Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR

Esraa Fekry; Eman Abdeen; Youserya M. Hashem; Alaa El Din Husien Mostapha

doi:10.5455/ajvs.135548

2025, Vol: 86

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Original Article

AJVS. 2023; 76(1): 1-6

doi: 10.5455/ajvs.135548

Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR

Esraa Fekry, Eman Abdeen, Youserya M. Hashem, Alaa El Din Husien Mostapha.

Abstract
Mycoplasmosis is a major and economic threat currently facing the poultry industry worldwide. The main pathogenic mycoplasmas species are Mycoplasma gallisepticum and Mycoplasma synoviae. The aim of this study was to identify and molecular characterization of Mycoplasma species from broilers and breeder chickens. Two hundred samples were collected and cultured onto specific PPLO medium and then tested by real time PCR approaches. The results reported that 15 (7.5%) and 50 (25%) were positive by culture and PCR respectively. Among the positive samples, Mycoplasma synoviae was high prevalent 45 (90%) by real time PCR, and low prevalent 12(24%) detected by culture as well as Mycoplasma gallisepticum were positive 5 (10%) and 3(6%) with real time PCR and culture respectively. In conclusion, our study confirmed that the real time PCR was most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples than culture method.

Key words: M.gallisepticum, M. synoviae, culture, PCR, diagnosis

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Pubmed Style

Fekry E, Abdeen E, Hashem YM, Mostapha AEDH. Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. AJVS. 2023; 76(1): 1-6. doi:10.5455/ajvs.135548

Web Style

Fekry E, Abdeen E, Hashem YM, Mostapha AEDH. Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. https://www.alexjvs.com/?mno=135548 [Access: May 05, 2025]. doi:10.5455/ajvs.135548

AMA (American Medical Association) Style

Fekry E, Abdeen E, Hashem YM, Mostapha AEDH. Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. AJVS. 2023; 76(1): 1-6. doi:10.5455/ajvs.135548

Vancouver/ICMJE Style

Fekry E, Abdeen E, Hashem YM, Mostapha AEDH. Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. AJVS. (2023), [cited May 05, 2025]; 76(1): 1-6. doi:10.5455/ajvs.135548

Harvard Style

Fekry, E., Abdeen, . E., Hashem, . Y. M. & Mostapha, . A. E. D. H. (2023) Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. AJVS, 76 (1), 1-6. doi:10.5455/ajvs.135548

Turabian Style

Fekry, Esraa, Eman Abdeen, Youserya M. Hashem, and Alaa El Din Husien Mostapha. 2023. Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR. Alexandria Journal of Veterinary Sciences, 76 (1), 1-6. doi:10.5455/ajvs.135548

Chicago Style

Fekry, Esraa, Eman Abdeen, Youserya M. Hashem, and Alaa El Din Husien Mostapha. "Molecular Characterization of Mycoplasma Species Isolated from Broilers and Breeder Chickens by Real Time PCR." Alexandria Journal of Veterinary Sciences 76 (2023), 1-6. doi:10.5455/ajvs.135548

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APA (American Psychological Association) Style

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