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Original Article 


Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish

Mohammed Mohammed Mousa, Hany Mohammed Abo yousif, Walaa Ibrahium Ahmed, Asmaa Moataz El Shabacy.

Abstract
One hundred and eighty shellfish samples were collected from different fish and shellfish markets retailed at Alexandria province. The examined samples were analyzed for isolation and identification of different Vibrio species in 50 Shrimp sample, 50 Crab sample , 40 Squid sample,and 40 Octopus Vulgaris sample, then the positive identified V. parahemolyticus isolates were confirmed by Multiplex PCR for detection of pathogenic genes of V. parahemolyticus (TLH, RecA, TRH and TRH genes) , the samples that confirmed to be infected with V. parahemolyticus were subjected to test their ability for decarboxylation and production of Biogenic amine (Histamine and Putrescine) in vitro by Niven's test. Results showed that numbers and percentages of V. alginolyticus , V.parahemolyticus, V.cholera , and V.vulnificus isolates were 24 (13.3%) , 19(10.5%) , 17(9.4%) , and 9(5%) , respectively with overall incidence 69(38.3%) Vibrio isolates in all shellfish samples, then 19 positive genetically identified V. parahemolyticus isolates were confirmed to have 19 TLH and 18 RecA genes with total number of 37 gene (11 TLH , 10 RecA in shrimp , 6 TLH , 6 RecA in Crab and 2 TLH , 2 RecA in Squid) by Multiplex PCR, also 19 genetically identified V. parahemolyticus isolates were confirmed to decarboxylase histidine and ornithine to histamine and putrescine biogenic amines by using Niven's test.

Key words: Key words: shellfish, V. parahemolyticus, V.cholera , V.alginolyticus V.vulnificus , Histamine , Putrescine and Multiplex PCR.


 
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How to Cite this Article
Pubmed Style

Mousa MM, yousif HMA, Ahmed WI, Shabacy AME, . Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. AJVS. 2019; 61(2): 83-89. doi:10.5455/ajvs.14091

Web Style

Mousa MM, yousif HMA, Ahmed WI, Shabacy AME, . Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. https://www.alexjvs.com/?mno=14091 [Access: December 05, 2023]. doi:10.5455/ajvs.14091

AMA (American Medical Association) Style

Mousa MM, yousif HMA, Ahmed WI, Shabacy AME, . Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. AJVS. 2019; 61(2): 83-89. doi:10.5455/ajvs.14091


Vancouver/ICMJE Style

Mousa MM, yousif HMA, Ahmed WI, Shabacy AME, . Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. AJVS. (2019), [cited December 05, 2023]; 61(2): 83-89. doi:10.5455/ajvs.14091


Harvard Style

Mousa, M. M., yousif, . H. M. A., Ahmed, . W. I., Shabacy, . A. M. E. & (2019) Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. AJVS, 61 (2), 83-89. doi:10.5455/ajvs.14091


Turabian Style

Mousa, Mohammed Mohammed, Hany Mohammed Abo yousif, Walaa Ibrahium Ahmed, Asmaa Moataz El Shabacy, and . 2019. Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. Alexandria Journal of Veterinary Sciences, 61 (2), 83-89. doi:10.5455/ajvs.14091


Chicago Style

Mousa, Mohammed Mohammed, Hany Mohammed Abo yousif, Walaa Ibrahium Ahmed, Asmaa Moataz El Shabacy, and . "Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish." Alexandria Journal of Veterinary Sciences 61 (2019), 83-89. doi:10.5455/ajvs.14091


MLA (The Modern Language Association) Style

Mousa, Mohammed Mohammed, Hany Mohammed Abo yousif, Walaa Ibrahium Ahmed, Asmaa Moataz El Shabacy, and . "Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish." Alexandria Journal of Veterinary Sciences 61.2 (2019), 83-89. Print. doi:10.5455/ajvs.14091


APA (American Psychological Association) Style

Mousa, M. M., yousif, . H. M. A., Ahmed, . W. I., Shabacy, . A. M. E. & (2019) Genetic Diversity of Vibrio Parahemolyticus Isolated from some Shellfish. Alexandria Journal of Veterinary Sciences, 61 (2), 83-89. doi:10.5455/ajvs.14091




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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