E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels

Maha A. Ibrahim, Howida M. A. Abd-El-Rahman, Rawash. Z. M and Abeer, E. El-Metwally.

Abstract
These findings could give a spot light on the possible links between biochemical, hormonal and histopathological changes and their impact on male camel seasonal infertility. Seventy four dromedary camel bulls were used during rutting and non-rutting season. Serum and epididymal fluid analysis for creatine kinase (CK), lactate dehydrogenase (LDH), glutathione peroxidase (GPX), total testosterone (T), tri-iodothyronine (T3) and thyroxine (T4) hormones were estimated. Evaluation of epididymal spermatozoa and histopathological examination associated with histomorphometric analysis of the tissue samples were done. Serum and epididymal fluid GPX, CK and LDH, T level were significantly high (P>0.05) in rutting season. Meanwhile, thyroid hormones (T3 and T4) levels increased significantly (P < 0.05) in the rutting than the non-rutting season in serum only. Motility and livability of camel epididymal spermatozoa collected during rutting season were significantly (P< 0.05) higher than in non-rutting season. Morphologically abnormal spermatozoa and those with abnormal acrosomes in breeding season were significantly (P < 0.05) lower than non-breeding season. Histopathological and histomorphometric analysis of camel testes and epididymis indicated various patho-physiological conditions including chronic orchitis and/or epididymitis, various degrees of degeneration, necrosis, atrophy and hyperplasia of leydig cells associated with disturbance in spermatogenesis were reported in rutting and non -rutting seasons. Furthermore, the average height of the seminiferous epithelium layer, thickness of propria tunica and tubular diameter were estimated and the proportion of seminiferous tubules, interstitial tissue, lymphatic vessels and blood vessels of each camel testicle were recorded. Elevation of the ambient temperature during non- rutting season seemed to play the main role in camel reproductive activities through disturbance of the physiological activities so, the most of testicular changes might be reversible and transient except severe pathological alterations. The evaluation of biochemical and hormonal parameters in serum may consider some what a reflection to their levels in epididymal fluid that could be a new biomarker of gonadal function and reproductive performance in camels.

Key words: male, dromedary camel, CK, LDH, GPX, Testosterone, Thyroid, seminal analysis, histopathology, histomorphometry.


