E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied.

Abstract
Shiga toxin-producing Escherichia coli (STEC) are foodborn pathogens cause mild to serious diseases, leading to people death. This study reports the prevalence and characterization of STEC O157 and non-O157 in beef retail markets (commercial ground beef and instruments samples: meat tables, knives, meat mincing machines) obtained from 25 retail markets in Kafer El-sheikh governorate. The STEC isolates were serotyped and virulence genes as stx1 (Shiga toxin 1), stx2 (Shiga toxin 2) and eae (intimin) were determined. STEC O157 were identified in 11 (14.6 %) of raw beef samples and 8 (10.6%) in instruments samples, while STEC non-O157 (O128, O78, O111, O26 and O119) were present in 6(8%) in raw beef samples and 9 (12%) in instruments samples. Among strains isolated , the prevalent genotype for O157 were stx1,stx2and eae while for STEC non-O157 were stx1 , stx2 and eae in O111, stx1 and stx2 in O26 , stx1 in O128 and O119 , stx2 in O78 . In conclusion, cross-contamination between meat and the environment could be suspected.

Key words: shiga toxin, STEC, raw ground beef, retail markets, instruments samples, molecular.


 
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REFERENCES
Ahmed, A.M., Shimamoto, T. 2015. Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products. Int. J. Food. Microbiol. 193:68-73. [DOI via Crossref]    [Pubmed]   
Ali, M.M., Ahmed, S.F., Klena, J.D., Mohamed, Z.K., Moussa, T.A., Ghenghesh, K. S. 2014. Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility. J. Infect. Dev. Ctries. 14 (5): 589-596. [DOI via Crossref]   
Arthur, T. M., James, L. B., Norasak, K. 2014. Characterization of Escherichia coli O157:H7 Strains from Contaminated Raw Beef Trim during "High Event Periods" Appl Environ. Microbiol. 80 (2): 506–514. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Barkocy-Gallagher, G.A., Arthur, T.M., Siragusa, G.R., Keen, J.E., Elder, R.O., Laegreid, W.W., Koohmaraie, M. 2001. Genotypic analyses of Escherichia coli O157: H7 and non-O157 isolates recovered from beef cattle and carcasses at processing plants in the Midwestern states of the United States. Appl. Environ. Microbiol. 67: 3810–3818 [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Brooks, J.T., Sowers, E.G., Wells, J.G. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983–2002. J. Infec.t Dis. 192:1422–1429. [DOI via Crossref]    [Pubmed]   
De Boer, E. and Heuelink, A.E. 2000. Method for detection and isolation of Shiga Toxin-Producing Escherichia coli. Symp. Ser. Soc. Appl. Microbiol. (29):1335-1435.
DebRoy, C., Roberts, E., Valadez, A.M., Dudley, E.G., Cutter, C.N. 2011. Detection of Shiga toxin-producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster. Foodbone Pathog. Dis. 8: 651–652. [DOI via Crossref]    [Pubmed]   
Dhanashree, B. and Mallya, S. 2008. Detection of shiga-toxigenic Escherichia coli (STEC) in diarrhoeagenic stool and meat samples in Mangalore, India. Indian J. Med. Res., 128: 271-277. [Pubmed]   
Edwards, J. R., Fung, D. Y. C. 2006. Prevention and decontamination of Escherichia coli O157:H7 on raw beef carcasses in commercial beef abattoirs. J. Rapid Methods Autom. Microbiol. 14: 1–95. [DOI via Crossref]   
Erickson, M. C. and Doyle, M. P. 2007. Food as a vehicle for transmission of Shiga toxin-producing Escherichia coli. J. Food Prot. 70:2426–2449. [Pubmed]   
Ewing, W.H., Edwards, P. R. 1986. Edwards and Ewing, s Identification of Enterobacteriaceae. New York, N Y: El Seiver.
Fei Wang, L., Jiang I.N., Beilei, G.E. 2012. Loop-Mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli in Ground Beef and Human Stools. J Clin. Microbiol. 50(1): 91–97. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Grant, M.A., Hedberg, C., Johnson, R. 2011. Executive summary. The significance of non-O157 Shiga toxin-producing Escherichia coli in food. Food Prot. Trends. 33–42.
Gyles, C.L. 2007. Shiga toxin-producing Escherichia coli: An overview. J. Anim. Sci. 66: E45–E62. [DOI via Crossref]    [Pubmed]   
Hsu, H., Sheen, S., Sites, J., Cassidy, J., Scullen, B. Sommers, C . 2015. Effect of High Pressure Processing on the survival of Shiga Toxin-Producing Escherichia coli (Big Six vs. O157:H7) in ground beef. Food Microbiol. 48:1-7. [DOI via Crossref]    [Pubmed]   
Hussein, H.S., Bollinger, L.M. 2005. Prevalence of Shiga toxin-producing Escherichia coli in beef. Meat Sci. 71:676–689. [DOI via Crossref]    [Pubmed]   
Jeshveen, S., Chai, L., Pui, C., Son, R. 2013. Optimization of multiplex PCR conditions for rapid detection of Escherichia coli O157:H7 virulence 19(2): 461-466.genes. Inter. Food Res. J. 19(2): 461-466.
Johnson, R. P., Clarke, R.C. and Wilson, J. B. 1996. Growing concerns and recent outbreaks involving non-O157:H7 verotoxigenic Escherichia coli . J Food Prot 59:1112–1122.
Joseph, M., B. and Mohammad K., 2011. Prevalence and Characterization of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Commercial Ground Beef in the United States. Appl. Environ. Microbiol. 77(6): 2103–2112. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Karmali, M. A., Gannon, V., Sargeant J. M. 2010. Verocytotoxin- producing Escherichia coli (VTEC). Vet. Microbiol. 140: 360–370. [DOI via Crossref]    [Pubmed]   
