E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt

Alsagher O. Ali.

Cited by (2)

Abstract
Abstract
Staphylococcal protein A (spa) is an important virulence factor of S. aureus and it is considered one of the significant genes that used in the molecular epidemiological investigations. 240 milk samples were collected from animals with no signs of clinical mastitis; dairy cattle (140) and she-camels (100). This study was concerned with the analysis of the molecular characterizations of SPA genes extracted from the whole bacterial chromosome of 13 S. aureus isolates from cattle (9) and camels (4) raised in Egypt. According to the mecA gene, the S. aureus strains were divided into 7 methicillin resistant S. aureus (MRSA) and 6 methicillin susceptible S. aureus (MSSA). Analysis of the different SPA sequences revealed the presence of 8 SPA types; the most common SPA type was t359 that found in 5 isolates of S. aureus (all of them MRSA). By conducting the multiple sequence alignment, it revealed the presence of 7 conserved regions and 2 highly variable regions. The phylogenetic analysis of the SPA sequences of S. aureus local isolates and those were retrieved from the GENEBANK revealed 2 main clusters; the first one contained all local sequences in addition to EU695225. The rest of retrieved sequences were gathered in the second cluster.

Key words: Keywords: Staphylococcal protein A (SPA), molecular epidemiology, MRSA & MSSA


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Alsagher O. Ali
on Google
on Google Scholar


REFERENCES
Aires-de-Sousa, M., Parente, C. E., Vieira-da-Motta,O., Bonna, I. C., Silva, D. A., de Lencastre, H. 2007. Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro State, Brazil. Appl. Environ. Microbiol. 73: 3845-3849.
Cui, S., Li, J., Hu, C., Jin, S., Li, F., et al. 2009. Isolation and characterization of methicillin-resistant Staphylococcus aureus from swine and workers in China. J. Antimicrob. Chemotherap. 64: 680-683.
Fessler, A., et al. 2010. Characterization of methicillin-resistant Staphylococcus aureus ST398 from cases of bovine mastitis. J. Antimicrob. Chemother.65:619-625. [DOI via Crossref]    [Pubmed]   
Hallin, M., A. Deplano, O. Denis, R. De Mendonca, R. de Ryck, M. J. Struelens. 2007. Validation of pulsed-field gel electrophoresis and spa typing for long term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections. J. Clin. Microbiol. 45:127-133. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Hata, E., Ken, K., Hideki, K., Ikuo, U., Kiyoshi, T., Masashi, E. 2010. Genetic Variation among Staphylococcus aureus Strains from Bovine Milk and Their Relevance to Methicillin-Resistant Isolates from Humans. J. Clin. Microbiol. p. 2130-2139.
Huber, H., Koller, S., Giezendanner, N., Stephan, R., Zweifel, C. 2009. Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland. Euro Surveill. 15:1- 4.
Ko¨ck, R., Becker, K., Cookson, B., van Gemert-Pijnen, J. E., Harbarth., S., et al. 2010. Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Euro surveillance: bulletin europe´en sur les maladies transmissible = European communicable disease bulletin 15: 19688.
Kumar, S., Gadagkar, S. R. 2001. Disparity Index: A simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics 158: 1321-1327.
Larsen, J., Skov, R. and Larsen, A. R. 2013. Epidemiology of methicillin-resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans. Clin. Microbiol. Infect. 19: E16-E22.
Malachowa, N., Sabat, A., Gniadkowski, M. et al. 2005. Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing for clonal characterization of Staphylococcus aureus isolates. J Clin. Microbiol. 43: 3095-3100.
Monecke, S., Ehricht, R., Slickers, P., Wernery, R., Johnson, B., Jose, S., Wernery, U. 2011. Microarray-based genotyping of Staphylococcus aureus isolates from camels. Vet. Microbiol. 150 (3-4):309-314.
Nakagawa, S., Taneike, I., Mimura, D. 2005. Gene sequences and specific detection for Panton-Valentine leukocidin. Biochem Biophys Res. Commun. 328: 995-1002.
Petersen, A., Stegger, M., Heltberg, O., Christensen, J., Zeuthen, A., Knudsen, L. K., Urth, T., Sorum, M., Schouls, L. 2013. Epidemiology of methicillin-resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans. Clin. Microbiol. Infect. 19: E16-E22.
Paterson, G.K., Ewan, M. H., Mark, A. H. 2014. The emergence of mec C methicillin-resistant Staphylococcus aureus. Trends Microbiol. 22 (1): 42-47. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Spohr, M, Rau, J., Friedrich, A., Klittich, G. Fetsch, A., Guerra, B., Hammerl, J. A., Tenhagen, B.-A. 2011. Methicillin-resistant Staphylococcus aureus (MRSA) in three dairy herds in southwest Germany. Zoon. Public Health 58: 252-261.
Ruppitsch, W., Indra, A., Stoger, A., Mayer, B., Stadlbauer, S., Wewalka, G., F. Allerberger. 2006. Classifying spa types in complexes improves interpretation of typing results for methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 44:2442-2448. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Saei, H. D., Ahmadi, M. 2011. “Discrimination of Staphylococcus aureus isolates on the basis of gene coding protein Ausing PCRrestriction enzyme analysis,” Comparative Clin. Pathol. 21(5): 645-652.
Shakeri, F., Shojai, A., Golalipour, M., Alang,S. R., Vaez, H., E. A. Ghaemi, E. A. 2010. “Spa diversity among MRSA and MSSA strains of Staphylococcus aureus in north of Iran,” Int. J. Microbiol. 5 pages 351397.
Strommenger, B., Braulke, C., Heuck, D., Schmidt, C., Pasemann, B., Nu¨bel, U., and Witte, W. 2008. spa Typing of Staphylococcus aureus as a Frontline Tool in Epidemiological Typing. J. Clin. Microbiol. 46 (2). 574-581.
Strommenger, B., Kettlitz, C., Weniger, T. Harmsen, D., Friedrich, A. W., Witte, W. 2006. Assignment of Staphylococcus isolates to groups by spa typing, SmaI macrorestriction analysis, and multilocus sequence typing. J. Clin. Microbiol. 44:2533-2540. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
Tamura, K., Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evolut. 10:512-526.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution30: 2725-2729.
Vanderhaeghen, W., Cerpentier T, Adriaensen C, Vicca J, Hermans K., Butaye, P. 2010. Methicillin-resistant Staphylococcus aureus (MRSA) ST398 associated with clinical and subclinical mastitis in Belgian cows. Vet. Microbiol. 144:166 -171. [DOI via Crossref]    [Pubmed]   
Wannet, W. J., Spalburg, E., Heck, M. E. 2004. Widespread dissemination in The Netherlands of the epidemic Berlin methicillin-resistant Staphylococcus aureus clone with low level resistance to oxacillin. J Clin. Microbiol. 42: 3077- 3082