 
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REFERENCES
Abbot, B.1984.Creatine Kinase. Textbook of Clinical Chemistry, 3rd ed, 1112-1116.
Abdalla, E.B., Zeidan, A.E.B.; Abd El-Salaam, A.M. ,Maiada W.A. 2011.Thermoregulation and blood biochemical changes in male Dromedary camel during hot-humid and hot-dray environments under Egyptian condition. J. Camel Practice and Research. Vol. 18(2): 297-304.
Abd El-Azim, A.M.1996.Aging and its effect on the reproductive performance of male one- humped camel during different seasons. Ph.D. Thesis, Fac. Vet. Med., Zagazig Univ., Zagazig, Egypt.
Abd-Elaziz, M. I., Kassem, A. M.; Zaghloul, D. M., Derbalah, A. E.,Bolefa, M. H. 2012.Ultrastructure of the Interstitial Tissue in the Testis of the Egyptian Dromedary Camel (Camelus dromedarius). Pak. Vet. J, 32(1): 65-69.
Abdel Rahim A.G 2005. The relationship between whole blood selenium (Se) concentration and the activity of the seleno-enzyme, glutathione peroxidase (GSH-Px, E. I.11.1.9) in camel (Camelus dromedarius). J. Arid. Env. , 62, 359-362.
Abd El-Samee, A.M., Zeidan, A.E.B.; Abbas, H.E., Ahmadi, E.A.A. 2006. Semen quality and testicular histology with special reference to different seasons of the year and ages of the male Dromedary camel. Proc. Intern. Scientific Conf. Camels, 10-12 May, Qassim Univ., K.S.A.
Agarwal, V.K., Ram, L.; Rai, A.K.; Khanna, N.D. , Agarwal, S.P. 2004. Physical and biochemical attributes of camel semen. J. Camel Sci., 1: 25-30.
Agarwal, S.P 2005. Oxidative stress and antioxidants in male infertility: a difficult balance, Review article, Iran. J. of Reprod. Med. (3)1: 1-8.
Agarwal, S.P., Rai, A.K., Khanna, N.D., 1991. Effect of mating on hormone levels in male camels. Indian Vet. J. l (68): 931-933.
Ahmadi, E.A.A. 2001. Physiological and reproductive studies on camels. Ph.D. Thesis, Fac. Agric., Zagazig Univ., Zagazig, Egypt.
Ali A., Najlaa S. Ibrahim 2014. Effect of age and season on the Epididymal Sperm and testosterone level in camel (camelus dromedarius). The Iraq. J. of Vet. Med., 38(1): 24 -29.
Almeida, O.F., Lincoln, G.A. 1984. Reproductive retractors in rams and accompanying changes in the patterns of melatonin and prolactin secretion. Biol. Reprod., 30: 143-154.
Alkaladi A, Mohamed A, Yahia Y M, Elbeshehy E.K.F 2013. Levels of Reduced Glutathione in Erythrocyte of different Arabian camel (Camelus dromedaries) Breeds, Life Sci. J. 10(4): 130-134.
Alsumait, A. A. 2012. The role of testicular biobsy and ultrasonography in evaluation of reproductive dysfunction in camels (Camelus dromedarius). M. V. Sc. thesis, Kingdom of Saudi Arabia.
Al-Qarawi A.A., Omar H.M., Abdel-Rahman H.A., ElMougy S.A., El-Belely M.S 2004. Trypanosomiasisinduced infertility in dromedary (Camelus dromedarius) bulls: changes in plasma steroids concentration and semen characteristics. Anim. Reprod. Sci.; 84:73-82.
Bancroft, J.D., Marilyn G, 2012: Theory and practice of histological techniques. 5th London Edinburgh New York Philadelphia St. Louis Sydney Toronto.
Beckman K. B., Ames B. N 1998.The free radical theory of ageing matures. Physio. Rev. 78:547-581.
Braverman, L.E., Ingar, S.H. 1969. Clin. Res.17: 458Diaconescu, C., Matei, M., T˘alpu, G., T˘ap˘aloag˘a, P 2014. Comparative physicochemical and biochemical characterization of bull and boar semen. Sci. Pap. Ser., D: Anim. Sci. 141-145.
Burgos, C., Maldonado, C., Gerezde Burgos, N.M., Aoki, A. ,Blanco, A. 1995.Intracellular localization of the testicular and sperm-specific lactate dehydrogenase isoenzyme C4 in mice. Biol. Reprod.; 53:84-92. [DOI via Crossref]    [Pubmed]   
Chellman, G.J., Bus, J.S., Working, P.K. 1986.Role of epididymal inflammation in the induction of dominant lethal mutations in Fischer 344 rat sperm by methyl chloride. Proc. Natl. Acad. Sci. USA 83: 8087-8091.
Chen, Angela S. P, June L, Matthew C. B, Terry R. B,Barry R. Z 2008.Effect of Glutathione Depletion on Leydig Cell Steroidogenesis in Young and Old Brown Norway Rats Haolin Endo. 149(5):2612-261.
Chen, A., Bookstein, J.J., Meldrum, D. R.1991. Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertility and Sterility J; 55:1202-1203. [DOI via Crossref]   
Clegg, E.D., Cook, J.C., Chapin, R.E., Foster, P.M.D., Daston, G.P.1997. Leydig cell hyperplasia and adenoma formation: Mechanisms and relevance to humans. Reprod. Toxic. 11: 107-121.
Costa, F.L.A., Silva .S.M.M.S. and Nascimento, E.F. 2007. Pathologic evaluation of testis and epididymis of hairy rams in the semi-arid region of Piaui state. Arq. Bras. Med. Vet. Zootec. 59: 1110-1116.
