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Original Article
Online Published: 30 Jun 2020
 


Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms

Amal M.a. Khalifa, Ghada A. El-gammal, Sabreen E. Fadl, Mohamed S. El-gohary, Mohamed E. Barakat.


Abstract
This work carried out to analyze the microbiology and physicochemical parameters of water in some Nile Tilapia fish farms in Kafrelsheikh Governorate. Total bacterial and total Coliforms count were analyzed, as well as the presence of E.faecalis, E.coli, and E.coli O157 in both fish and farms water. The level of total bacterial count in water varied between 4.3x104 and 1.3x106 CFU/ml. The (MPN) of total Coliforms ranged between <3 and >1100/ml between different sampling sites. E.faecalis was verified in 28% and 38% of fish and water samples respectively E.coli was isolated in 18% and 34% from fish water samples. E.coli O157 verified in 2% of fish samples and does not present in water samples. Sero-grouping of E.coli isolates revealed that E.coli belongs to five serogroups (O6, O27, O125, O126, and O157). Results of microbiology were confirmed by results of physicochemical parameters of the water samples where values of PH, conductivity, salinity, dissolved oxygen, ammonia, nitrate, nitrite, sulfate, chlorine, chloride, total solids, total suspended solids, and total dissolved solids were significantly (P<0.05) increased in the same farms, which contain high Coliform count.
Multiple antibiotic resistance was recorded in 18/28 E.faecalis isolates (64.3%) and in all E.coli isolates 20/20 (100%). Confirmatory diagnosis by polymerase chain reaction (PCR) using specific 16Sr RNA for E.faecalis with a product band (310 bp) showed that seven from the eight examined isolates were positive. In addition, detection of rfbE gene-specific for E.coli O157 with a product band (134 bp) confirmed in the two isolates.

Key words: Bacterial indicators, Chemical indicators, Fish farms, Water pollution, Kafrelsheikh Governorate.


 
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How to Cite this Article
Pubmed Style

Khalifa AM, El-gammal GA, Fadl SE, El-gohary MS, Barakat ME. Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. AJVS. 2020; 66(1): 76-88. doi:10.5455/ajvs.111726

Web Style

Khalifa AM, El-gammal GA, Fadl SE, El-gohary MS, Barakat ME. Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. https://www.alexjvs.com/?mno=111726 [Access: May 05, 2025]. doi:10.5455/ajvs.111726

AMA (American Medical Association) Style

Khalifa AM, El-gammal GA, Fadl SE, El-gohary MS, Barakat ME. Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. AJVS. 2020; 66(1): 76-88. doi:10.5455/ajvs.111726


Vancouver/ICMJE Style

Khalifa AM, El-gammal GA, Fadl SE, El-gohary MS, Barakat ME. Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. AJVS. (2020), [cited May 05, 2025]; 66(1): 76-88. doi:10.5455/ajvs.111726


Harvard Style

Khalifa, A. M., El-gammal, . G. A., Fadl, . S. E., El-gohary, . M. S. & Barakat, . M. E. (2020) Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. AJVS, 66 (1), 76-88. doi:10.5455/ajvs.111726


Turabian Style

Khalifa, Amal M.a., Ghada A. El-gammal, Sabreen E. Fadl, Mohamed S. El-gohary, and Mohamed E. Barakat. 2020. Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. Alexandria Journal of Veterinary Sciences, 66 (1), 76-88. doi:10.5455/ajvs.111726


Chicago Style

Khalifa, Amal M.a., Ghada A. El-gammal, Sabreen E. Fadl, Mohamed S. El-gohary, and Mohamed E. Barakat. "Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms." Alexandria Journal of Veterinary Sciences 66 (2020), 76-88. doi:10.5455/ajvs.111726


MLA (The Modern Language Association) Style

Khalifa, Amal M.a., Ghada A. El-gammal, Sabreen E. Fadl, Mohamed S. El-gohary, and Mohamed E. Barakat. "Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms." Alexandria Journal of Veterinary Sciences 66.1 (2020), 76-88. Print. doi:10.5455/ajvs.111726


APA (American Psychological Association) Style

Khalifa, A. M., El-gammal, . G. A., Fadl, . S. E., El-gohary, . M. S. & Barakat, . M. E. (2020) Laboratory Microbiological and Chemical Analysis for Detection of Water Pollution in Fresh Water Fish Farms. Alexandria Journal of Veterinary Sciences, 66 (1), 76-88. doi:10.5455/ajvs.111726




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