E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
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Original Article 


Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens

May K. Zaghloul, Emad M. Al-ebshahy, Samy A. Khaliel, Hany F. Ellakany.


Abstract
The present study was aimed to investigate the prevalence of multidrug resistant S. enteritidis in broiler chicken flocks (n=15)suffering from diarrhea during the period of 2015 in El-Beheira governorate. A total of 210 tissue swabs (liver, intestine, spleen and gall bladder) were aseptically collected, processed and plated onto XLD agar medium. Only five (2.4%) isolates were identified as Salmonella using conventional biochemical methods and PCR assay based on invA gene. Out of 5 Salmonella isolates, 4 (80%) were identified as S. enteritidis using PCR assay based on sefA gene.Antimicrobial susceptibility testing revealed that all isolates of S. enteritidis were multi-resistant to two or more antimicrobial agents. Resistance tosulfisoxazole was common across all isolates. Furthermore, two (50%) penicillin-resistant isolates were tested positive for blaTEM gene using PCR assay. In addition, β-lactamase activity of these isolates was assessed on penicillin-sensitive strain of S. aureus (ATCC29213). Sensitivity of S. aureus to penicillin was significantly decreased in the presence of beta-lactamase-producing S.enteritidis in comparison with control group. The current study necessitates further exploration of genetic elements related to antibiotic resistance and underlines the need to track the evolution of β-lactamases in S. entritidis isolates in Egypt.

Key words: Key words:Salmonella enteritidis, β-lactamase, PCR, Egypt.


 
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How to Cite this Article
Pubmed Style

Zaghloul MK, Al-ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. 2017; 55(1): 204-209. doi:10.5455/ajvs.246419

Web Style

Zaghloul MK, Al-ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. https://www.alexjvs.com/?mno=246419 [Access: March 25, 2025]. doi:10.5455/ajvs.246419

AMA (American Medical Association) Style

Zaghloul MK, Al-ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. 2017; 55(1): 204-209. doi:10.5455/ajvs.246419


Vancouver/ICMJE Style

Zaghloul MK, Al-ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. (2017), [cited March 25, 2025]; 55(1): 204-209. doi:10.5455/ajvs.246419


Harvard Style

Zaghloul, M. K., Al-ebshahy, . E. M., Khaliel, . S. A. & Ellakany, . H. F. (2017) Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS, 55 (1), 204-209. doi:10.5455/ajvs.246419


Turabian Style

Zaghloul, May K., Emad M. Al-ebshahy, Samy A. Khaliel, and Hany F. Ellakany. 2017. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. Alexandria Journal of Veterinary Sciences, 55 (1), 204-209. doi:10.5455/ajvs.246419


Chicago Style

Zaghloul, May K., Emad M. Al-ebshahy, Samy A. Khaliel, and Hany F. Ellakany. "Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens." Alexandria Journal of Veterinary Sciences 55 (2017), 204-209. doi:10.5455/ajvs.246419


MLA (The Modern Language Association) Style

Zaghloul, May K., Emad M. Al-ebshahy, Samy A. Khaliel, and Hany F. Ellakany. "Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens." Alexandria Journal of Veterinary Sciences 55.1 (2017), 204-209. Print. doi:10.5455/ajvs.246419


APA (American Psychological Association) Style

Zaghloul, M. K., Al-ebshahy, . E. M., Khaliel, . S. A. & Ellakany, . H. F. (2017) Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. Alexandria Journal of Veterinary Sciences, 55 (1), 204-209. doi:10.5455/ajvs.246419




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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