Da Silva, K. M.,Zart, A. L.,Brum, K.B., Fernandes, C.E.F. 2015.Histopathological and histomorphometric testicular characteristics associated to reproductive condition in Bosindicus (Nellore) bulls.
Dandekar, S.P., Parkar, G.M.1999. Correlation between creatine kinase activity, lipid-peroxidation and water test in male infertility, Department of Biochemistry, Seth G.S. Medical College India, 45:42-48.
Deen, A. 2008. Testosterone profiles and their correlation with sexual libido in male camels. Res Vet Sci 2008, 85: 220-226.
Dellmann, H.D., Eurell, J.A.1998.A Textbook of Veterinary Histology. 5th ed., Williams and Wilkins, a Waverly Company, Philadelphia, USA. pp. 226-235.
Diaconescu, C., Matei, M., T˘alpu, G., T˘ap˘aloag˘a, P 2014.Comparativephysicochemical and biochemical characterization of bull and boar semen. Sci. Pap. Ser., D: Anim. Sci. 141-145.
Eiler, H. and Armstrong-Backus, C. S. 1987. Passage of exogenous L-thyroxine and triiodothyronine into bovine ejaculate. Amer. J. Vet. Res. 48, 429-443.
El-Kon, I.I.; Heleil, B.A., Mahmoud, S.A. 2011.Effect of age and season on the testicular sperm reserve and testosterone profile in camel (camelus dromedarius). Anim. Reprod, 8: 68-72.
Eva, T., KŇAŽICKÁ, Z., BÁRDOS, L., MASSÁNYI, P. and LUKÁČ, N.2011. Impact of oxidative stress on male fertility. Act. Vet. Hung. 59(4): 465.
Foster, R. A. 2012. Male reproductive system: in pathologic basis of veterinary disease,” J. F. Zachary and M. D. Mc Gavin, Eds., pp. 1127-1152, Mosby University Graphics Production, Missouri, Mo, USA,5th ed.
Foster, R. A., Ladds, P. W. 2007. Male genital system. In: MAXIE, M. G. Jubb, Kenndey and Palmer´s. pathology of domestic animals. 5th ed. Philadelphia: Elsevier, 3:565-619.
Friedlander, M., Rosen strauch, A. , Bedrak, E., 1984. Leydig cell differentiation during the reproductive cycle of the seasonal breeder Camelus dromedarius: an ultrastructural analysis. Gen. Comp. Endo., 55: 1-11.
Giannattasio, A., De Rosa, M., Smeraglia, R., Zarrilli, S., Cimmino, A., Di Rosario, B., Ruggiero, R., Colao, A. , Lombardi, G.2002. Glutathione peroxidase (GPX) activity in seminal plasma of healthy and infertile males, J. Endo. Invest. 25(11):983-6.
Gu¨ndog˘un M. 2006. Some reproductive parameters and epididymal fluid constituents in relation to season in Akkaraman and Awassi rams. Turk. J. Vet. Anim. Sci., 30:95-100.
Hallak Jorge, Rakesh K. Sharma, Fabio F. Pasqualotto, Pavithra Ranganathan, Anthony J. Thomas, Jr, Ashok Agarw 2001.Creatine Kinase As An Indicator Of Sperm Quality and Maturity In Men With Oligospermia. Adult Urology Cme, Article Urology 58 (3): 447-451
Hemeida, N.A., El-Wishy, A.B., Ismail, S.T.1985.Testicular abnormalities in the one-humped camel. In: Proceedings of the 1st Int. Conf. Appl. Sci., Zagazig University, Egypt, 2:438-449.
Huszar,G. and Vigue,L. 1993.Incomplete development of human spermatozoa associated with increased creatine phosphokinase concentration and abnormal head morphology. Mol. Reprod. Dev.1993:34:292-298. [DOI via Crossref]    [Pubmed]   
Igbokwe, I. O., Ikpo, A. E., Grema, H.A., Mshelbwala, F.M. and Igbokwe, N. A.2011.Bilateral testicular hypoplasia among mature Sahel bucks in Nigeria. Turk. J. Vet. Anim. Sci. (35)2:111-115.
Inan, M., Basaran, U.N., Dokmeci, D., Yalcin, O., Aydogdu, N. , Turan, N. 2008. Methylene blue increases contralateral testicular ischemia-reperfusion injury after unilateral testicular torsion. Clin. Exp. Pharmacol. Physiol., 35(1):50-4.
Javed, M.T., Khan, A. Naz, N. A. 2001.Studies on abnormal buffalo bulls with reference to scrotal circumference, semen characteristics, epididymal fluid hormones and their association with testicular and epididimal histopathology. Vet. Ski. Arch. 71(4): 223-236.
Jeyendran, R. S., Vander-Ven, H.H., Perez-Pelaez, M., Crabo, B. G., Zanevld, L.J.D. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. J. Reprod. Fertil. 70: 219-228.
Johnson, L. and Thompson, D. L. 1987.Effect of seasonal changes in Leydig cell number on the volume of smooth endoplasmic reticulum in Leydig cells and intra testicular testosterone content in stallions. J. Reprod. Fertil. 81:227-232. [DOI via Crossref]    [Pubmed]   
Jones, A.R. 1997.Metabolism of lactate by mature boar spermatozoa. Reprod. Fertil. Dev.; 9:227-232. [DOI via Crossref]    [Pubmed]   
Kaneko, J.J. 2008.Thyroid function. In: Kaneko JJ, Harvey JW, Bruss ML (Eds.). Clinical biochemistry of domestic animals. 6thed. New York, USA: Academic Press Inc. 627-630.