This Article Cited By the following articles

Molecular Characterization of Zinc, Cobalt and Cadmium Resistance Genes of Staphylococcus aureus Detected in Sub-Clinical Cases of Mastitis in Cattle and She-Camel
J. Pure Appl. Microbiol. 2020; 14(1): 141.

1
 
Epidemiological studies based on multi-locus sequence typing genotype of methicillin susceptible <i>Staphylococcus aureus</i> isolated from camel’s milk
Onderstepoort J Vet Res 2017; 84(1): .

2
 
How to Cite this Article
Pubmed Style

Alsagher O. Ali. Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. AJVS. 2016; 48(1): 62-68. doi:10.5455/ajvs.210660


Web Style

Alsagher O. Ali. Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. http://www.alexjvs.com/?mno=210660 [Access: August 17, 2021]. doi:10.5455/ajvs.210660


AMA (American Medical Association) Style

Alsagher O. Ali. Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. AJVS. 2016; 48(1): 62-68. doi:10.5455/ajvs.210660



Vancouver/ICMJE Style

Alsagher O. Ali. Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. AJVS. (2016), [cited August 17, 2021]; 48(1): 62-68. doi:10.5455/ajvs.210660



Harvard Style

Alsagher O. Ali (2016) Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. AJVS, 48 (1), 62-68. doi:10.5455/ajvs.210660



Turabian Style

Alsagher O. Ali. 2016. Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. Alexandria Journal of Veterinary Sciences, 48 (1), 62-68. doi:10.5455/ajvs.210660



Chicago Style

Alsagher O. Ali. "Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt." Alexandria Journal of Veterinary Sciences 48 (2016), 62-68. doi:10.5455/ajvs.210660



MLA (The Modern Language Association) Style

Alsagher O. Ali. "Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt." Alexandria Journal of Veterinary Sciences 48.1 (2016), 62-68. Print. doi:10.5455/ajvs.210660



APA (American Psychological Association) Style

Alsagher O. Ali (2016) Molecular Epidemiology Based On SPA Genotyping Of Staphylococcus aureus Isolated From Cattle and Camels In Egypt. Alexandria Journal of Veterinary Sciences, 48 (1), 62-68. doi:10.5455/ajvs.210660





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]