E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates

Marwa F. Abd El-Kader 1 and Tarek M. Mousa-Balabel.

Cited by (1)

Abstract
In the last years, there were some bacterial outbreaks occurred during the summer season of farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates resulting in high fish mortalities and severe economic losses in these farms. So, this study aimed to detect the causes of the summer fish mortalities in these governorates to facilitate the diagnosis methods of study pathogens. Thirty fish farms were surveyed (20 in Kafr El-Sheikh and 10 in Dakahlia). 150 examined diseased fish (five from each farm) were succumbed for bacteriological isolation and biochemical and molecular identification. LD50 experiments were done to detect the infectivity and pathogenicity of some strains of bacterial isolates. The obtained results cleared that O. niloticus summer mortalities were caused by mixed infections with different bacterial species. The isolated bacterial species were 9 types of bacteria; 7 gram negative bacteria; 78 (30.5%) Aeromonas hydrophila, 52 (20.3%) Pseudomonas aeruginosa, 40 (15.6%) Vibrio anguillarum, 20 (7.8%) Edwardsiella tarda, 12 (4.7%) Edwardsiella ictulari, 10 (3.9%) Pseudomonas fluorescens, 7 (2.7%) Flavobacterium columnare and 2 gram positive bacteria; 30 (11.7%) Streptococcus agalactia and 7 (2.8) Enterococcus feacalis which were the most prevalent bacteria affecting fish farms and implicated in the O. niloticus seasonal summer mortalities and LD50 experiments confirmed the pathogenicity of some isolated bacteria. From the obtained results, it could be concluded that, the main cause of summer fish mortalities in studied farms was 81.82% gram negative bacteria and 18.18% was gram positive bacteria.

Key words: Infectivity, LD50, Oreochromis niloticus, summer mortalities.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Marwa F. Abd El-Kader 1 and Tarek M. Mousa-Balabel
on Google
on Google Scholar


This Article Cited By the following articles

Virulence genes contributing to Aeromonas hydrophila pathogenicity in Oreochromis niloticus
Int Microbiol 2019; (): .

1
 
How to Cite this Article
Pubmed Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . AJVS. 2017; 53(2): 107-113. doi:10.5455/ajvs.265631


Web Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . http://www.alexjvs.com/?mno=272886 [Access: August 18, 2021]. doi:10.5455/ajvs.265631


AMA (American Medical Association) Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . AJVS. 2017; 53(2): 107-113. doi:10.5455/ajvs.265631



Vancouver/ICMJE Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . AJVS. (2017), [cited August 18, 2021]; 53(2): 107-113. doi:10.5455/ajvs.265631



Harvard Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel (2017) Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . AJVS, 53 (2), 107-113. doi:10.5455/ajvs.265631



Turabian Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. 2017. Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . Alexandria Journal of Veterinary Sciences, 53 (2), 107-113. doi:10.5455/ajvs.265631



Chicago Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. "Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates ." Alexandria Journal of Veterinary Sciences 53 (2017), 107-113. doi:10.5455/ajvs.265631



MLA (The Modern Language Association) Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel. "Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates ." Alexandria Journal of Veterinary Sciences 53.2 (2017), 107-113. Print. doi:10.5455/ajvs.265631



APA (American Psychological Association) Style

Marwa F. Abd El-Kader and Tarek M. Mousa-Balabel (2017) Isolation and Molecular Characterization of Some Bacteria Implicated in the Seasonal Summer Mortalities of Farm-raised Oreochromis niloticus at Kafr El-Sheikh and Dakahlia Governorates . Alexandria Journal of Veterinary Sciences, 53 (2), 107-113. doi:10.5455/ajvs.265631





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]