Kareskoski, M., Katila, T. 2008. Components of stallion epididymal fluid and the effects of epididymal fluid on sperm longevity. Anim. Reprod. Sci., 107:249-256. [DOI via Crossref]    [Pubmed]   
Khan, A.A. 1971. Sexual behavior of the male camel (Camelus dromedaries) and studies on semen. M. V.Sc. Thesis, Bikaner Univ., Udaipur, India.
Khanna, D., Agarwal, S.P., Gupta, M.L., Rai, A.K., Khanna, N.D. , Khanna, D.1996.Effect of water deprivation during summer and winter on thyroid hormones concentration in the Indian camel. Indian J. Anim. Sci. 66:253-255.
Kumar, P., Singh, I., Yadac PS. 2012.Epididymal fluid proteome. Promising biomarkers for bull fertility. Agri. Res.; 1:78-86.
Landau, B., Singer, R., Shindel, B., Laor, J., Sagiv, M. , Barnet, M.1983. Triiodothyronine and tetra iodothyronine in human semen prior to, and following, treatment with thyroid extracts. Experientia 39, 544-545.
Lasota, B., Błaszczyk, B., Stankiewicz,T., Udała,J., Szewczyk, M. , Matusiak- Bielska ,D. 2009. Super oxide dismutase and glutathione peroxidase activity in porcine follicular fluid in relation to follicular size. Acta. Sci. Pol. Zoo technical. 8, 19-30.
Lynda L. Lanning, Creasy, D. M., Chapin R. E., Mann,P. C., Barlow, N. J., Regan, K. S. and Dawng, G. 2002.Recommended approaches for the evaluation of testicular and epididimal toxicity. Toxic. Path., 30, 4: 507-520.
Micklem, K., Sandersons, J.2001. Digital imaging in pathology. Current Diagnostic Pathology, Sheffield, 7(2): 131-140. [DOI via Crossref]   
Migbaru, K., Sisay, G. and Kasa, T. 2014. Study on Gross Testicular Disorders of Bulls Slaughtered at Addis Ababa Abattoirs Enterprise. J. Reprod. Infer. 5 (2): 45-49.
Moosa-Ali, S., Barati, F., Esmaeilzadeh, S., Gharibi, D. , Khaksary, M. M. 2014. Testicular lesions and epididymal sperm parameters in the Iranian river buffalo (Bubalusbubalis). Andrologia, 24.
Morone, S.; Grim, J.M., Simonik, E.A., Semones, M.C., Kuhn, D.E., Crockett, E.L.2013. The glutathione-dependent system of antioxidant defense is not modulated by temperature acclimation in muscle tissues from striped bass. Comp Bio. Physiol. A Mol. Integr. Physiol. 164(2):383-90.
Nascimento, E. F., Maia, P. C.C. and Chiquiloff, M. A G. 1981. Alterações testiculares e epididimárias em bovinos: II Degeneração e cistos intraepiteliais no epidídimo. Arq. Esc. Vet. UFMG, 33:407-412.
Nazifi, S., Nikahval, B. and Mansourian, M. 2009. Relationships between thyroid hormones, serum lipid profile and erythrocyte antioxidant enzymes in clinically healthy camel (Camelus dromedarius). Revue. Med. Vet., 160 (1): 3-9.
Olayinka, E. T. and Ore, A. 2015.Hepatotoxicity, nephrotoxicity and oxidative stress in rat testis following exposure to haloxyfop-p-methyl ester, an aryloxyphenoxypropionate Herbicide. Toxics, 3: 373-389.
Pesce A. 1984. Lactate dehydrogenase. Kaplan A. et al. Clin. Chem. The C.V. Mosby Co. St. Louis. Toronto. Princeton. 1124-1127.
Pasha, R.H., Qureshi, A.S. and Khanum, S.A. 2015. Seasonal variations in the sex-steroid hormones and behavior of one humped male camel (Camelus dromedarius) in Punjab, Pakistan. J Vet., 3 (1) 26-32.
Paglia, D.E. and Valentine, W.N.1967. J. Lab. Clin. Med.70:158-169.
Pesce A. 1984. Lactate dehydrogenase. Kaplan A. et al. Clin. Chem. The C.V. Mosby Co. St. Louis. Toronto. Princeton. 1124-1127.
Pourjafar, M., Chalmeh, A.,Badiei, Kh., Nazifi, S., Keshavarz, S. and Naghib,M. 2013. Correlations among homocysteine, cardiac troponin I and cardiac enzymes in different ages of clinically healthy male Dromedary camels. Shiraz, Iran. J. Vet. Med.7 (3):201-206.
Plasson, S.J. 1975. Veterinary Obstetries and Gynecology. William and Wilson Company, 4th (Ed.), New York, USA, pp. 415-486.
Prakash, P., Rathore, V.S. 1991. Seasonal variations in blood serum profiles of triiodothyronine and thyroxine in goat. Indian J Anim. Sci. 61(12): 1311-1312.
Rateb, S.A., Zaglol, A.A., M.A. Khalifa and El-Bahrawy, K.A. 2012. Int. Workshop on Recent Strategies in Animal Production, Alexandria, Recent Advances and Modern Assisted Reproductive Technologies in Camels A Review, Division of Animal and Poultry Production, Desert Research Center, Ministry of Agriculture and Land reclamation, Egypt.
Rayamajhi Manira, Yue Zhang ,Edward Miao 2013. Detection of pyroptosis by measuring released lactate dehydrogenase activity. Methods Mol. Biol., 1040: 85-90.
Rui-Xiang Feng, Jin-Chun Lu, Hong-Ye Zhang, and Nian-Qing Lü 2015. A Pilot Comparative Study of 26 Biochemical Markers in Epididymal fluid and Serum in Infertile Men, Bio Med Res. Inter., Article ID 805328, 7 pages.
