E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal.

Abstract
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is expressed by numerous cells in the central nervous system. For determine the distribution of GFAP in brain tissues of normal and infected chicken and duck we conduct this study. A twenty-three 32-day-old male white Leghorn SPF chickens and twenty-three 32-day-old male commercial ducks were divided into control group (n= 5) and infected group (n= 18) from each bird type. 9a5b Newcastle disease virus (NDV) was used for bird infection and brain tissues from all groups were fixed in neutral buffered formalin and prepared for immunohistochemical investigation of GFAP expression. The expression of GFAP in control chickenís brain tissues is different than those of duck. While Bergmann-glia is GFAP-negative in control chickens; it appears positive in control ducks. GFAP expression in chickens is centered in the granular layer of cerebellum but in ducks it concentrated around the Purkinje cell layer. In the infected birds, numerous reactive astrocytes were observed in brain sections stained for GFAP in both chickens and ducks after 9a5b NDV infection. In chickens, GFAP-positive astrocytes were observed in the cerebellum, cerebrum, and midbrain as well as in the medulla oblongata. These changes were more prominent in areas of inflammatory response and neuronal loss. In turn, the brain tissues of infected duck express GFAP less than infected chickens. Duck brain tissues did not show any signs of inflammatory response, therefore, the expression of GFAP in duckís brain tissues still needs farther studies to explain the real causes underlying this expression.

Key words: GFAP; Astrocyte; Newcasle Disease Virus, Chickens; Ducks


 
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How to Cite this Article
Pubmed Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. AJVS. 2016; 48(2): 40-45. doi:10.5455/ajvs.216092


Web Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. http://www.alexjvs.com/?mno=216092 [Access: November 27, 2020]. doi:10.5455/ajvs.216092


AMA (American Medical Association) Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. AJVS. 2016; 48(2): 40-45. doi:10.5455/ajvs.216092



Vancouver/ICMJE Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. AJVS. (2016), [cited November 27, 2020]; 48(2): 40-45. doi:10.5455/ajvs.216092



Harvard Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal (2016) Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. AJVS, 48 (2), 40-45. doi:10.5455/ajvs.216092



Turabian Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. 2016. Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. Alexandria Journal of Veterinary Sciences, 48 (2), 40-45. doi:10.5455/ajvs.216092



Chicago Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. "Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck." Alexandria Journal of Veterinary Sciences 48 (2016), 40-45. doi:10.5455/ajvs.216092



MLA (The Modern Language Association) Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal. "Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck." Alexandria Journal of Veterinary Sciences 48.2 (2016), 40-45. Print. doi:10.5455/ajvs.216092



APA (American Psychological Association) Style

Anis Zaid, Safwat A.M. Ali, T. Morita, Sallah El Ballal (2016) Expression of Glial Fibrillary Acidic Protein in Brain Tissues after Experimental Infection with Newcastle Disease Virus: Comparative Study between Chicken and Duck. Alexandria Journal of Veterinary Sciences, 48 (2), 40-45. doi:10.5455/ajvs.216092





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