E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES

Ayman Mohammed Kamar, Mohammed El senduouny, Diaa Ezzat Hussein.

Abstract
This investigation was carried out to investigate the relationship between incidence of multidrug-resistant isolates and presence of class 1 integron in Salmonella recovered from poultry from local Egyptian markets. One hundred samples of raw chicken carcasses which obtained from ten poultry markets at Alexandria markets were examined for presence of Salmonella sp., via conventional microbiological methods, serotyping, real time PCR and lastly Conventional PCR for screening of Salmonella for sequencing and Class-1 Integrons presence. Detection rate of Salmonella sp. was (6%) with serotypes S. kentucky, S. typhimurium, S. minnesota and S. arizonae with positive results for Salmonella for conventional and quantitative PCR. Based on sequenced specific amplicons with 204 bp, Salmonella isolate no.1 was more identical to isolate no.2 which showed highest similarity to isolate no.4, while Isolate no.3 was more identical to isolate no.6, Isolate no.4 was more identical to isolate no.2, Isolate no.5 was more identical to isolate no.4. Lastly, isolate no.6 was more identical to isolate no.3. comparing with gene bank database, first, second, third, fourth, fifth and sixth isolates identified as S. kentucky, S. minnesota, S. kentucky, S. minnesota and S. typhimurium and S. arizonae respectively. Our obtaining results indicated that all six isolates considered multi-resistance isolates as (100 %) with varied resistance patterns. Class-1 Integron detection results reflected that first, second and fourth samples were Positive for class 1 integron (750 bp) which indicated relationship between antibiotic resistance and presence of integron in bacteria. Our obtaining findings cleared correlation between high antibiotics resistance rates exhibited by Salmonella isolates and presence of class 1 integron gene cassettes harboring resistance genes.

Key words: Salmonella Phenotypic - Genotypic Serotyping quantitative PCR multi-resistance isolates - Class-1 Integrons.


 
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Pubmed Style

Kamar AM, senduouny ME, Hussein DE, . MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.


Web Style

Kamar AM, senduouny ME, Hussein DE, . MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES. http://www.alexjvs.com/?mno=58254 [Access: March 31, 2021].


AMA (American Medical Association) Style

Kamar AM, senduouny ME, Hussein DE, . MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.



Vancouver/ICMJE Style

Kamar AM, senduouny ME, Hussein DE, . MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.



Harvard Style

Kamar, A. M., senduouny, M. E., Hussein, D. E. & (0) MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.



Turabian Style

Kamar, Ayman Mohammed, Mohammed El senduouny, Diaa Ezzat Hussein, and . 0. MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.



Chicago Style

Kamar, Ayman Mohammed, Mohammed El senduouny, Diaa Ezzat Hussein, and . "MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES."



MLA (The Modern Language Association) Style

Kamar, Ayman Mohammed, Mohammed El senduouny, Diaa Ezzat Hussein, and . "MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES."



APA (American Psychological Association) Style

Kamar, A. M., senduouny, M. E., Hussein, D. E. & (0) MULTI ANTIBIOGRAM-INTEGRON RELATIONSHIP IN SALMONELLASPECIES ISOLATED FROM LOCAL POULTRY CARCASSES.





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