E-ISSN 2536-9520 | ISSN 1110-2047
 
2026, Vol: 89
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Original Article
Online Published: 14 Mar 2026
 


Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit

Fatma M. Gadallah, Fatma Elzahraa G. Abo-elkhir, Ahmed M. Abdelhamid, Selim S. Salama.


Abstract
Bacterial diseases remain a major constraint to intensive rabbit production, with pasteurellosis representing a leading cause of mortality and substantial economic losses. In addition to Pasteurella multocida, members of the family Enterobacteriaceae, particularly Escherichia coli, are frequently associated with fatal infections in rabbits. In the present study, a locally formulated, combination inactivated vaccine containing E. coli and P. multocida adjuvanted with Montanide ISA 70, was developed and its immunogenicity and protective efficacy were evaluated in rabbits. A total of 370 rabbits were divided into 4 experimental groups: polyvalent inactivated P. multocida vaccine, polyvalent inactivated E. coli vaccine, combination inactivated P. multocida and E. coli vaccine, and unvaccinated controls. All vaccinated rabbits received two subcutaneous doses (0.5 ml) administered one month apart. Three weeks after the booster dose, animals were challenged with virulent strains of P. multocida or E. coli. Vaccine-induced humoral immune responses were assessed using indirect hemagglutination and enzyme-linked immunosorbent assay. The protective effectiveness was assessed by challenge trials and determination of LD₅₀. Combined inactivated vaccine elicited the highest antibody responses and conferred up to 90% protection against challenge with virulent P. multocida serotypes A and D and E. coli serotypes O157, O151, and O125. In comparison, monovalent vaccines induced lower levels of protection. These findings demonstrate that the combination inactivated P. multocida and E. coli vaccine is immunogenic and effective, supporting its potential application for controlling pasteurellosis and colibacillosis in rabbit production systems.

Key words: Rabbit protection, P. multocida, E. coli, Challenge test, Vaccine, ELISA.


 
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How to Cite this Article
Pubmed Style

Gadallah FM, Abo-elkhir FEG, Abdelhamid AM, Salama SS. Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. AJVS. 2026; 89(0): 27-35. doi:10.5455/ajvs.310946

Web Style

Gadallah FM, Abo-elkhir FEG, Abdelhamid AM, Salama SS. Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. https://www.alexjvs.com/?mno=310946 [Access: April 16, 2026]. doi:10.5455/ajvs.310946

AMA (American Medical Association) Style

Gadallah FM, Abo-elkhir FEG, Abdelhamid AM, Salama SS. Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. AJVS. 2026; 89(0): 27-35. doi:10.5455/ajvs.310946


Vancouver/ICMJE Style

Gadallah FM, Abo-elkhir FEG, Abdelhamid AM, Salama SS. Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. AJVS. (2026), [cited April 16, 2026]; 89(0): 27-35. doi:10.5455/ajvs.310946


Harvard Style

Gadallah, F. M., Abo-elkhir, . F. E. G., Abdelhamid, . A. M. & Salama, . S. S. (2026) Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. AJVS, 89 (0), 27-35. doi:10.5455/ajvs.310946


Turabian Style

Gadallah, Fatma M., Fatma Elzahraa G. Abo-elkhir, Ahmed M. Abdelhamid, and Selim S. Salama. 2026. Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. Alexandria Journal of Veterinary Sciences, 89 (0), 27-35. doi:10.5455/ajvs.310946


Chicago Style

Gadallah, Fatma M., Fatma Elzahraa G. Abo-elkhir, Ahmed M. Abdelhamid, and Selim S. Salama. "Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit." Alexandria Journal of Veterinary Sciences 89 (2026), 27-35. doi:10.5455/ajvs.310946


MLA (The Modern Language Association) Style

Gadallah, Fatma M., Fatma Elzahraa G. Abo-elkhir, Ahmed M. Abdelhamid, and Selim S. Salama. "Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit." Alexandria Journal of Veterinary Sciences 89.0 (2026), 27-35. Print. doi:10.5455/ajvs.310946


APA (American Psychological Association) Style

Gadallah, F. M., Abo-elkhir, . F. E. G., Abdelhamid, . A. M. & Salama, . S. S. (2026) Development and Potency Assessment of a Locally Prepared Combined Inactivated Vaccine Against Pasteurella Multocida and Escherichia Coli in Rabbit. Alexandria Journal of Veterinary Sciences, 89 (0), 27-35. doi:10.5455/ajvs.310946




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110-204 ... Read more.



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    Alexandria University
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    Abees 10th district, Alexandria
    Egypt.

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