E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 12 May 2017
 


Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants

Ashraf E. Sayour, Essam M. Elbauomy, Nour H. Abdel-hamid, Mohammad K. El-kholi, Rania I. Ismail And Eman I. M. Beleta.


Abstract
A total of 543 sera from unvaccinated ruminant species, viz. cows, buffalo cows, ewes, female goats and she-camels in seven Nile Delta governorates were tested. Most animals had a history of Brucella melitensis biovar 3 infection. This study was to investigate the fitness of Rose-Bengal plate test (RBPT) formats namely the US brucellosis card (BCT) 8% and 3%, the UK RBPT and its modification, the Scottish RBPT and the French RBPT 4.5% for pre-confirmation of B. melitensis infection in non-bovine ruminants. Taking the complement fixation test (CFT) as the gold standard, the performance of RBPT assays and the buffered acidified plate antigen test was estimated as relative sensitivity, accuracy, kappa agreement with CFT and association with CFT based on Pearson's chi-squared test and Phi coefficient. Almost all acidified agglutination test formats were generally associated with CFT. The best performance of RBPT in cows, buffalo cows, ewes, female goats and she-camels was reached by the Scottish, the French, the French, the UK and the Scottish RBPT respectively. From the animal inter-species perspective, the performance of the BCT 8% considerably fell in buffaloes and goats improving greatly with its 3% version, but causing having extra drop in goats. The performance of the UK RBPT dropped in sheep and drastically fell in camels with its modification achieving some progress in buffaloes, sheep and camels, and minor drop in cattle and goats. The Scottish RBPT slightly dropped in buffaloes but noticeably in goats with good performance in cattle and camels. The French RBPT drastically fell in goats and less severely in camels. It was concluded that enhancing the sensitivity of the RBPT resulted in overall performance promotion especially in sheep, but still insufficiently accurate requiring some intervention in goats, buffaloes and camels in descending order of urgency

Key words: BCT, RBPT, CFT, Brucella, cow, buffalo, ewe, goat, camel.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ashraf E. Sayour
Articles by Essam M. Elbauomy
Articles by Nour H. Abdel-Hamid
Articles by Mohammad K. El-Kholi
Articles by Rania I. Ismail and Eman I. M. Beleta
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Sayour AE, Elbauomy EM, Abdel-hamid NH, El-kholi MK, . Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. 2017; 53(1): 187-202. doi:10.5455/ajvs.266638

Web Style

Sayour AE, Elbauomy EM, Abdel-hamid NH, El-kholi MK, . Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. https://www.alexjvs.com/?mno=266641 [Access: March 25, 2025]. doi:10.5455/ajvs.266638

AMA (American Medical Association) Style

Sayour AE, Elbauomy EM, Abdel-hamid NH, El-kholi MK, . Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. 2017; 53(1): 187-202. doi:10.5455/ajvs.266638


Vancouver/ICMJE Style

Sayour AE, Elbauomy EM, Abdel-hamid NH, El-kholi MK, . Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS. (2017), [cited March 25, 2025]; 53(1): 187-202. doi:10.5455/ajvs.266638


Harvard Style

Sayour, A. E., Elbauomy, . E. M., Abdel-hamid, . N. H., El-kholi, . M. K. & (2017) Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. AJVS, 53 (1), 187-202. doi:10.5455/ajvs.266638


Turabian Style

Sayour, Ashraf E., Essam M. Elbauomy, Nour H. Abdel-hamid, Mohammad K. El-kholi, and Rania I. Ismail And Eman I. M. Beleta. 2017. Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. Alexandria Journal of Veterinary Sciences, 53 (1), 187-202. doi:10.5455/ajvs.266638


Chicago Style

Sayour, Ashraf E., Essam M. Elbauomy, Nour H. Abdel-hamid, Mohammad K. El-kholi, and Rania I. Ismail And Eman I. M. Beleta. "Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants." Alexandria Journal of Veterinary Sciences 53 (2017), 187-202. doi:10.5455/ajvs.266638


MLA (The Modern Language Association) Style

Sayour, Ashraf E., Essam M. Elbauomy, Nour H. Abdel-hamid, Mohammad K. El-kholi, and Rania I. Ismail And Eman I. M. Beleta. "Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants." Alexandria Journal of Veterinary Sciences 53.1 (2017), 187-202. Print. doi:10.5455/ajvs.266638


APA (American Psychological Association) Style

Sayour, A. E., Elbauomy, . E. M., Abdel-hamid, . N. H., El-kholi, . M. K. & (2017) Validation of Different Versions of The Card or Rose-Bengal Test for The Diagnosis of Brucella melitensis Infection in Ruminants. Alexandria Journal of Veterinary Sciences, 53 (1), 187-202. doi:10.5455/ajvs.266638




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use