E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 86
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Original Article
Online Published: 14 Sep 2025
 


Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing

Ahmed. M. Shalaby, Samy, A. Khalil, Mohamed M. Elsenduony.


Abstract
This study aimed to investigate the prevalence, antibiotic resistance, and genetic relationships of Staphylococcus aureus isolated from frozen and minced meat products in Alexandria, Egypt. A total of 200 samples (100 frozen meat and 100 minced frozen meat) were collected from local supermarkets at Alexandria City, Egypt. Conventional methods beside VITEK 2 system were used for identification, while antibiotic susceptibility testing was performed to detect Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Staphylococcus aureus (VRSA). Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was employed to analyze Genetic diversity. Lastly, gene sequencing was conducted for antibiotic resistance genes (mecA, vanA, and vanB). Results showed that 7% of frozen meat and 8% of minced meat samples were positive for coagulase positive Staphylococcus aureus using conventional methods and VITEK confirmation system. Among Staphylococcus aureus isolates, 57.1% from frozen meat and 50% from minced meat were MRSA, with one VRSA isolate from each category at incidence rate 14.3 and 12.5%, respectively. ERIC-PCR revealed high genetic similarity (>95%) among some isolates, suggesting a common source of contamination. Sequencing confirmed high similarity (97.42%) of the mecA gene with known MRSA strains. These findings highlight the need for improved food safety measures to prevent the spread of antibiotic-resistant S. aureus in the food supply chain.

Key words: Staphylococcus.aureus, Frozen, minced meat, MRSA, VRSA, antibiotic resistance genes, ERIC-PCR


 
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Pubmed Style

Shalaby AM, Samy , Khalil A, Elsenduony MM. Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. AJVS. Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143

Web Style

Shalaby AM, Samy , Khalil A, Elsenduony MM. Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. https://www.alexjvs.com/?mno=275143 [Access: September 20, 2025]. doi:10.5455/ajvs.275143

AMA (American Medical Association) Style

Shalaby AM, Samy , Khalil A, Elsenduony MM. Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. AJVS. Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143


Vancouver/ICMJE Style

Shalaby AM, Samy , Khalil A, Elsenduony MM. Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. AJVS, [cited September 20, 2025]; Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143


Harvard Style

Shalaby, A. M., Samy, ., Khalil, . A. & Elsenduony, . M. M. (0) Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. AJVS, Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143


Turabian Style

Shalaby, Ahmed. M., Samy, A. Khalil, and Mohamed M. Elsenduony. 0. Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. Alexandria Journal of Veterinary Sciences, Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143


Chicago Style

Shalaby, Ahmed. M., Samy, A. Khalil, and Mohamed M. Elsenduony. "Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing." Alexandria Journal of Veterinary Sciences Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143


MLA (The Modern Language Association) Style

Shalaby, Ahmed. M., Samy, A. Khalil, and Mohamed M. Elsenduony. "Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing." Alexandria Journal of Veterinary Sciences Online First: 14 Sep, 2025. Web. 20 Sep 2025 doi:10.5455/ajvs.275143


APA (American Psychological Association) Style

Shalaby, A. M., Samy, ., Khalil, . A. & Elsenduony, . M. M. (0) Molecular Characterization and Genetic Diversity of Methicillin- and Vancomycin-Resistant Staphylococcus aureus from Frozen and Minced Meat Using ERIC-PCR and Gene Sequencing. Alexandria Journal of Veterinary Sciences, Online First: 14 Sep, 2025. doi:10.5455/ajvs.275143




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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