E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Detection of Some Enteric Pathogens in Retailed Meat

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha.

Cited by (1)

Abstract
Contamination of meat at the retail level is very common. The presence of members of the family Enterobacteriaceae on the meat surface will render the meat unsafe to the consumer as they are encountered in causing food poisoning and also they reflect the hygienic standard of the butchers' shops. The current study was done to evaluate the microbiological status of retailed meat of cattle and buffaloes with special concern to Enteropathogenic Escherichia coli (EPEC) and Salmonella. A total of 100 random samples of meat of cattle and buffaloes (50 of each) were collected from different butchers' shops at Behera province. The samples were evaluated bacteriologically with a trial to isolate some enteric pathogens of public health significance. The obtained results revealed that the mean values of total Enterobacteriaceae count in examined meat samples of cattle and buffaloes were 1.4 X 1041.4 X 103 and 2.7 X 104 3.3 X 103 cfu/g, respectively. Also, the mean values of total Coliform count in cattle and buffaloes meat samples were 1.5 X 1041.4 X 103, 1.8 X104 1.8 X 103 cfu/g, respectively. In addition, isolation and identification of enteric bacteria revealed that the isolation of E. coli, Salmonella species, Enterobacter aerogenes. Enterobacter intermedium, Enterobacter gergoviae, Citrobacter amalonaticus, Citrobacter diversus, Citrobacter freundii, Serratia marcescens, Serratia ficaria, Serratia fonticola, Serratia liquefaciens. Serratia rubidaea, Edwardsiclla ictalori, Edwardsiella hoshinae, Providencia alcalifaciens, Providencia stuartii, Klebsiella pneumonia subspecies ozaenae, Proteus mirabilis. Concerning EPEC, it was found that it was detected in 32% and 40% of the examined meat samples of cattle and buffaloes, respectively. Furthermore, the serological identification of the obtained isolates of EPEC revealed the presence of the following serotypes O55: K59, O111:K58, O114:K96, O111:K69, O125:K70, O126:K71, O127:K63. On the other side, the obtained results revealed the isolation of Salmonella species at the percentage of 4 and 8 in the examined meat samples of cattle and buffaloes, respectively. Furthermore, the serological identification of the obtained isolates of Salmonella revealed the presence of S. Typhi, S. Enteritidis and S. Paratyphi. On basis of the obtained results it was observed that meat of buffaloes was more contaminated with all members of Enterobacteriaceae than meat of cattle so strict hygienic measures should be considered during slaughtering, transportation and handling of meat till reach safe for consumers.

Key words: Enterobacteriaceae, isolation, identification, meat, cattle, buffaloes


 
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REFERENCES
Ayhan, K., Özkan, G., Noveir, M.R. 2000. Determination of histidine decarboxylase activity produced by Enterobacteriaceae isolated from ground meat. J. Ciencia (10): 243 –250.
Castaras, M., Gulistani, A., Mossel,D. 1974. Surface contamination of refrigerated beef and horse carcasses. J. Recueil de Med. Vet. (150): 287-300.
Center for disease control and prevention (CDC). 2004. E. coli O157: H7.CDC. http://www. February cdc.gov/ncided/dbmd/ disease info/escherichiacoli-g. htm(accessed 12,2005).
Center for Disease Control, and Prevention (CDC), 2006. Division of Bacterial and Mycotic Disease. Disease information Escherichia coli O157: H7. MMWR Morb. Mortal. Wkly Rep., 54(2): 40.
Chambers, J. V., Berchbi, D. O., Hill, D. A. 1976. Microbiological survey of raw ground beef in chio. J. Milk Food Technol., (39): 530.
Cowan, S. T., Steel, K. 1974. Manual for identification of medical bacteria. Cambridge Univ. Press, London, New york, Malburne.
Doyle, M. P. 1991. Pathogenic E. coli. The Lancet, (336):1111-1115.
Edwards, P. R., Ewing, W. H. 1972. Identification of Enterobacteriaceae. 3rd Ed. Minneapolis, Burgess publishing Co. U.S.A
EI-Mossalami, M. K. 1988. Surface bacteria of slaughtered cattle in a modern abattoir M.V.Sc, thesis. Fac. Vet. Med. Cairo Univ.
Eley, A. R. 1992. Microbiological food poisoning published by champan hall 2-6 Boundary Raw. London.
Eribo, E., Jay, J. M. 1985. Incidence of acinetobacter spp. and other Gram negative oxidase bactera in fresh and spoiled ground beef. J. Appl. arid environ. Microbial. 49,(1): 256-257.
Feldman, D., Ganon, J., Hoffman, R., Simpson, J. 2003. The solution for data analysis and presentation graphics. 2nd Ed Abacus Lancripts, Inc., Berkeley, USA.
Fournaud, J., Graffino, G., Rosset, R., Jacque, R. 1978. Contamination microbienne des carcasses.a,l,abattoir. Industiies Ali-mentaires et Agricoles (95): 273-282.
Gork, F.P. 1976. Uber die ursachen von aualitatsmangein bei tiefgefroten fertiggerichten auf fleischbasic in der fluggast verp flegung. D. Ing. Diss.: Tu-Berlin.
Govindarajan, C. V. 1990. Maintenance of hygienic and sanitary conditions including personal hygiene in the meat factory. Technical paper in First National Seminar on Marketing of Meat Food Products in India, Aligarh, India.
Haeghebaert, S., Sulem, P., Deroudille, L., Vanneroy-Adenot, E., Bagnis, O., Bouvet, P., Grimont, F., Brisabois, A., Le Querrec, F., Hervy, C., Espié, E., de Valk, H., Vaillant V. 2003. Two outbreaks of Salmonella enteritidis phage type 8 linked to the consumption of Cantal cheese made with raw milk, France, 2001. Euro Surveill.; 8(7):151-156. [Pubmed]   
Hiko, A, Asrat, D., Zewde, G. 2008. Occurrence of Escherichia coli O157:H7 in retail raw meat products in Ethiopia. J. Infect. in Developing Countries. 2(5): 389-393.
International Commission on Microbiological specificatkn for foods (ICMSF) 1996. Microorganisms in foods 1.Their significance and methods of enumeration. 3rd Ed. Toronto, Univ. of Toronto Press
Jalali, M., Abedi, D., Pourbakhsh, S.A., Ghoukasin, K. 2008. Prevalence of Salmonella spp. in raw and cooked foods in Isfahan-Iran. J. Food Safety. 28 (3): 442-452. [DOI via Crossref]   
Jordan, D., Phillips, D., Sumner, J., Morris, S., Jenson, I. 2007. Relationships between the density of different indicator organisms on sheep and beef carcasses and in frozen beef and sheep meat. J. Appl. Microbiol. 102. (1): 57-64. [DOI via Crossref]    [Pubmed]   
Kauffman, F. 1974. Kauffman white scheme WHO-BD 172, h, Rev. 1. Actapath. Microbiol. Scand, 61:385.
Krieg, N.R., Holt, J.G. 1984. Bergey's Manual of systematic Bacteriology. Vol. 1, Williams, Baltimore, London.
Marrito, N.G., Gravani, R. B 2006. Principles of food sanitation 5th edition, 2006. Springer Science Business Media.Inc.
Mercuri, A.J., Cosx, N.A. 1979. Coliforms and Enterobacteriaceae isolated from selected food. J. Food Prot., (42): 712.
Mira, E.K. 1989. Hygienic status of beef produced in new Cairo abattoirs. M.V.Sc. Thesis. Fac. Vet. Med. Cairo Univ.
Miranda, J., Guarddon M., Vázquez B.I., Fente C.A., BarrosVelázquez J., Cepeda, Mousa, M., Bkhiet, A. A., Eid, M. 2000. Bacteriological aspects of precooked meat in Damanhour. Egypt. J. Agricult. Res. (78): 261-272.
Much, P., Pichler, J., Allerberger, F. 2007. Food borne infectious outbreaks, Austria 2005. J. Wien Klin Wochenschr.; 119(5-6):150-157. [DOI via Crossref]    [Pubmed]   
Paterson. D. L. 2006. Resistance in Gram-negative bacteria (Enterobacteriaceae). Am. J. Med.119: 520–528. [DOI via Crossref]    [Pubmed]   
Pyatkin, K. 1967. Microbiology. 1st Ed. Mir Publishers Moscow, P. 356.
Shewan, J. M. 1971. A progress report. J. Appl. Bacteriol., (34): 299-315. [DOI via Crossref]    [Pubmed]   

This Article Cited By the following articles

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Afr. J. Biotechnol. 2018; 17(2): 29.

1
 
How to Cite this Article
Pubmed Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. Detection of Some Enteric Pathogens in Retailed Meat. AJVS. 2015; 44(1): 67-73. doi:10.5455/ajvs.171536


Web Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. Detection of Some Enteric Pathogens in Retailed Meat. http://www.alexjvs.com/?mno=171536 [Access: August 17, 2021]. doi:10.5455/ajvs.171536


AMA (American Medical Association) Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. Detection of Some Enteric Pathogens in Retailed Meat. AJVS. 2015; 44(1): 67-73. doi:10.5455/ajvs.171536



Vancouver/ICMJE Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. Detection of Some Enteric Pathogens in Retailed Meat. AJVS. (2015), [cited August 17, 2021]; 44(1): 67-73. doi:10.5455/ajvs.171536



Harvard Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha (2015) Detection of Some Enteric Pathogens in Retailed Meat. AJVS, 44 (1), 67-73. doi:10.5455/ajvs.171536



Turabian Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. 2015. Detection of Some Enteric Pathogens in Retailed Meat. Alexandria Journal of Veterinary Sciences, 44 (1), 67-73. doi:10.5455/ajvs.171536



Chicago Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. "Detection of Some Enteric Pathogens in Retailed Meat." Alexandria Journal of Veterinary Sciences 44 (2015), 67-73. doi:10.5455/ajvs.171536



MLA (The Modern Language Association) Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha. "Detection of Some Enteric Pathogens in Retailed Meat." Alexandria Journal of Veterinary Sciences 44.1 (2015), 67-73. Print. doi:10.5455/ajvs.171536



APA (American Psychological Association) Style

Mohammad A. Nossair, Kamal Khaled, Nahla A. El Shabasy and Ibrahim A. Samaha (2015) Detection of Some Enteric Pathogens in Retailed Meat. Alexandria Journal of Veterinary Sciences, 44 (1), 67-73. doi:10.5455/ajvs.171536





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