Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese

Ashraf M. Nazem; Mohamed S. Dahmash; Riad R. Kahlil

doi:10.5455/ajvs.27558

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Original Article

AJVS. 2019; 60(1): 161-166

doi: 10.5455/ajvs.27558

Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese

Ashraf M. Nazem, Mohamed S. Dahmash, Riad R. Kahlil.

Abstract
Cheese is a histamine-rich food and may contain biogenic amines formed during ripening process, due to degradation of proteins through proteolysis. Therefore, histamine producing bacteria and biogenic amines content were studied during ripening of ras cheese at refrigeration temperature 4±1oC for 10 weeks using HPLC. Three batches of Ras cheese were manufactured using Egyptian conventional method. From each batch, samples collected at zero, 2, 4, 6, 8 and 10 weeks after cheese manufacture. The obtained results revealed that Enterobacteriaceae and Enterococci had the same pattern during ripening of ras cheese with a significant decrease from (5.0x108±7.02x106 at zero day until reach 4.60x10±0.24x10 cfu/g at week 10 of storage), (from 3.46x105±3.17x104 at day zero until reach to 2.20x10±0.23x10 cfu/g at week 10 of storage), respectively. Lactobacilli count showed fluctuation during ripening period where the highest count recorded at 2nd and 10th week 4.40x105±0.25x105 and 4.40x105±0.15x105 cfu/g, respectively. Enterobacteriaceae, enterococci and lactobacilli producing histamine were identified. Investigated biogenic amines (Cadaverine, Histamine and Tyramine) significantly decrease during ripening of ras cheese while, Putrescine showing fluctuation during ripening. Therefore, to protect consumers from biogenic amines poisoning, factors that enhance biogenic amines formation during cheese processing should be controlled.

Key words: Ras cheese, histamine- producing bacteria, biogenic amines, HPLC

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Pubmed Style

Nazem AM, Dahmash MS, Kahlil RR. Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. AJVS. 2019; 60(1): 161-166. doi:10.5455/ajvs.27558

Web Style

Nazem AM, Dahmash MS, Kahlil RR. Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. https://www.alexjvs.com/?mno=27558 [Access: May 04, 2025]. doi:10.5455/ajvs.27558

AMA (American Medical Association) Style

Nazem AM, Dahmash MS, Kahlil RR. Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. AJVS. 2019; 60(1): 161-166. doi:10.5455/ajvs.27558

Vancouver/ICMJE Style

Nazem AM, Dahmash MS, Kahlil RR. Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. AJVS. (2019), [cited May 04, 2025]; 60(1): 161-166. doi:10.5455/ajvs.27558

Harvard Style

Nazem, A. M., Dahmash, . M. S. & Kahlil, . R. R. (2019) Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. AJVS, 60 (1), 161-166. doi:10.5455/ajvs.27558

Turabian Style

Nazem, Ashraf M., Mohamed S. Dahmash, and Riad R. Kahlil. 2019. Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. Alexandria Journal of Veterinary Sciences, 60 (1), 161-166. doi:10.5455/ajvs.27558

Chicago Style

Nazem, Ashraf M., Mohamed S. Dahmash, and Riad R. Kahlil. "Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese." Alexandria Journal of Veterinary Sciences 60 (2019), 161-166. doi:10.5455/ajvs.27558

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Nazem, A. M., Dahmash, . M. S. & Kahlil, . R. R. (2019) Influence of Refrigeration on Histamine-Producing Bacteria and Biogenic Amines Content During Ripening of Ras Cheese. Alexandria Journal of Veterinary Sciences, 60 (1), 161-166. doi:10.5455/ajvs.27558

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