E-ISSN 2536-9520 | ISSN 1110-2047
 

Short Communication 


Isolation of primary mice splenocytes for in vitro research

Fawzi O. Ebrahim, Abdul M. Abolayha, Adam A. Elzagheid, Fadella Abosrer.

SUPPLEMENTARY FILES :

  • Supplementary file - 1

  • Abstract
    Abstract
    Animal cell culture is of great importance in research. We isolated mouse splenocytes from Swiss albino mice by using a mechanical method and cultured them in medium with fetal bovine serum, 1% penicillin-streptomycin and 1% amphotericin as an antibiotic and an antifungal, but without mitogen. The cells were confluent after 72 h, and their viability, measured by trypan blue assay, was 95%. The splenocytes were passaged four times and no fungal or bacterial contamination was observed during carrying out the experiment.

    Key words: Key words: mouse spleen, splenocytes, trypan blue


     
    ARTICLE TOOLS
    Abstract
    PDF Fulltext
    How to cite this articleHow to cite this article
    Citation Tools
    Related Records
     Articles by Fawzi O. Ebrahim
    Articles by Abdul M. Abolayha
    Articles by Adam A. Elzagheid
    Articles by Fadella Abosrer
    on Google
    on Google Scholar


    How to Cite this Article
    Pubmed Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519


    Web Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. https://www.alexjvs.com/?mno=82519 [Access: June 28, 2026]. doi:10.5455/ajvs.82519


    AMA (American Medical Association) Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. 2020; 64(2): 13-20. doi:10.5455/ajvs.82519



    Vancouver/ICMJE Style

    Ebrahim FO, Abolayha AM, Elzagheid AA, Abosrer F. Isolation of primary mice splenocytes for in vitro research. AJVS. (2020), [cited June 28, 2026]; 64(2): 13-20. doi:10.5455/ajvs.82519



    Harvard Style

    Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. AJVS, 64 (2), 13-20. doi:10.5455/ajvs.82519



    Turabian Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. 2020. Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519



    Chicago Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. "Isolation of primary mice splenocytes for in vitro research." Alexandria Journal of Veterinary Sciences 64 (2020), 13-20. doi:10.5455/ajvs.82519



    MLA (The Modern Language Association) Style

    Ebrahim, Fawzi O., Abdul M. Abolayha, Adam A. Elzagheid, and Fadella Abosrer. "Isolation of primary mice splenocytes for in vitro research." Alexandria Journal of Veterinary Sciences 64.2 (2020), 13-20. Print. doi:10.5455/ajvs.82519



    APA (American Psychological Association) Style

    Ebrahim, F. O., Abolayha, . A. M., Elzagheid, . A. A. & Abosrer, . F. (2020) Isolation of primary mice splenocytes for in vitro research. Alexandria Journal of Veterinary Sciences, 64 (2), 13-20. doi:10.5455/ajvs.82519





    Most Viewed Articles
    Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290