E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay

Diaa Eldin E. Hussein and Amr Ghit.

Abstract
Escherichia coli, the most important foodborne pathogen worldwide, can enter a dormant state called viable but nonculturable (VBNC) state under various stress conditions. Hence, it escapes detection by traditional culturing methods. The present study used Propidium monoazide (PMA)-Polymerase chain reaction (PCR) assay in order to verify whether E. coli, especially O157 strains, converted to the VBNC state under the influence of cool-warm and freeze-thaw stresses. 120 beef liver samples were collected from local markets. The samples that were positive to pathogenic E. coli were treated with cooling (4 C)-warm and freezing (-18 C)-thaw cycles to induce the VBNC state. Samples that demonstrated the inability to form colonies on EMB agar were tested by PMA-PCR assays to confirm the existence of VBNC E. coli. 9 samples (7.5 %) of 120 beef liver samples were positive to pathogenic E. coli by microbiological tests. Cool-warm and freeze-thaw stresses on the 9 samples led to the inability of 3 of them to grow on EMB agar plates. By using PMA-PCR assay, we found that 4 C cool-warm cycle induced E. coli into VBNC state at a higher percentage ratio than -18 C freeze-thaw cycle. Even though further experiments are still required, in rare cases, at -18 C, E. coli VBNC may lose expression of genes responsible for viability and metabolic activity. Additionally, our study evaluated the PMA-PCR assay as a rapid and accurate method to verify the existence of VBNC-associated foodborne E. coli O157.

Key words: E. coli O157; Food-borne diseases; freeze-thaw cycle; Polymerase chain reaction; Propidium Monoazide; VBNC state


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Diaa Eldin E. Hussein and Amr Ghit
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Diaa Eldin E. Hussein and Amr Ghit. Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. AJVS. 2021; 69(2): 1-6. doi:10.5455/ajvs.75980


Web Style

Diaa Eldin E. Hussein and Amr Ghit. Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. http://www.alexjvs.com/?mno=75980 [Access: August 17, 2021]. doi:10.5455/ajvs.75980


AMA (American Medical Association) Style

Diaa Eldin E. Hussein and Amr Ghit. Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. AJVS. 2021; 69(2): 1-6. doi:10.5455/ajvs.75980



Vancouver/ICMJE Style

Diaa Eldin E. Hussein and Amr Ghit. Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. AJVS. (2021), [cited August 17, 2021]; 69(2): 1-6. doi:10.5455/ajvs.75980



Harvard Style

Diaa Eldin E. Hussein and Amr Ghit (2021) Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. AJVS, 69 (2), 1-6. doi:10.5455/ajvs.75980



Turabian Style

Diaa Eldin E. Hussein and Amr Ghit. 2021. Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. Alexandria Journal of Veterinary Sciences, 69 (2), 1-6. doi:10.5455/ajvs.75980



Chicago Style

Diaa Eldin E. Hussein and Amr Ghit. "Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay." Alexandria Journal of Veterinary Sciences 69 (2021), 1-6. doi:10.5455/ajvs.75980



MLA (The Modern Language Association) Style

Diaa Eldin E. Hussein and Amr Ghit. "Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay." Alexandria Journal of Veterinary Sciences 69.2 (2021), 1-6. Print. doi:10.5455/ajvs.75980



APA (American Psychological Association) Style

Diaa Eldin E. Hussein and Amr Ghit (2021) Detection of Viable but Non-Culturable (Vbnc) Escherichia Coli from Beef Liver Using Pma-PCR Assay. Alexandria Journal of Veterinary Sciences, 69 (2), 1-6. doi:10.5455/ajvs.75980





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 10 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 8 times [Click to see citing articles]

  • Effect of Dietary Replacement of Inorganic Zinc by Organic or Nanoparticles Sources on Growth Performance, Immune Response and Intestinal Histopathology of Broiler Chicken
    Mohamed El-Katcha, Mosad A. Soltan, M. El-badry
    AJVS. 2017; 55(2): 129-145
    » Abstract » doi: 10.5455/ajvs.266925
    Cited : 7 times [Click to see citing articles]

  • Molecular Characterization, Antimicrobial Susceptibility and Salt Tolerance of Aeromonas hydrophila from Fresh, Brackish and Marine fishes
    Basma Dahdouh, Ola Basha, Samy Khalil, Mahmoud Tanekhy
    AJVS. 2016; 48(2): 46-53
    » Abstract & References » doi: 10.5455/ajvs.208107
    Cited : 6 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 5 times [Click to see citing articles]