E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
80 / 1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta.

Abstract
A total of 543 sera from unvaccinated ruminant species, viz. cows, buffalo cows, ewes, female goats and she-camels in seven Nile Delta governorates. Most animals had a history of Brucella melitensis biovar 3 infection. This study was to investigate the fitness of Rose-Bengal plate test (RBPT) formats namely the US brucellosis card (BCT) 8% and 3%, the UK RBPT and its modification, the Scottish RBPT and the French RBPT 4.5% for pre-confirmation of B. melitensis infection in non-bovine ruminants. Taking the complement fixation test (CFT) as the gold standard, the performance of RBPT assays and the buffered acidified plate antigen test was estimated as relative sensitivity, accuracy, kappa agreement with CFT and association with CFT based on Pearson's chi-squared test and Phi coefficient. Almost all acidified agglutination test formats were generally associated with CFT. The best performance of RBPT in cows, buffalo cows, ewes, female goats and she-camels was reached by the Scottish, the French, the French, the UK and the Scottish RBPT respectively. From the animal inter-species perspective, the performance of the BCT 8% considerably fell in buffaloes and goats improving greatly with its 3% version, but causing extra drop in goats. The performance of the UK RBPT dropped in sheep and drastically fell in camels with its modification achieving some progress in buffaloes, sheep and camels, and minor drop in cattle and goats. The Scottish RBPT slightly dropped in buffaloes but noticeably in goats with good performance in cattle and camels. The French RBPT drastically fell in goats and less severely in camels. It was concluded that enhancing the sensitivity of the RBPT resulted in overall performance promotion especially in sheep, but still insufficiently accurate requiring some intervention in goats, buffaloes and camels in descending order of urgency.

Key words: BCT, RBPT, CFT, Brucella, ruminants, performance


 
ARTICLE TOOLS
Abstract
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ashraf Ezz El-Deen Sayour
Articles by Essam Mohamed Elbauomy
Articles by Nour Hosny Abdel-Hamid
Articles by Mohammad Kamal El-Kholi
Articles by Rania Ibrahim Ismail
Articles by Eman Ibrahim Mahmoud Beleta
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. 0; 0(0): -.

Web Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. https://www.alexjvs.com/?mno=262376 [Access: December 16, 2023].

AMA (American Medical Association) Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. 0; 0(0): -.


Vancouver/ICMJE Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. (0), [cited December 16, 2023]; 0(0): -.


Harvard Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta (0) Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS, 0 (0), -.


Turabian Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. 0. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. Alexandria Journal of Veterinary Sciences, 0 (0), -.


Chicago Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. "Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants." Alexandria Journal of Veterinary Sciences 0 (0), -.


MLA (The Modern Language Association) Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta. "Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print.


APA (American Psychological Association) Style

Ashraf Ezz El-Deen Sayour, Essam Mohamed Elbauomy, Nour Hosny Abdel-Hamid, Mohammad Kamal El-Kholi, Rania Ibrahim Ismail, Eman Ibrahim Mahmoud Beleta (0) Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. Alexandria Journal of Veterinary Sciences, 0 (0), -.




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine, Alexandria University
    Edfina, Behaira, Egypt.
    PO.Box: 22758
    Telefax: +20 45 2963526
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use