanatotranscriptome Study on Particular Hepatic Genes and Their Correlation With Postmortem Interval in the Presence or Absence of Postmortem Heat Stress. Alexandria Journal of Veterinary Sciences, 57 (2), 13-20. doi:10.5455/ajvs.297063





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles