E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province

Noha A. Beder, Adel A. Fayed.

Abstract
A total number of 250 calves were subjected to thorough clinical examination and rectal swabbing for bacterial isolation. These calves were divided into two groups; the first one was123 calves which appeared healthy with shiny eyes and bloomy skin. The second group was 127 calves with diarrhea, different degrees of dehydration and most of them were separated from their dams before weaning age and did not take the colostrum. The most common bacterial isolates were Escherichia coli (E.coli) (100 isolates, 39.7%). The quinolone resistance phenotypes of E.coli isolates were recorded in 50 calves (42 diarrheic and 8 healthy calves); nalidixic acid (NAL 26%), norfloxacin (NOR 29%), ciprofloxacin (CIP 23 %) and enrofloxacin (ENRO 33%). PCR amplification reaction of twenty resistant E.coli isolates detected the plasmid- mediated quinolone resistance genes; qnrA, in four isolates (diarrheic calves) and aac(6’)Ib-cr in sex isolates (four diarrheic and two of healthy calves). We concluded that several mechanisms of quinolones resistance are present in enteric E.coli from diarrheic as well as healthy calves. These resistances increase the public health hazard of these bacteria.

Key words: Calf, Diarrhea, E.coli, Quinolone resistance


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Noha A. Beder
Articles by Adel A. Fayed
on Google
on Google Scholar


REFERENCES
Berge, A.C., Atwill, E.R., Sischo, W.M. 2009. Animal and farm influences on the dynamics of antibiotic resistance in fecal E. coli in young dairy calves. Prev. Vet. Med. 69: 25-38. [DOI via Crossref]    [Pubmed]   
Cavaco, L.M., Aarestrup, F.M. 2009. Evaluation of quinolones for use in detection of determinants of acquired quinolone resistance, including the new transmissible resistance mechanisms qnrA, qnrB, qnrS, and aac(6')Ib-cr, in Escherichia coli and Salmonella enterica and determinations of wild-type distributions. J. Clin. Microbiol. 47: 2751-2758. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Clinical and Laboratory Standards Institute. 2006. Performance Standards for Disk Susceptibility Tests; Approved Standard M2-A8, 8th edn.Wayne, PA, USA: CLSI.
Collins, C., Lyne, P., Grange, J. 1991. Microbiological methods. 6th Ed., Microbiology Laboratory Manual, British Library, Butterworth Inc., London, UK.
Di Labio, E., Regula, G., Steiner, A., Miserez, R., Thomann, A. and Ledergerber, U. 2007. Antimicrobial resistance in bacteria from Swiss veal calves at slaughter. Zoon. Public Health 54:344-352. [DOI via Crossref]    [Pubmed]   
Donaldson, S.C., Straley, B.A., Hegde, N.V., Sawant, A.A., Debroy, C., Jayarao, B.M. 2006. Molecular epidemiology of ceftiofur-resistant E. coli isolates from dairy calves. Appl. Environ. Microbiol. 72: 3940-3948. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Eaves, D.J., Randall, L., Gray, D.T., Buckley, A., Woodward, M.J., White, A.P., Piddock, L.J. 2004. Prevalence of mutations within the quinolone resistance-determining region of gyrA, gyrB, parC, and parE and association with antibiotic resistance in quinolone-resistant Salmonella enterica. Antimicrob. Agents Chemother. 48: 4012–4015. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Edwards, P.R., Ewing, W.H. 1972. Identification of Enterobacteriaceae. 3rd Ed. Minneapolis. Burgess Publishing Co. U.S.A
EMA . 2012. Sales of Veterinary Antimicrobial Agents in 19EU/EEA Countriesin (Second ESVAC report). European Medicines Agency, London, United Kingdom enterotoxigenic, enteropathogenic, enteroinvasive, enterohaemorrhagic and enteroadherent.
Faber, S.N., Faber, N.E., Mc Cauley, T.C. 2005. Case Study: Effects of colostrum ingestion on lactational performance. Prof. Anim. Scientist 21: 420-425.
Fourichon, C., Seegers, H., Beuadeau, F., Verfaille, L., Bareille, N. 2001. Health control costs in dairy farming systems in Western Europe. Livest. Prod. Sci. 68: 141–156. [DOI via Crossref]   
Gibson, J. S., Cobbold, R.N., Heisig, P., Sidjabat, H. E. and Kyaw-Tanner, M.T., Trott, D. J. 2010. Identification of Qnr and AAC(6')-1b-cr plasmid-mediated fluoroquinolone resistance determinants in multidrug-resistant Enterobacter spp. isolated from extraintestinal infections in companion animals Vet. Microbiol. 143: 329-336. [DOI via Crossref]    [Pubmed]   
Hadorn, U., Hammon, H., Bruckmaier, R.M. 1997. Delaying colostrum intake by one day has important effects on metabolic traits and on gastrointestinal and metabolic hormones in neonatal calves. J. Nutr. 127: 2011-2023. [Pubmed]   
Hassan, W.M., Hashim, A. and Domany, R.A.A. 2012. Plasmid mediated quinolone resistance determinants qnr, aac(6β€²)-Ib-cr Indian Journal of Med. Microbiol. 30: 442-447. [DOI via Crossref]    [Pubmed]   
Holland, R.E. 1990. Some infectious causes of diarrhea in young farm animals. Clin. Microbiol. Rev. 3:345- 375. [Pubmed]    [PMC Free Fulltext]   
Hordijk, J., Bosman, A.B., Van Essen-Zandbergen, A. 2011. qnrB19 Gene Bracketed by IS26 on a 40-Kilobase IncR Plasmid from an Escherichia coli Isolate from a Veal Calf. Antimicrob. Agents Chemothera. 55: 453-454. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Jacoby, G.A., Walsh, K.E., Mills, D.M., Walker, V.J., Oh, H., Robicsek, A., Hooper, D.C. 2008. qnrB, another plasmid-mediated gene for quino-lone resistance. Antimicrob. Agents Chemother. 50:1178–1182. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Joost Hordijk1, Kees Veldman, Cindy Dierikx, Alieda van Essen-Zandbergen, Jaap A. Wagenaar, Dik Mevius 2012. Prevalence and characteristics of quinolone resistance in E. coli in veal calves Vet. Microbiol. 156:136-142.
Jones-Dias, Manageiro, V., Francisco, A.P., Martins, A.P., Domingues, G., Louro, D., Ferreira, E., CanicΒΈ M. 2013. Assessing the molecular basis of transferable quinolone resistance in Escherichia coli and Salmonella spp. From food-producing animals and food products Vet. Microbiol. 167:523-531. [DOI via Crossref]    [Pubmed]   
Khan, A., Zaman, T. 2007. Effects of rehydration solution on hematological and biochemical parameters in induced buffalo neonatal calf diarrhea. Ital. J. Anim. Sci. 6: 957-960
Kim, M.H., Lee, H.J., Park, K.S., Suh, J.T. 2010. Molecular characteristics of extended spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae and the prevalence of qnr in extended spectrum beta-lactamase isolates in a tertiary care hospital in Korea. Yonsei Med. J. 51: 768-774. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Kovacs, N. 1978. A simplified method for detection indol formation by bacteria. Immunitats forsch. 56: 311 - 313.
Levine, C., Hiasa, H., Marians, K.J. 1998. DNA gyrase and topoisomerase IV. 1998. Biochemical activities, physiological roles during chromosome replication, and drug sensitivities. Biochim. Biophys. Acta. 1400:29–43. [DOI via Crossref]   
Ljutov, V. 1963. Technique of voges - Proskauar test. Actapathol. Microbiol. Scand. 58: 325- 335. [DOI via Crossref]    [Pubmed]   
MacFaddin, J.F. 1976. Biochemical tests for identification of medical bacteria. Wavery Press, INC., Baltimore, Md. 21202 USA
Martinez, J.L., Baquero, F. 2002. Interactions among strategies associated with bacterial infection: pathogenicity, epidemicity, and antibiotic resistance. Clin Microbiol. Rev. 15: 647-679. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Metasebia, A., Tesfaye, S., Genene, T., Belay T. 2013. Identification and Biotyping of Esherichia coli from Diarrheic Lambs in/and Around Debre Birhan Town, Ethiobia. J. Environ. Anal. Toxicol. 3 :1-5.
Odea, T., Saitob, R., Kumitaa, W., Satoa, K., Okugawab, S., Moriyab, K., Koikeb, K., Okamura, N. 2009. Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan. Int. J. Antimicrob. Agents 34: 347-350. [DOI via Crossref]    [Pubmed]   
Pallecchi, L., Bartoloni, A., Fiorelli, C., Manetella, A., Tiziana Di Maggio., Gamboa, H., Gotuzzo, E. and Gian Maria Rossolini 2007. Rapid Dissemination and Diversity of CTX-M Extended-Spectrum B Lactamase Genes in Commensal E. coli isolated from Healthy Children in Latin America. Antimicrobial. Agents. Chemother. 51: 2720-2725.
Park, C.H., Robicsek, A., Jacoby, G.A., Sahm, D. Hooper, D.C. 2006. Prevalence in the United States of aac(6')-Ib-cr encoding a ciprofloxacinmodifying enzyme. Antimicrob. Agents Chemother. 50: 3953–3955. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Poirel, L., Cattoir, V., Nordmann, P. 2012. Plasmid-mediated quinolone resistance: interactions between human, animal, and environmental ecologies. Front. Microbiol. 3: 1-7. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D., 2007. Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats, tenth ed. Elsevier Health Sciences, Philadelphia, PA, USA.
Rainard, P. 2005. Tackling mastitis in dairy cows. Nat Biotechnol 23: 430–432. [DOI via Crossref]    [Pubmed]   
Robicsek, A., Strahilevitz, J., Jacoby, G.A., Macielag, M., Abbanat, D., Park,C.H., Bush, K., Hooper, D.C. 2006. Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase Nat. Med. 12: 83–88. [DOI via Crossref]    [Pubmed]   
Simmon, J.S. 1926. A culture medium for differentiating organisms of the typhoid - aerogenes groups and the isolation of certain fungi. J. Infect. Dis. 39: 209- 214. [DOI via Crossref]   
Simon, le hello, Anne Brisabios, MarieAcco-Dermatin, Adeline Josse, Muriel Mrault, Sylvie Francart, Nathalie Jourdan Da Silva., Francois Xavier Weill .2012. Food borne outbreak and Non motile Salmonella enterica Variant, France. Emerg. Infect. Dis. 18: 132-134.
Stephenson, S., Brown, P.D., Holness, A., Wilks, M. 2010. The Emergence of qnr-mediated quinolone resistance among Enterobacteriaceae in Jamaica. West. Indian Med. J. 59: 241-244. [Pubmed]   
Strahilevitz, J., Jacoby, G.A., Hooper, D.C., Robicsek, A. 2009. Plasmid-mediated quinolone resistance: a multifaceted threat Clin. Microbiol. Rev., 22: 664–689. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Tamang, M.D., Nam, H.M., Chae, M.H., Kim, S.R., Gurung, M., Jang, G.C., Jung, S.C., Lim, S.K. 2012. Prevalence of plasmid-mediated quinolone resistance determinants among Escherichia coli isolated from food animals in Korea. Food borne Pathog. Dis. 9:1057-1063. [DOI via Crossref]    [Pubmed]   
Tran, J.H., Jacoby, G.A. 2002. Mechanism of plasmid-mediated quinolone resistance. Proc. Natl. Acad. Sci. U.S.A. 99: 5638–5642. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Travis, R.M., Gyles, C.L., Reid-Smith, R., Poppe, C., McEwen, SA., Friendship, R., Janecko, N., Boerlin, P. 2006. Chloramphenicol and kanamycin resistance among porcine Escherichia coli in Ontario. The Journal of Antimicrobial Chemotherapy 58:173-177. [DOI via Crossref]    [Pubmed]   
Veldman, K., Cavaco, L.M., Mevius, D. 2011. International collaborative study on the occurrence of plasmid-mediated quinolone resistance in Salmonella enterica and Escherichia coli isolated from animals, humans, food and the environment in 13 European countries. J. Antimicrob. Chemoth. 66: 1278-1286. [DOI via Crossref]    [Pubmed]   
Walk, S.T., Mladonicky, J.M., Middleton, J.A., Heidt, A.J., Cunningham, J.R., Bartlett, P., Sato, K., Whittam, T.S. 2007. Influence of antibiotic selection on genetic composition of Escherichia coli populations from conventional and organic dairy farms. Appl. Environ. Microbiol. 73:5982-5989. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Wang, M., Guo, Q., Xu, X., Wang, X., Ye, X., Wu, S., Hooper, D.C. 2009. New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis. Antimicrob. Agents Chemother. 53: 1892– 1897. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Yaqub Ahmed Geidam, Zunita Zakaria, Saleha Abdul Aziz, Siti Khairani Bejo, Jalila Abu, Sharina Omar 2012. High Prevalence of Multi-drug Resistant Bacteria in Selected Poultry Farms in Selangor, Malaysia. Asian Journal of Animal and Veterinary Advances, 7: 891-897. [DOI via Crossref]   

How to Cite this Article
Pubmed Style

Noha A. Beder, Adel A. Fayed. Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. AJVS. 2015; 47(1): 1-6. doi:10.5455/ajvs.199067


Web Style

Noha A. Beder, Adel A. Fayed. Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. http://www.alexjvs.com/?mno=199067 [Access: August 16, 2021]. doi:10.5455/ajvs.199067


AMA (American Medical Association) Style

Noha A. Beder, Adel A. Fayed. Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. AJVS. 2015; 47(1): 1-6. doi:10.5455/ajvs.199067



Vancouver/ICMJE Style

Noha A. Beder, Adel A. Fayed. Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. AJVS. (2015), [cited August 16, 2021]; 47(1): 1-6. doi:10.5455/ajvs.199067



Harvard Style

Noha A. Beder, Adel A. Fayed (2015) Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. AJVS, 47 (1), 1-6. doi:10.5455/ajvs.199067



Turabian Style

Noha A. Beder, Adel A. Fayed. 2015. Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. Alexandria Journal of Veterinary Sciences, 47 (1), 1-6. doi:10.5455/ajvs.199067



Chicago Style

Noha A. Beder, Adel A. Fayed. "Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province." Alexandria Journal of Veterinary Sciences 47 (2015), 1-6. doi:10.5455/ajvs.199067



MLA (The Modern Language Association) Style

Noha A. Beder, Adel A. Fayed. "Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province." Alexandria Journal of Veterinary Sciences 47.1 (2015), 1-6. Print. doi:10.5455/ajvs.199067



APA (American Psychological Association) Style

Noha A. Beder, Adel A. Fayed (2015) Prevalence and Characterization of Quinolones Resistant E.Coli From Calves in Kafr-Elsheikh Province. Alexandria Journal of Veterinary Sciences, 47 (1), 1-6. doi:10.5455/ajvs.199067





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]