E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article
Online Published: 02 Aug 2016
 


Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland

Funmilola C. Thomas, Hayley Haining, Marion L. Stevenson, Hal Thompson, Ajibola E. Solomon, Peter D. Eckersall.


Abstract
Haptoglobin (Hp), an acute phase protein has been recognized as an important indicator of intramammary infections that can be assessed in milk. In this study, we utilized immunohistochemical techniques on naturally infected and healthy bovine mammary glands in order to determine the source of Hp found in milk during bovine mastitis. Mammary glands sections were harvested from udder of two cows, one showing signs of clinical mastitis and the other healthy. Hematoxylin and eosin as well as immunohistochemistry (IHC) for anti-Hp staining were carried on these sections. It was revealed that the mammary epithelial cells around the alveolar ducts of the gland as well as with infiltrating neutrophils had dense staining for Hp in mastitic sections while in the healthy section there was minimal cytoplasmic positivity for Haptoglobin on the ductal epithelial cells. These observations support the conclusion that Hp arises locally from mammary epithelial cells and from circulation from migrating neutrophils during episodes of mastitis, and can therefore serve as a sensitive indicator of bovine mastitis.

Key words: Haptoglobin, Bovine, Mammary gland, Immunohistochemistry, mastitis


 
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How to Cite this Article
Pubmed Style

Thomas FC, Haining H, Stevenson ML, Thompson H, Solomon AE, Eckersall PD. Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. AJVS. 2016; 50(1): 65-69. doi:10.5455/ajvs.218223

Web Style

Thomas FC, Haining H, Stevenson ML, Thompson H, Solomon AE, Eckersall PD. Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. https://www.alexjvs.com/?mno=218223 [Access: May 04, 2025]. doi:10.5455/ajvs.218223

AMA (American Medical Association) Style

Thomas FC, Haining H, Stevenson ML, Thompson H, Solomon AE, Eckersall PD. Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. AJVS. 2016; 50(1): 65-69. doi:10.5455/ajvs.218223


Vancouver/ICMJE Style

Thomas FC, Haining H, Stevenson ML, Thompson H, Solomon AE, Eckersall PD. Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. AJVS. (2016), [cited May 04, 2025]; 50(1): 65-69. doi:10.5455/ajvs.218223


Harvard Style

Thomas, F. C., Haining, . H., Stevenson, . M. L., Thompson, . H., Solomon, . A. E. & Eckersall, . P. D. (2016) Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. AJVS, 50 (1), 65-69. doi:10.5455/ajvs.218223


Turabian Style

Thomas, Funmilola C., Hayley Haining, Marion L. Stevenson, Hal Thompson, Ajibola E. Solomon, and Peter D. Eckersall. 2016. Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. Alexandria Journal of Veterinary Sciences, 50 (1), 65-69. doi:10.5455/ajvs.218223


Chicago Style

Thomas, Funmilola C., Hayley Haining, Marion L. Stevenson, Hal Thompson, Ajibola E. Solomon, and Peter D. Eckersall. "Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland." Alexandria Journal of Veterinary Sciences 50 (2016), 65-69. doi:10.5455/ajvs.218223


MLA (The Modern Language Association) Style

Thomas, Funmilola C., Hayley Haining, Marion L. Stevenson, Hal Thompson, Ajibola E. Solomon, and Peter D. Eckersall. "Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland." Alexandria Journal of Veterinary Sciences 50.1 (2016), 65-69. Print. doi:10.5455/ajvs.218223


APA (American Psychological Association) Style

Thomas, F. C., Haining, . H., Stevenson, . M. L., Thompson, . H., Solomon, . A. E. & Eckersall, . P. D. (2016) Immunohistochemical localization of Haptoglobin in the Bovine Mammary Gland. Alexandria Journal of Veterinary Sciences, 50 (1), 65-69. doi:10.5455/ajvs.218223




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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