E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
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Original Article 


Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk

Fawzi O. Ebrahim, Abdul-munam M. Fellah, Saber Eldarhobi, Adam Elzagheid.

Abstract
Human and bovine lactoferrin have been studied extensively, but very few reports exist on camel (Camelus dromedarius) lactoferrin. This iron-binding glycoprotein is present primarily in milk. It has been shown to be involved in various physiological and protective functions, including homeostasis and cell proliferation, and it has antibacterial, antifungal, antiviral, antioxidant, immunomodulatory and anticancer activities. This study aimed to compare the protein profiles of camel milk and bovine milk by gel electrophoresis and to isolate camel lactoferrin from colostrum. Camel milk proteins profile lacked β-lactoglobulin. Lactoferrin was isolated from colostrum by cation exchange chromatography and identified by its molecular weight after gel electrophoresis as a single band of about 78 kDa, demonstrating the purity of the isolated protein. The study demonstrates a simple one-step method to purify lactoferrin from camel colostrum.

Key words: Lactoferrin (Lf), Camel Lactoferrin (CLf), Colostrum, Cation Exchange Chromatography (CEC)


 
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How to Cite this Article
Pubmed Style

Ebrahim FO, Fellah AM, Eldarhobi S, Elzagheid A. Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. AJVS. 2019; 60(2): 67-70. doi:10.5455/ajvs.27168

Web Style

Ebrahim FO, Fellah AM, Eldarhobi S, Elzagheid A. Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. https://www.alexjvs.com/?mno=27168 [Access: December 03, 2023]. doi:10.5455/ajvs.27168

AMA (American Medical Association) Style

Ebrahim FO, Fellah AM, Eldarhobi S, Elzagheid A. Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. AJVS. 2019; 60(2): 67-70. doi:10.5455/ajvs.27168


Vancouver/ICMJE Style

Ebrahim FO, Fellah AM, Eldarhobi S, Elzagheid A. Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. AJVS. (2019), [cited December 03, 2023]; 60(2): 67-70. doi:10.5455/ajvs.27168


Harvard Style

Ebrahim, F. O., Fellah, . A. M., Eldarhobi, . S. & Elzagheid, . A. (2019) Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. AJVS, 60 (2), 67-70. doi:10.5455/ajvs.27168


Turabian Style

Ebrahim, Fawzi O., Abdul-munam M. Fellah, Saber Eldarhobi, and Adam Elzagheid. 2019. Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. Alexandria Journal of Veterinary Sciences, 60 (2), 67-70. doi:10.5455/ajvs.27168


Chicago Style

Ebrahim, Fawzi O., Abdul-munam M. Fellah, Saber Eldarhobi, and Adam Elzagheid. "Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk." Alexandria Journal of Veterinary Sciences 60 (2019), 67-70. doi:10.5455/ajvs.27168


MLA (The Modern Language Association) Style

Ebrahim, Fawzi O., Abdul-munam M. Fellah, Saber Eldarhobi, and Adam Elzagheid. "Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk." Alexandria Journal of Veterinary Sciences 60.2 (2019), 67-70. Print. doi:10.5455/ajvs.27168


APA (American Psychological Association) Style

Ebrahim, F. O., Fellah, . A. M., Eldarhobi, . S. & Elzagheid, . A. (2019) Purification of Lactoferrin from Camel colostrum and Protein Profiles of Camel and Bovine Milk. Alexandria Journal of Veterinary Sciences, 60 (2), 67-70. doi:10.5455/ajvs.27168




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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