E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 82
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Original Article 


Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman.


Abstract
Brucellosis is a worldwide zoonotic disease that causes major public health hazard in Egypt. The present work was conducted to investigate the prevalence of bovine brucellosis on the basis of serological test RBPT and MRT in serum and milk samples of lactating dairy animals, also isolation of brucella organism from milk samples, PCR amplification was done for detection of Brucella abortus DNA using BCSP31 target gene. 219 serum samples from sheep and goat and 119 milk samples from cow, sheep, and goat were collected from a governmental and private farm. The prevalence of brucellosis by RBPT was 34.5 and 61.4% in serum samples of sheep and goat also, by MRT were 2.3, 13.8 and 10% in milk samples of cow, sheep, and goat, respectively. Milk samples were cultured on the Brucella agar medium and gave the same prevalence rate. A specific PCR assay targeting the bcsp-31 gene within the genome of Brucella species has been used and gave an amplicon of 223 bp in three strain of isolated brucella. It is concluded that RBPT and MRT used as screening test for detection prevalence of brucella species in serum and milk serum, PCR assay consider a useful tool for detection Brucella species in milk.

Key words: Brucella species, RBPT, MRT, bcsp-31 gene


 
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How to Cite this Article
Pubmed Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. 2017; 55(2): 19-24. doi:10.5455/ajvs.278394

Web Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. https://www.alexjvs.com/?mno=278394 [Access: September 12, 2024]. doi:10.5455/ajvs.278394

AMA (American Medical Association) Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. 2017; 55(2): 19-24. doi:10.5455/ajvs.278394


Vancouver/ICMJE Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS. (2017), [cited September 12, 2024]; 55(2): 19-24. doi:10.5455/ajvs.278394


Harvard Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman (2017) Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. AJVS, 55 (2), 19-24. doi:10.5455/ajvs.278394


Turabian Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. 2017. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. Alexandria Journal of Veterinary Sciences, 55 (2), 19-24. doi:10.5455/ajvs.278394


Chicago Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. "Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals." Alexandria Journal of Veterinary Sciences 55 (2017), 19-24. doi:10.5455/ajvs.278394


MLA (The Modern Language Association) Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman. "Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals." Alexandria Journal of Veterinary Sciences 55.2 (2017), 19-24. Print. doi:10.5455/ajvs.278394


APA (American Psychological Association) Style

Ayman S. Nofal, Ahlam A. El-Leboudy, Hussein S. Abo El-Makarem, Amir H.Abd El-Rahman (2017) Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. Alexandria Journal of Veterinary Sciences, 55 (2), 19-24. doi:10.5455/ajvs.278394




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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