E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied.

Abstract
Shiga toxin-producing Escherichia coli (STEC) are foodborn pathogens cause mild to serious diseases, leading to people death. This study reports the prevalence and characterization of STEC O157 and non-O157 in beef retail markets (commercial ground beef and instruments samples: meat tables, knives, meat mincing machines) obtained from 25 retail markets in Kafer El-sheikh governorate. The STEC isolates were serotyped and virulence genes as stx1 (Shiga toxin 1), stx2 (Shiga toxin 2) and eae (intimin) were determined. STEC O157 were identified in 11 (14.6 %) of raw beef samples and 8 (10.6%) in instruments samples, while STEC non-O157 (O128, O78, O111, O26 and O119) were present in 6(8%) in raw beef samples and 9 (12%) in instruments samples. Among strains isolated , the prevalent genotype for O157 were stx1,stx2and eae while for STEC non-O157 were stx1 , stx2 and eae in O111, stx1 and stx2 in O26 , stx1 in O128 and O119 , stx2 in O78 . In conclusion, cross-contamination between meat and the environment could be suspected.

Key words: shiga toxin, STEC, raw ground beef, retail markets, instruments samples, molecular.


 
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How to Cite this Article
Pubmed Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. 2016; 48(1): 50-56. doi:10.5455/ajvs.205072


Web Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. https://www.alexjvs.com/?mno=205072 [Access: December 03, 2023]. doi:10.5455/ajvs.205072


AMA (American Medical Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. 2016; 48(1): 50-56. doi:10.5455/ajvs.205072



Vancouver/ICMJE Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS. (2016), [cited December 03, 2023]; 48(1): 50-56. doi:10.5455/ajvs.205072



Harvard Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied (2016) Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. AJVS, 48 (1), 50-56. doi:10.5455/ajvs.205072



Turabian Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. 2016. Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. Alexandria Journal of Veterinary Sciences, 48 (1), 50-56. doi:10.5455/ajvs.205072



Chicago Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. "Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets." Alexandria Journal of Veterinary Sciences 48 (2016), 50-56. doi:10.5455/ajvs.205072



MLA (The Modern Language Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied. "Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets." Alexandria Journal of Veterinary Sciences 48.1 (2016), 50-56. Print. doi:10.5455/ajvs.205072



APA (American Psychological Association) Style

Adel M. El-Gamal, Abdou M.S., Nehad I.E. Salem, Nahla A.A. Ebied (2016) Isolation and Molecular Characterization of Shiga Toxin-Producing Escherichia Coli in Beef Retail Markets. Alexandria Journal of Veterinary Sciences, 48 (1), 50-56. doi:10.5455/ajvs.205072





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