Salisbury, G.W., Van Demark, N.L. and Lodge, J.R. 1978. Physiology of Reproduction and Artificial Insemination of Cattle. WH Freeman and Company, San Francisco, USA.
Sekoni, V.O., Rekwot, P.I. , Bawa, E.K. and Barje, P.P.2010. Effect of age and time of sampling on serum testosterone and spermiogram of Bunaji and N’Dama bulls. Res. J. Vet. Sci. 3(1): 62-67.
Setchell, B. P. and Breed, W. G. 2006. Anatomy, vasculature, and innervation of the male reproductive tract. In.: NEILL, J. D. Knobil and neill’s physiology of reproduction. 3th ed. Philadelphia: Elsevier: 771-825.
Sidhu, R.S., Sharma, R.K. , Agarwal, A. 1998. Relationship between creatine kinase activity and semen characteristics in subfertile men. Int J Fertil Womens Med. 43(4):192-7.
Sinha, H.S. , Prasad, R.B. 1993. Seasonal variation in semen character. Indian Dairy Sci., 19: 83-85.
Skidmore, J.A. 2003.The main Challenges Facing Camel Research in the 21 st. Century. Reprod. Suppl., 61:37-47.
Sirat, M.P., Sinha, A.K., Singh, B.K. and Prasad R.L.1996. Effects of cryoprotectants on release of various enzymes from buck spermatozoa during freezing, Theriogenology, 45:405-416.
Snedecor, G.W. and Cochran, W.G. 1982. Statistical methods. 7th Edition, the Iowa State University Press, Iowa.
Tajik, J., Sazmand, A., Moghaddam, S.H.H., Rasooli,A. 2013. Serum concentrations of thyroid hormones, cholesterol and triglyceride, and their correlations together in clinically healthy camels (Camelus dromedarius): Effects of season, sex and age. Vet. Res. Forum. 4 (4) 239 - 243.
Tanýdýr, Y., Gümrah,A.; Akbal, C. and Turcan, T. 2008. Brucella epididymo-orchitis as the first presenting sign of Brucellosis: a case report and review of the literature. Marmara Med. J., 21:56-60.
Tavilani, H., Goodarzi, M.T., Vaisi-raygani, A. , Salimi S, Hassanzadeh, 2008.Activity of antioxidant enzymes in epididymal fluid and their relationship with lipid peroxidation of spermatozoa. Int. Braz. J. Urol., 34(4):485-91.
Teixeira, A.M. and Borges, G.F. 2012. Creatine kinase: structure and function. Braz. J. Biomotricity 6, 53-65.
Tibary, A., Anouassi, A. 2000.Reproductive Disorders in the Male Camelid. In: Recent Advances in Camelid Reproduction, Skidmore J.A. and Adams G.P. (Eds.) Publisher: International Veterinary Information Service.
Tombes, R.M. and Shapiro, B.M.1995. Metabolite channeling. A phosphoryl creatine shuttle to mediate high energy phosphate transport between sperm mitochondrion and tail. Cell, 41:325-334.
Volkmann, J., Muller, D., Feuerstacke, C., Kliesch, S., Bergmann, M., Muhlfeld C. and Middendorff, R. 2011. Disturbed spermatogenesis associated with thickened lamina propria of seminiferous tubules is not caused by dedifferentiation of myofibroblasts. Hum. Reprod. , Child. 26(6): 1450-1461.
Volodymyr, I. Lushchak, 2012. Glutathione Homeostasis and Functions: Potential Targets for Medical Interventions. J. Amin. Acid. 2012:1-26.
Waheed, M.M., Ghoneim, I.M , Alhaider, A.K.2015.Epididymal fluid and serum fertility biomarkers in Dromedary camels (Camelus dromedarius). Theriogenology. 1, 83(4):650-4.
Washington, Ida M, Gerald Van. Hoosier 2012. In the Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents Pp. 57-116. Boston: Academic Press Chapter 3 - Clinical Biochemistry and Hematology.
Watson, P.F. 1975. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet. Rec., 97: 12-15.
Wai-sum, O., Chen, H., Chow, P.H., 2006. Male genital tract antioxidant enzymes - their ability to preserve sperm DNA integrity. Mol. Cell. Endo. 250, 80-83.
Wistom, G.B.1976. EnzymeImmunoassy.Clin.Chem.22:1243-1243.
Yagil, R., Etzion, Z., Gannani, J., 1978. Camel thyroid metabolism: effect of season and dehydration. J. Appl. Physiol.45, 540-544.
Zeidan, A.E.B., Abbas, H.E. 2004. Physiological and biochemical changes in the male Dromedary camels during rutting and non-breeding seasons. Zagazig Vet. J., 32: 37-48
Zeidan, A.E.B., Habeeb, E.A.M., Ahmadi, E.A., Amer, H.A., Abd El-Razik, M.A. 2001. Testicular and physiological changes of the male Dromedary camels in relation to different ages and seasons of the year. Proc. 2nd Intern. Conf. Anim. Prod. Health in Semi-Arid Areas, El-Arish North Sinai, Egypt, 147-160.
Zia-ur-Rahman, A.N., Shazia, A.B., Akhtar, N. 2007. Serum hormonal, electrolytes and trace element profiles in the rutting and non-rutting one-humped male camel (Camelus dromedarius). Anim. Reprod. Sci. 101(1):172-178.

