E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms

Aml M. Ragab, Amany Dapgh, Huda ELsayed.

Abstract
Bacterial resistance has a major role in the failure of infectious illness treatments. E.coli is one of the most common causes of mastitis, so a total of 100 samples (50 each from milk and feces) were collected from dairy cows from various farms in Gharbia Governorate Egypt, to determine the prevalence of E.coli, isolates were analyzed for antibiotic susceptibility. The purpose of our research was to assess the antibacterial activity and mechanism of action of two nanoemulsion EOs (Citronella and Mentha spicata) against of Escherichia coli. Antibacterial action was assessed by utilizing the agar well diffusion technique, with an electron microscope used to confirm the effect of minimum inhibitory concentration (MIC) on tested bacterial cell and minimum bactericidal concentration (MBC). E. coli was more susceptible to nanoemulsion EOs with a larger inhibition zone diameter; the present research demonstrates the antimicrobial activity that nanoemulsion EOs have against the tested bacteria by acting on the cell surface and disrupting the cell membrane; bacterial cells treated with both essential oils nanoemulsions demonstrated varying degrees of deformation and disruption. On the surfaces of strains treated with 2 MIC, which was substantially more effective than MIC, considerable morphological alterations were observed.

Key words: Key words: E.Coli, Citronella, Mentha spicata, Essential oils and Electron microscope.


 
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How to Cite this Article
Pubmed Style

Ragab AM, Dapgh A, ELsayed H. The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. AJVS. 2022; 72(1): 22-34. doi:10.5455/ajvs.29576


Web Style

Ragab AM, Dapgh A, ELsayed H. The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. https://www.alexjvs.com/?mno=29576 [Access: September 01, 2022]. doi:10.5455/ajvs.29576


AMA (American Medical Association) Style

Ragab AM, Dapgh A, ELsayed H. The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. AJVS. 2022; 72(1): 22-34. doi:10.5455/ajvs.29576



Vancouver/ICMJE Style

Ragab AM, Dapgh A, ELsayed H. The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. AJVS. (2022), [cited September 01, 2022]; 72(1): 22-34. doi:10.5455/ajvs.29576



Harvard Style

Ragab, A. M., Dapgh, . A. & ELsayed, . H. (2022) The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. AJVS, 72 (1), 22-34. doi:10.5455/ajvs.29576



Turabian Style

Ragab, Aml M., Amany Dapgh, and Huda ELsayed. 2022. The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. Alexandria Journal of Veterinary Sciences, 72 (1), 22-34. doi:10.5455/ajvs.29576



Chicago Style

Ragab, Aml M., Amany Dapgh, and Huda ELsayed. "The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms." Alexandria Journal of Veterinary Sciences 72 (2022), 22-34. doi:10.5455/ajvs.29576



MLA (The Modern Language Association) Style

Ragab, Aml M., Amany Dapgh, and Huda ELsayed. "The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms." Alexandria Journal of Veterinary Sciences 72.1 (2022), 22-34. Print. doi:10.5455/ajvs.29576



APA (American Psychological Association) Style

Ragab, A. M., Dapgh, . A. & ELsayed, . H. (2022) The Antibacterial activity of some Essential Oils Nano Particles on Escherichia coli infection in dairy farms. Alexandria Journal of Veterinary Sciences, 72 (1), 22-34. doi:10.5455/ajvs.29576





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