E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular characterization of vibrio species isolated from fish using PCR technique

Eslam A. Asran, Samy A. Khalil, Yasr N. Awad, Mona M. Hussein.

Abstract
A total of 100 fish samples (Oreochromis niloticus) were collected as 25 fish apparently health and 75 fish appeared to be clinically diseased fish. Samples of kidney, heart, liver, gills and skin lesions are collected and cultured on TCBS medium. Eighty three samples show biochemically positive to vibrio species.10 random samples amplified to 16srRNA gene by PCR technique for more accurate identification resulting in 8 isolates were positive to 16srRNA.The 8 isolates amplified to suspected species specific primers. Resulted in 4 V.parahaemolyticus (50%), 2 V. harveyi (25%) ,1 V. anguillarum (12.5%) and 1 V. alginolyticus (12.5%). Each species amplified to PCR technique for more identification to virulence genes. V.parahaemolyticus amplified to tdh and trh; V. harveyi to Partial hly and Vhh; V. alginolyticus amplified to trh and tdh and V.anguillarum amplified to virA and angM. This study proved that PCR protocols could be useful tools for rapid and simultaneous detection of different Vibrio species virulence factors. Also using of 16srRNA gene in molecular characterization (PCR) of Vibrio is the fastest and most accurate method to identify all isolates of Vibrio.

Key words: Vibriosis, vibrio species, PCR, 16srRNA and Virulence factors.


 
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How to Cite this Article
Pubmed Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. 2020; 67(2): 9-19. doi:10.5455/ajvs.117363


Web Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. http://www.alexjvs.com/?mno=117363 [Access: August 17, 2021]. doi:10.5455/ajvs.117363


AMA (American Medical Association) Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. 2020; 67(2): 9-19. doi:10.5455/ajvs.117363



Vancouver/ICMJE Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. (2020), [cited August 17, 2021]; 67(2): 9-19. doi:10.5455/ajvs.117363



Harvard Style

Asran, E. A., Khalil, . S. A., Awad, . Y. N. & Hussein, . M. M. (2020) Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS, 67 (2), 9-19. doi:10.5455/ajvs.117363



Turabian Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. 2020. Molecular characterization of vibrio species isolated from fish using PCR technique. Alexandria Journal of Veterinary Sciences, 67 (2), 9-19. doi:10.5455/ajvs.117363



Chicago Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. "Molecular characterization of vibrio species isolated from fish using PCR technique." Alexandria Journal of Veterinary Sciences 67 (2020), 9-19. doi:10.5455/ajvs.117363



MLA (The Modern Language Association) Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. "Molecular characterization of vibrio species isolated from fish using PCR technique." Alexandria Journal of Veterinary Sciences 67.2 (2020), 9-19. Print. doi:10.5455/ajvs.117363



APA (American Psychological Association) Style

Asran, E. A., Khalil, . S. A., Awad, . Y. N. & Hussein, . M. M. (2020) Molecular characterization of vibrio species isolated from fish using PCR technique. Alexandria Journal of Veterinary Sciences, 67 (2), 9-19. doi:10.5455/ajvs.117363





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