How to Cite this Article
Pubmed Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . AJVS. 2016; 49(2): 189-202. doi:10.5455/ajvs.226663


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Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . http://www.alexjvs.com/?mno=231517 [Access: August 18, 2021]. doi:10.5455/ajvs.226663


AMA (American Medical Association) Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . AJVS. 2016; 49(2): 189-202. doi:10.5455/ajvs.226663



Vancouver/ICMJE Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . AJVS. (2016), [cited August 18, 2021]; 49(2): 189-202. doi:10.5455/ajvs.226663



Harvard Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally (2016) Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . AJVS, 49 (2), 189-202. doi:10.5455/ajvs.226663



Turabian Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. 2016. Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . Alexandria Journal of Veterinary Sciences, 49 (2), 189-202. doi:10.5455/ajvs.226663



Chicago Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. "Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels ." Alexandria Journal of Veterinary Sciences 49 (2016), 189-202. doi:10.5455/ajvs.226663



MLA (The Modern Language Association) Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally. "Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels ." Alexandria Journal of Veterinary Sciences 49.2 (2016), 189-202. Print. doi:10.5455/ajvs.226663



APA (American Psychological Association) Style

Maha A. Ibrahim , Howida M. A. Abd-El-Rahman , Rawash. Z. M and Abeer, E. El-Metwally (2016) Studies on Some Biochemical, Hormonal, Histopathological and Seminal Characters in Relation to Rutting and Non-Rutting Season in Camels . Alexandria Journal of Veterinary Sciences, 49 (2), 189-202. doi:10.5455/ajvs.226663





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