Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli

Samar A. Swelam; Abbas A. Amin; Amer A. Amer; Hussin S. Abo El-Makarem

doi:10.5455/ajvs.267275

2025, Vol: 86

86Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Original Article

AJVS. 2017; 54(2): 99-105

doi: 10.5455/ajvs.267275

Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli

Samar A. Swelam, Abbas A. Amin, Amer A. Amer, Hussin S. Abo El-makarem.

Abstract
Milk as it comes from a normal udder of a healthy animal contains a negligible number of harmful organisms but may be contaminated from variable sources. Forty random samples of raw milk were collected from supermarkets in Alexandria governorate for isolation and identification of some pathogenic microorganisms using conventional methods and advanced techniques. incidence of E. coli, Staph.aureus, listeria monocytogens and salmonella were 80, 45, 5 and 0%, respectively by using plating techniques. These results were confirmed by conventional biochemical identifications but by using vitek 2 compact system (automated biochemical identifications), the incidenceswere 37.5, 2.5, 0 and 0%, respectively.The incidence of Shiga toxin-producing E. coli isolated from the examined raw milk samples (4/10%) and could be identify virulence genes intofour different serotypes including O26:H11 (EHEC), O86 (EHEC), O119:H6 (ETEC) and O125:H21 (EHEC). By using multiplex PCR, it was found that O26:H11 had shigatoxin 1 gene, shigatoxin 2 gene and intimin gene; while O86 and O119:H6 serotypes had only shigatoxin1 gene. O125:H21 serotype had both shigatoxin 1 and shigatoxin 2 genes. It could be concluded that vitek 2 compact system is considered as sensitive and accurate method for identification of some food poisoning microorganisms in comparing with conventional biochemical identifications.

Key words: E. coli, Staph.aureus, listeria monocytogenes, raw milk, viteck 2 compact

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Samar A. Swelam Articles by Abbas A. Amin Articles by Amer A. Amer Articles by Hussin S. Abo El-Makarem
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275

Web Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. https://www.alexjvs.com/?mno=267275 [Access: May 04, 2025]. doi:10.5455/ajvs.267275

AMA (American Medical Association) Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275

Vancouver/ICMJE Style

Swelam SA, Amin AA, Amer AA, El-makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. (2017), [cited May 04, 2025]; 54(2): 99-105. doi:10.5455/ajvs.267275

Harvard Style

Swelam, S. A., Amin, . A. A., Amer, . A. A. & El-makarem, . H. S. A. (2017) Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS, 54 (2), 99-105. doi:10.5455/ajvs.267275

Turabian Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-makarem. 2017. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. Alexandria Journal of Veterinary Sciences, 54 (2), 99-105. doi:10.5455/ajvs.267275

Chicago Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-makarem. "Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli." Alexandria Journal of Veterinary Sciences 54 (2017), 99-105. doi:10.5455/ajvs.267275

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article