Karmali, M.A., Mascarenhas, M. and Shen, S. 2003.Association of genomic O Isl and 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J. Clin. Microbiol. 41 (11): 4930–4940. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Kok, T., Worswich, D., Gowans, E. 1996. Some serological techniques for microbial and viral infections. In Practical Medical Microbiology (Collee, J.; Fraser, A.; Marmion, B. and Simmons, A., eds.), 14th ed., Edinburgh, Churchill Livingstone, UK.
Kudakwashe, M., Huu A. D., Edward W. M., Catherine N. C., Elisabeth L. R., Chitrita D. 2013. Incidence of Shiga toxin–producing Escherichia coli strains in beef, pork, chicken, deer, boar, bison, and rabbit retail meat. J. Vet. Diagnost. Investigat. 25 (2) 254-258. [DOI via Crossref]    [Pubmed]   
Madic, J., Vingadassalon, N., and de Garam, C.P. 2011. Detection of Shiga toxin-producing Escherichia coli serotypes O26:H11, O103:H2, O111:H8, O145:H28, and O157:H7 in raw-milk cheeses by using multiplex real-time PCR. Appl. Environ. Microbiol. 77(6): 2035–2041. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Mostafa, M. A., Salwa, F. A., John, D. K., Zienat, K. M., Tarek, A. M., Khalifa, S. G. 2014. Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility. J. Infect. Dev. Ctries. 14(5):589-596.
Paton, A.W., Paton, J.C. 2002. Direct detection and characterization of shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol.40 (1): 271–274. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Patricia, L., Laura, B., Kinue, I., María, V. R., Adriana B. 2014.Characterization of Shiga Toxin-Producing Escherichia coli Isolated from Ground Beef Collected in Different Socioeconomic Strata Markets in Buenos Aires, Argentina. Biomed Res. Int. 1-9.
Sallam, K.I., Mohammed, M.A., Ahdy, A.M. and Tamura, T. 2013. Prevalence, genetic characterization and virulence genes of Sorbitol-fermenting Escherichia coli O157:H7 isolated from retail beef. Int J. Food Microbiol. 165 (3): 295-301. [DOI via Crossref]    [Pubmed]   
Scallan, E., Hoekstra, R.M., Angulo, F.J. 2011. Foodborne illness acquired in the United States—major pathogens. Emerg. Infect. Dis. 17: 7–15. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Shah, D., Shringi, S., Besser, T., Call, D. 2009. Molecular detection of foodborne pathogens, Boca Raton: CRC Press, In Liu, D. (Ed). Taylor & Francis group, Florida, USA, Pp. 369-389. [Pubmed]   
Sipos, R., Anna, J., Marton, P., Sara, R., Karoly, M. and Marcell, N. 2007. Effect of primer mismatch, annealing temperature and PCR cycle number on16S rRNA gene-targetting bacterial community analysis. FEMS Microbial. Ecol. 60: 341-350 [DOI via Crossref]    [Pubmed]   
Sommers, C., Rajkowski, K.T., Scullen, O.J., Cassidy, J., Fratamico, P., Sheen, S. 2015 . Inactivation of Shiga Toxin-Producing Escherichia coli in lean ground beef by gamma irradiation. Food Microbiol. 49:231-234. [DOI via Crossref]    [Pubmed]   
Terrance, M. A., James, L. B., Norasak K.M. 2014. Characterization of Escherichia coli O157:H7 Strains from Contaminated Raw Beef Trim during "High Event Periods" Appl. Environ. Microbiol. 80(2): 506–514. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Valadez, A.M., Debroy, C., Dudley, E., Cutter, C.N. 2011. Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef. J. Food. Prot. 74: 228–239. [DOI via Crossref]    [Pubmed]   
Xiaodong, X., Jianghong, M., Patrick, F., McDermott, S., Karen, B., Thu-Thuy, T., Jason, A., Jie, Z., Shaohua, Z. 2010 . Presence and Characterization of Shiga Toxin-Producing Escherichia coli and Other Potentially Diarrheagenic E. coli Strains in Retail Meats. Appl. Environ. Microbiol. 76(6): 1709–1717. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

How to Cite this Article
Pubmed Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. 2016; 48(1): 50-56. doi:10.5455/ajvs.205072


Web Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. http://www.alexjvs.com/?mno=205072 [Access: August 17, 2021]. doi:10.5455/ajvs.205072


AMA (American Medical Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. 2016; 48(1): 50-56. doi:10.5455/ajvs.205072



Vancouver/ICMJE Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. (2016), [cited August 17, 2021]; 48(1): 50-56. doi:10.5455/ajvs.205072



Harvard Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied (2016) Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS, 48 (1), 50-56. doi:10.5455/ajvs.205072



Turabian Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. 2016. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. Alexandria Journal of Veterinary Sciences, 48 (1), 50-56. doi:10.5455/ajvs.205072



Chicago Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. "Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets." Alexandria Journal of Veterinary Sciences 48 (2016), 50-56. doi:10.5455/ajvs.205072



MLA (The Modern Language Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. "Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets." Alexandria Journal of Veterinary Sciences 48.1 (2016), 50-56. Print. doi:10.5455/ajvs.205072



APA (American Psychological Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied (2016) Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. Alexandria Journal of Veterinary Sciences, 48 (1), 50-56. doi:10.5455/ajvs.205072





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