E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants

Dina E. Ahmed, Iman E. El-Bawab, Gamal A. EL-Amrawi.

Abstract
The current study aimed to evaluate the impact of different antioxidants on the sperm quality-related parameters of frozen-thawed Brown Swiss bulls. Semen samples were collected from each bull once per week using an artificial vagina. The semen samples were pooled and divided into aliquots. One of them was diluted with a tris-based extender without any antioxidants and served as a control group. Other aliquots were diluted with a tris-based extender supplemented with 0.75% green tea extract, 0.25% strawberry extract, 1.5 mM of vitamin E, 2 mM of vitamin C, and 0.5 mM of caffeine at 37°C. The semen was evaluated for motility, viability, abnormality, plasma membrane integrity, and acrosome integrity immediately following the addition of diluent for the control group and again after one week of storage in liquid nitrogen at -196°C and re-thawing for all groups. The results revealed that green tea extract had the most beneficial impact on improving sperm parameters (motility, viability, abnormality, plasma membrane integrity, and acrosome integrity) followed by strawberry extract and vitamin E then vitamin C and caffeine as compared to the post-thawing control group. So, according to the results obtained, it was concluded that addition of green tea extract in the semen extender can enhance the quality of frozen-thawed bull sperm. However, given the price and availability of strawberry, we recommend using strawberry extract to boost seminal quality.

Key words: Antioxidants, Brown Swiss, Semen Cryopreservation, sperm parameters, semen quality, vitamins


 
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How to Cite this Article
Pubmed Style

Ahmed DE, El-Bawab IE, EL-Amrawi GA. Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. AJVS. 2023; 79(1): 142-149. doi:10.5455/ajvs.166702


Web Style

Ahmed DE, El-Bawab IE, EL-Amrawi GA. Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. https://www.alexjvs.com/?mno=166702 [Access: November 04, 2023]. doi:10.5455/ajvs.166702


AMA (American Medical Association) Style

Ahmed DE, El-Bawab IE, EL-Amrawi GA. Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. AJVS. 2023; 79(1): 142-149. doi:10.5455/ajvs.166702



Vancouver/ICMJE Style

Ahmed DE, El-Bawab IE, EL-Amrawi GA. Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. AJVS. (2023), [cited November 04, 2023]; 79(1): 142-149. doi:10.5455/ajvs.166702



Harvard Style

Ahmed, D. E., El-Bawab, . I. E. & EL-Amrawi, . G. A. (2023) Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. AJVS, 79 (1), 142-149. doi:10.5455/ajvs.166702



Turabian Style

Ahmed, Dina E., Iman E. El-Bawab, and Gamal A. EL-Amrawi. 2023. Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. Alexandria Journal of Veterinary Sciences, 79 (1), 142-149. doi:10.5455/ajvs.166702



Chicago Style

Ahmed, Dina E., Iman E. El-Bawab, and Gamal A. EL-Amrawi. "Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants." Alexandria Journal of Veterinary Sciences 79 (2023), 142-149. doi:10.5455/ajvs.166702



MLA (The Modern Language Association) Style

Ahmed, Dina E., Iman E. El-Bawab, and Gamal A. EL-Amrawi. "Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants." Alexandria Journal of Veterinary Sciences 79.1 (2023), 142-149. Print. doi:10.5455/ajvs.166702



APA (American Psychological Association) Style

Ahmed, D. E., El-Bawab, . I. E. & EL-Amrawi, . G. A. (2023) Enhancement of Cryo-preserved Brown Swiss Bull Semen Quality Using Different Antioxidants. Alexandria Journal of Veterinary Sciences, 79 (1), 142-149. doi:10.5455/ajvs.166702





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