E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh.

Abstract
The present study was intended to prepare and evaluate the applicability of fluorescein-conjugated anti-RHDV polyclonal antibodies for routine diagnosis and research purposes. A total of 22 animals (10 rabbits, 10 guinea pigs and 2 goats) were immunized subcutaneously with locally prepared RHDV inactivated vaccine containing 210 HAU per ml (rabbits and guinea pigs received 0.5 ml per dose, mean while goats received 1ml). Five doses with 7 days intervals were applied. The last dose was simultaneously injected with Montanide¬ô ISA 206 oil adjuvant. Five weeks post-immunization, RHDV-specific HI antibody titers were significantly increased and reached to the peak level with a mean titer of 7, 8 and 8.5 log2 in guinea pig, rabbit and goat sera, respectively. The globulin fraction of the prepared antisera was calculated, successfully precipitated with ammonium sulphate and then conjugated with FITC. The application of direct IF test on impression smears prepared from tissues of experimentally infected rabbits with RHDV isolate Giza-2006, revealed that positive reactions were obtained with guinea pig, rabbit and goat conjugates up to a dilution of 10-4, 10-5 and 10-6respectively. These findings clearly indicate that fluorescein-conjugated polyclonal antibodies developed in the present study can be used for accurate and precise diagnosis of RHDV.

Key words: Rabbit haemorrhagic disease virus (RHDV), Polyclonal antibodies, immunofluorescence, conjugate


 
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How to Cite this Article
Pubmed Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. 2016; 51(2): 46-51. doi:10.5455/ajvs.219728


Web Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. https://www.alexjvs.com/?mno=219728 [Access: December 07, 2023]. doi:10.5455/ajvs.219728


AMA (American Medical Association) Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. 2016; 51(2): 46-51. doi:10.5455/ajvs.219728



Vancouver/ICMJE Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS. (2016), [cited December 07, 2023]; 51(2): 46-51. doi:10.5455/ajvs.219728



Harvard Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh (2016) Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. AJVS, 51 (2), 46-51. doi:10.5455/ajvs.219728



Turabian Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. 2016. Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 46-51. doi:10.5455/ajvs.219728



Chicago Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. "Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus." Alexandria Journal of Veterinary Sciences 51 (2016), 46-51. doi:10.5455/ajvs.219728



MLA (The Modern Language Association) Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh. "Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus." Alexandria Journal of Veterinary Sciences 51.2 (2016), 46-51. Print. doi:10.5455/ajvs.219728



APA (American Psychological Association) Style

Basma A. Elkady, Emad M. Al-Ebshahy, Samy A. Khalil, Amani A. Saleh (2016) Development of Fluorescein-Labelled Antisera for Rapid Detection of Rabbit Haemorrhagic Disease Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 46-51. doi:10.5455/ajvs.219728





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