Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources

Ayman M. Kamar; Helmy A. Torky; Samia M. El-Houshy

doi:10.5455/ajvs.222088

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Original Article

AJVS. 2016; 51(2): 148-157

doi: 10.5455/ajvs.222088

Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources

Ayman M. Kamar, Helmy A. Torky, Samia M. El-houshy.

Abstract
The aim of this study was to determine the differences concerning phenotypic and genotypic characters of L.monocytogenes isolated from different sources (100 meat, 100 offals, 100 fish and 100 ready to eat foods -RTE- which is represented by luncheon) on the basis of pathogenicity and antibiotic resistance. To achieve this aim, bacteriological examination was done according to ISO 11290 amendment 2004.then confirmed by biochemical reactions and serotyping, then antimicrobial susceptibility testing which indicates the presence of multidrug resistance isolates. Biofilm binding activity of the isolated L.monocytogenes isolates showed that two isolates had strong binding activity, three isolates showed moderate binding activity and one isolate was weak/non biofilm producer. The molecular characterization was done by detection of class1 integron and for pathogenicity by detection of the presence of hlyA gene which encodes listeriolysin of L.monocytogenes then sequencing of the resulted PCR product to determine the difference between the isolates. The confirmation of results was done by SYBR green Real-time PCR.

Key words: Listeria monocytogenes, sequencing, conventional PCR, Realtime PCR, serotyping, biofilm, antimicrobial susceptibility

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Pubmed Style

Kamar AM, Torky HA, El-houshy SM. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. 2016; 51(2): 148-157. doi:10.5455/ajvs.222088

Web Style

Kamar AM, Torky HA, El-houshy SM. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. https://www.alexjvs.com/?mno=222088 [Access: May 04, 2025]. doi:10.5455/ajvs.222088

AMA (American Medical Association) Style

Kamar AM, Torky HA, El-houshy SM. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. 2016; 51(2): 148-157. doi:10.5455/ajvs.222088

Vancouver/ICMJE Style

Kamar AM, Torky HA, El-houshy SM. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. (2016), [cited May 04, 2025]; 51(2): 148-157. doi:10.5455/ajvs.222088

Harvard Style

Kamar, A. M., Torky, . H. A. & El-houshy, . S. M. (2016) Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS, 51 (2), 148-157. doi:10.5455/ajvs.222088

Turabian Style

Kamar, Ayman M., Helmy A. Torky, and Samia M. El-houshy. 2016. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 51 (2), 148-157. doi:10.5455/ajvs.222088

Chicago Style

Kamar, Ayman M., Helmy A. Torky, and Samia M. El-houshy. "Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 51 (2016), 148-157. doi:10.5455/ajvs.222088

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Kamar, A. M., Torky, . H. A. & El-houshy, . S. M. (2016) Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 51 (2), 148-157. doi:10.5455/ajvs.222088

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